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[190503_s_at] folate transporter

Description C50E3.11 /REP_DB=WormBase Gene ID /WP=CE08904 /TR=SW:Q22931 /GB=AAB04886.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter
Cluster 6grp_total > 99      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 5.5
(-3)
A 		12
(-2.2)
A 		7.9
(-2.7)
A 		56.7
(3)
A 		51
B 4.7
(-3)
A 		4.8
(-2.5)
A 		5.2
(-0.5)
A 		5.9
(3)
A 		1
C 4
(-1.2)
A 		7
(3)
A 		2.7
(-3)
A 		3.8
(-1.5)
A 		4
avr 4.7
(-3) 		7.9
(-1.9) 		5.3
(-2.8) 		22.1
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 13
(-2.6)
A 		10.3
(-2.7)
A 		5.8
(-3)
A 		5.9
(-3)
A 		92.8
(1.9)
A 		111.9
(3)
P 		106
B 3.2
(-3)
A 		7.7
(-2.8)
A 		8.2
(-2.7)
A 		6.8
(-2.8)
A 		119
(3)
A 		119
(3)
P 		116
C 5.7
(-2.9)
A 		6.2
(-2.9)
A 		4.7
(-3)
A 		7.4
(-2.8)
A 		70.1
(2.1)
A 		81.7
(3)
P 		77
avr 7.3
(-2.9) 		8.1
(-2.9) 		6.2
(-3) 		6.7
(-3) 		94
(2.4) 		104.2
(3) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 2
(-3)
A 		2.6
(-2.4)
A 		2.1
(-2.9)
A 		8.3
(3)
A 		6
B 1.5
(3)
A 		1.2
(-3)
A 		1.2
(-3)
A 		1.3
(-1)
A 		0
C 1.3
(0)
A 		2
(3)
A 		0.9
(-1.7)
A 		0.6
(-3)
A 		1
avr 1.6
(-2.4) 		1.9
(-1.5) 		1.4
(-3) 		3.4
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 2.4
(-2.8)
A 		3.7
(-2.5)
A 		1.3
(-3)
A 		1.5
(-3)
A 		28
(3)
A 		27.2
(2.8)
P 		27
B 1.1
(-3)
A 		2.6
(-2.8)
A 		2
(-2.9)
A 		1.8
(-2.9)
A 		48.3
(3)
A 		44.4
(2.5)
P 		47
C 2.1
(-2.8)
A 		1.9
(-2.8)
A 		1.2
(-3)
A 		1.5
(-2.9)
A 		22.7
(1.9)
A 		27.3
(3)
P 		26
avr 1.9
(-2.9) 		2.7
(-2.8) 		1.5
(-3) 		1.6
(-3) 		33
(3) 		33
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190503_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12047
Sequence Type Exemplar sequence
Representative Public ID C50E3.11 NCBI
Target Description C50E3.11 /REP_DB=WormBase Gene ID /WP=CE08904 /TR=SW:Q22931 /GB=AAB04886.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072563(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072563 NCBI Caenorhabditis elegans C50E3.11 (C50E3.11) mRNA, complete cds. 11/11 None
C50E3.11 ENSEMBL cdna:known chromosome:CEL140:V:7604915:7612330:-1 gene:C50E3.11 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028536 1/11 Cross Hyb Matching Probes None
SNAP00000028538 2/11 Cross Hyb Matching Probes None
GENEFINDER00000028558 1/11 Cross Hyb Matching Probes None
GENEFINDER00000028564 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7605200-7612331(-) 100.0 100.0
Public Domain and Genome References
Gene Title folate transporter
Ensembl C50E3.11
Entrez Gene 179144 Entrez gene
SwissProt Q22931 EMBL-EBI
WormBase CE08904 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5542 folic acid binding inferred from electronic annotation QuickGO AmiGO
8518 reduced folate carrier activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_504964 C50E3.11 [Caenorhabditis elegans] gb|AAB04886.1| Hypothetical protein C50E3.11 [Caenorhabditis elegans] sp|Q22931|FOLT2_CAEEL Folate-like transporter 0.0
blast CAE70448 Hypothetical protein CBG17027 [Caenorhabditis briggsae] 1.0E-169
blast CAE58079 Hypothetical protein CBG01160 [Caenorhabditis briggsae] 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504964.1 7 44-66,81-100,113-135,139-161,212-231,294-316,337-359
C50E3.11 7 44-66,81-100,113-135,139-161,212-231,294-316,337-359
Sequence
Target Sequence 
>C. ELEGANS:190503_S_AT
cttgcactcacacttgattcacggttgttcgggttattgttcggaatcaatacatttgta
gcattggctctacaatcaatacttacagcaatagtgatagatgcggagaagctggctatt
cgagcacaggtggacttccatctcaataaaaacattttattcggttcattttcagtttgt
gaattcggcagagcaatggggaatgctggaaaagggaaaagaccaacagaatataatgtc
gaagaagatgaattcgcgccacgtcatcaacgagaggtggatccggagcaagaagcacag
agacagcaagtacttggcgaccggcagcgtcgtatggacagagtggcggagccacactgg
gtgcaccgaatggagcttgaacccaccgtatcccactggctcgaccgcaaacctttaatg
tacgacttccaattcccgtgtatcctgcaaatctgcctggtccccaacgccaagaagaag
ttgatcgatttcgaaggaatttgttacatactctacggccatttgagatgtcccacagag
gagtgctttttcaaggattttcagccattcgagatttctctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGCACTCACACTTGATTCACGGT 242 219 991 Antisense
GAAGCTGGCTATTCGAGCACAGGTG 700 341 1098 Antisense
AGCACAGGTGGACTTCCATCTCAAT 390 77 1113 Antisense
GCCACACTGGGTGCACCGAATGGAG 641 277 1341 Antisense
GAATGGAGCTTGAACCCACCGTATC 239 333 1358 Antisense
GCTCGACCGCAAACCTTTAATGTAC 144 299 1389 Antisense
TAATGTACGACTTCCAATTCCCGTG 317 635 1406 Antisense
TGTATCCTGCAAATCTGCCTGGTCC 337 561 1429 Antisense
AATTTGTTACATACTCTACGGCCAT 559 173 1488 Antisense
CTACGGCCATTTGAGATGTCCCACA 9 227 1503 Antisense
TTTTCAGCCATTCGAGATTTCTCTT 167 677 1548 Antisense
Sequence Source Affymetrix Proprietary Database