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[190509_at] serine/threonine kinase

Description C53A5.4 /REP_DB=WormBase Gene ID /WP=CE08953 /TR=O17693 /GB=CAB03982.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 87      4grp_total > 02      6grp_cgc > 58      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 294
1 2 3 4 range
A 45.4
(-3)
A 		63.2
(-2.6)
A 		53.8
(-2.8)
A 		308.4
(3)
P 		263
B 38.8
(-2.4)
A 		17.1
(-2.9)
A 		14.3
(-3)
A 		267.9
(3)
P 		254
C 38.4
(-2.9)
A 		36
(-3)
A 		39.1
(-2.9)
A 		258.1
(3)
P 		222
avr 40.9
(-2.9) 		38.8
(-2.9) 		35.7
(-3) 		278.1
(3) 		242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 11.5
(-3)
A 		49.7
(-1.1)
A 		23.8
(-2.4)
A 		28.2
(-2.2)
A 		60.7
(-0.6)
M 		133.9
(3)
P 		122
B 21.4
(-2.3)
A 		17.7
(-2.5)
A 		9.7
(-3)
A 		40.6
(-1.2)
A 		79.7
(1)
A 		114.4
(3)
P 		105
C 19.7
(-3)
A 		66.8
(-0.3)
A 		25
(-2.7)
A 		42.4
(-1.7)
A 		59.7
(-0.7)
A 		125.7
(3)
P 		106
avr 17.5
(-3) 		44.7
(-1.5) 		19.5
(-2.9) 		37.1
(-1.9) 		66.7
(-0.2) 		124.7
(3) 		107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 16.5
(-2.5)
A 		13.7
(-3)
A 		14.1
(-2.9)
A 		45.1
(3)
P 		31
B 12.4
(-2)
A 		4.2
(-2.9)
A 		3.3
(-3)
A 		57.1
(3)
P 		54
C 12.7
(-2.6)
A 		10.4
(-3)
A 		13.6
(-2.4)
A 		43.5
(3)
P 		33
avr 13.9
(-2.3) 		9.4
(-3) 		10.3
(-2.9) 		48.6
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 2.1
(-3)
A 		17.7
(0.1)
A 		5.2
(-2.4)
A 		7.2
(-2)
A 		18.3
(0.2)
M 		32.6
(3)
P 		31
B 7.4
(-2.2)
A 		5.9
(-2.5)
A 		2.3
(-3)
A 		10.8
(-1.7)
A 		32.4
(1.5)
A 		42.6
(3)
P 		40
C 7.3
(-2.8)
A 		20.4
(-0.6)
A 		6.4
(-3)
A 		8.5
(-2.6)
A 		19.3
(-0.8)
A 		42.1
(3)
P 		36
avr 5.6
(-2.8) 		14.7
(-1.2) 		4.6
(-3) 		8.8
(-2.3) 		23.3
(0.3) 		39.1
(3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190509_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13165
Sequence Type Exemplar sequence
Representative Public ID C53A5.4 NCBI
Target Description C53A5.4 /REP_DB=WormBase Gene ID /WP=CE08953 /TR=O17693 /GB=CAB03982.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074199(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074199 NCBI Caenorhabditis elegans C53A5.4 (C53A5.4) mRNA, complete cds. 11/11 None
SNAP00000009285 ENSEMBL cdna:SNAP chromosome:CEL140:V:14537035:14538898:-1 11/11 None
GENEFINDER00000009298 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14537035:14538898:-1 11/11 None
C53A5.4 ENSEMBL cdna:known chromosome:CEL140:V:14537035:14538898:-1 gene:C53A5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14537034-14538898(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl C53A5.4
Entrez Gene 179960 Entrez gene
SwissProt O17693 EMBL-EBI
WormBase CE08953 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03982 Hypothetical protein C53A5.4 [Caenorhabditis elegans] ref|NP_506600.1| C53A5.4 [Caenorhabditis elegans] 0.0
blast AAF99989 Hypothetical protein K06H7.8 [Caenorhabditis elegans] ref|NP_498768.1| K06H7.8 [Caenorhabditis elegans] sp|P34516|YMX8_CAEEL Putative serine/threonine-protein kinase K06H7.1 1.0E-150
blast CAE72547 Hypothetical protein CBG19731 [Caenorhabditis briggsae] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-15
Sequence
Target Sequence 
>C. ELEGANS:190509_AT
acttcatcgtgctggactttggcttggcacgccagtacatcacggataaggaggacggaa
aaaagatgcgtcgcccacgtgagaaggctctcttccgtggcacctcccgttattgctcgg
tagctatgcacgatcgtttcgagcaggggagagtcgatgacctgtgggctttgatctaca
tgctcgcggagttgagatgccagctggcttggtctgatttggatgataaggtcgaaatcg
gggaaatgaagcgacacgtcgccgaccagaatctctttgcaaaaagcccaattcaaatgc
tggagtttgtcaagatcgttcgggccacgcaattctatcaccgccccgactacgaaaagc
ttttcaatctcttgaatgatgccatgaaaagcgcgaagtacaagtggtccgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTCATCGTGCTGGACTTTGGCTT 437 103 491 Antisense
GGCACGCCAGTACATCACGGATAAG 426 535 516 Antisense
CACGTGAGAAGGCTCTCTTCCGTGG 520 199 566 Antisense
CCTCCCGTTATTGCTCGGTAGCTAT 63 267 593 Antisense
GTAGCTATGCACGATCGTTTCGAGC 341 457 610 Antisense
GAGTCGATGACCTGTGGGCTTTGAT 425 399 641 Antisense
CTTTGATCTACATGCTCGCGGAGTT 228 223 659 Antisense
ATGCCAGCTGGCTTGGTCTGATTTG 460 41 687 Antisense
CGCCGACCAGAATCTCTTTGCAAAA 576 255 750 Antisense
GGAGTTTGTCAAGATCGTTCGGGCC 84 517 792 Antisense
AGCGCGAAGTACAAGTGGTCCGACC 381 75 880 Antisense
Sequence Source Affymetrix Proprietary Database