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[190484_at]

Description Y45F3A.3 /REP_DB=WormBase Gene ID /WP=CE19166 /TR=Q9XWZ2 /GB=CAA21490.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Acyl-CoA dehydrogenase
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_highest_nonorm > 55      6grp_total > 74      4grp_total > 0      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1204
1 2 3 4 range
A 1216.8
(-2.5)
P 		1329.5
(-1.7)
P 		1138.8
(-3)
P 		2003
(3)
P 		864
B 902.5
(-3)
P 		977.7
(-2.6)
P 		1212.6
(-1.4)
P 		2096.3
(3)
P 		1194
C 1058.3
(-1.8)
P 		892.4
(-3)
P 		1399.8
(0.6)
P 		1749
(3)
P 		857
avr 1059.2
(-3) 		1066.5
(-3) 		1250.4
(-1.7) 		1949.4
(3) 		890

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1194
1 2 3 4 5 6 range
A 1506.6
(-0.5)
P 		1201.9
(-3)
P 		1929.4
(3)
P 		1847.7
(2.3)
P 		1812.3
(2)
P 		1805.3
(2)
P 		728
B 1098.3
(-3)
P 		1248.4
(-2.1)
P 		1882.9
(1.9)
P 		2052.3
(3)
P 		1829.6
(1.6)
P 		2059.9
(3)
P 		962
C 970.9
(-3)
P 		1332.2
(-1.2)
P 		1986.1
(2.1)
P 		2164.8
(3)
P 		1677.9
(0.6)
P 		2073.2
(2.5)
P 		1194
avr 1191.9
(-3) 		1260.8
(-2.5) 		1932.8
(2.4) 		2021.6
(3) 		1773.3
(1.2) 		1979.5
(2.7) 		830
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 443
(3)
P 		288.6
(-3)
P 		297.7
(-2.6)
P 		293.2
(-2.8)
P 		154
B 287.8
(-1.6)
P 		241.5
(-3)
P 		281
(-1.8)
P 		446.7
(3)
P 		205
C 350.3
(-0.6)
P 		256.6
(-3)
P 		487.9
(3)
P 		294.9
(-2)
P 		231
avr 360.4
(3) 		262.2
(-3) 		355.5
(2.7) 		344.9
(2.1) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 490
1 2 3 4 5 6 range
A 277.7
(-3)
P 		427.5
(0.3)
P 		420.2
(0.2)
P 		468.6
(1.3)
P 		546.9
(3)
P 		439.5
(0.6)
P 		269
B 382.2
(-3)
P 		415.5
(-2.5)
P 		457.2
(-1.8)
P 		543.6
(-0.5)
P 		743.3
(2.6)
P 		768.1
(3)
P 		386
C 359.9
(-3)
P 		406.5
(-2.2)
P 		505.5
(-0.4)
P 		435.3
(-1.6)
P 		542.9
(0.3)
P 		694
(3)
P 		334
avr 339.9
(-3) 		416.5
(-1.4) 		461
(-0.5) 		482.5
(-0.1) 		611
(2.5) 		633.9
(3) 		294
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190484_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8015
Sequence Type Exemplar sequence
Representative Public ID Y45F3A.3 NCBI
Target Description Y45F3A.3 /REP_DB=WormBase Gene ID /WP=CE19166 /TR=Q9XWZ2 /GB=CAA21490.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Acyl-CoA dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066928(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066928 NCBI Caenorhabditis elegans Y45F3A.3 (Y45F3A.3) mRNA, complete cds. 11/11 None
SNAP00000002979 ENSEMBL cdna:SNAP chromosome:CEL140:III:10562980:10571426:1 11/11 None
GENEFINDER00000002986 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10566469:10571426:1 11/11 None
Y45F3A.3.1 ENSEMBL cdna:known chromosome:CEL140:III:10566076:10571478:1 gene:Y45F3A.3 11/11 None
Y45F3A.3.3 ENSEMBL cdna:known chromosome:CEL140:III:10566135:10571478:1 gene:Y45F3A.3 11/11 None
Y45F3A.3.2 ENSEMBL cdna:known chromosome:CEL140:III:10566144:10571478:1 gene:Y45F3A.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10566158-10571422(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y45F3A.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21490 Hypothetical protein Y45F3A.3a [Caenorhabditis elegans] ref|NP_499329.1| Y45F3A.3 [Caenorhabditis elegans] 0.0
blast CAJ30236 Hypothetical protein Y45F3A.3b [Caenorhabditis elegans] 0.0
blast CAE71445 Hypothetical protein CBG18356 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-62
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 2.0E-67
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 1.0E-44
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 2.5E-4
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.2E-10
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 2.3E-38
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 2.5E-4
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 1.0E-44
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 2.5E-4
Sequence
Target Sequence 
>C. ELEGANS:190484_AT
caatgatgctccgtttgatcactccggtgctcaagctgtacgctggaaaacaggcggtcc
cgatggtttctgaaggaattgaatgttttggtggacagggatatatggaggatactggtc
taccaacgctgcttcgtgatgctcaagtaactccaatttgggaaggaactaccaatgttc
tatcacttgatgtgcttcgtgtattttctggaaaagaaaatattttgttggcttttggaa
aacgggttgagcaacttttgggaaataccaagactgaagacgagaagctgaaaaaatcga
aagaagccgtggaatcagctctaaaacaacttcaaaaacttctcgtaaaagcgagtgatt
ctgcaattcaaggtgaaactcgcatcgactcagtcgctcgtcacatcgccttcaccatcg
cccgcatttattctggagctcttctcatcgatcacgcttcggattccagtgttgccaatc
aatcggatattgaggttgcatatcgttattgctgtgagcagccactcattgatcttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGATGCTCCGTTTGATCACTCC 378 183 1241 Antisense
GTACGCTGGAAAACAGGCGGTCCCG 336 459 1278 Antisense
GCGGTCCCGATGGTTTCTGAAGGAA 45 293 1294 Antisense
GAGGATACTGGTCTACCAACGCTGC 461 401 1348 Antisense
AACTACCAATGTTCTATCACTTGAT 54 141 1407 Antisense
TGATGTGCTTCGTGTATTTTCTGGA 221 567 1428 Antisense
AGGTGAAACTCGCATCGACTCAGTC 486 57 1611 Antisense
CATCGCCCGCATTTATTCTGGAGCT 188 211 1656 Antisense
TATTCTGGAGCTCTTCTCATCGATC 85 665 1669 Antisense
TCACGCTTCGGATTCCAGTGTTGCC 36 627 1692 Antisense
GTGAGCAGCCACTCATTGATCTTCG 653 483 1754 Antisense
Sequence Source Affymetrix Proprietary Database