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[190474_s_at] initiation factor 5A

Description F54C9.1 /REP_DB=WormBase Gene ID /WP=CE02249 /TR=SW:Q20751 /GB=CAA90247.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=initiation factor 5A
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 07      6grp_total > 40      4grp_total > 09      6grp_highest_sub > 03_04      6grp_cgc > 60      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10527
1 2 3 4 range
A 15229.9
(-3)
P 		16489.6
(-2.2)
P 		17818.2
(-1.4)
P 		24931
(3)
P 		9701
B 14751.5
(-3)
P 		15969.2
(-1.9)
P 		17340.1
(-0.6)
P 		21249.3
(3)
P 		6498
C 14404.3
(-3)
P 		16214.7
(-1.6)
P 		14541.4
(-2.9)
P 		22430.4
(3)
P 		8026
avr 14795.2
(-3) 		16224.5
(-1.9) 		16566.6
(-1.7) 		22870.2
(3) 		8075

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9533
1 2 3 4 5 6 range
A 12393.7
(-0.3)
P 		14429.6
(1.8)
P 		12601.7
(-0.1)
P 		15537.9
(3)
P 		9884.8
(-3)
P 		11336.5
(-1.5)
P 		5653
B 11373.6
(0.5)
P 		12660.3
(1.5)
P 		14560.4
(3)
P 		11560
(0.6)
P 		10203.6
(-0.5)
P 		7023
(-3)
P 		7537
C 14169.1
(1.3)
P 		13102.2
(0.6)
P 		16556.1
(3)
P 		14309.7
(1.4)
P 		11941.6
(-0.2)
P 		7932.1
(-3)
P 		8624
avr 12645.5
(1) 		13397.4
(1.8) 		14572.7
(3) 		13802.5
(2.2) 		10676.7
(-1) 		8763.9
(-3) 		5809
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1966
1 2 3 4 range
A 5545.2
(3)
P 		3579.5
(-3)
P 		4658
(0.3)
P 		3649.4
(-2.8)
P 		1966
B 4704.1
(3)
P 		3944
(-3)
P 		4018.1
(-2.4)
P 		4528.2
(1.6)
P 		760
C 4768.2
(1.6)
P 		4661.8
(1.1)
P 		5068.1
(3)
P 		3782.1
(-3)
P 		1286
avr 5005.8
(3) 		4061.8
(-2.6) 		4581.4
(0.5) 		3986.6
(-3) 		1019

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2967
1 2 3 4 5 6 range
A 2284.7
(-3)
P 		5131.9
(3)
P 		2744.4
(-2)
P 		3940.8
(0.5)
P 		2982.8
(-1.5)
P 		2760.1
(-2)
P 		2847
B 3957.5
(2)
P 		4214.1
(3)
P 		3535.7
(0.4)
P 		3061.9
(-1.3)
P 		4145.6
(2.7)
P 		2618.8
(-3)
P 		1595
C 5251.9
(3)
P 		3997.9
(0.1)
P 		4213.8
(0.6)
P 		2877.7
(-2.5)
P 		3864.1
(-0.2)
P 		2655.3
(-3)
P 		2597
avr 3831.4
(0.9) 		4448
(3) 		3498
(-0.2) 		3293.5
(-0.9) 		3664.2
(0.3) 		2678.1
(-3) 		1770
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190474_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3051
Sequence Type Exemplar sequence
Representative Public ID F54C9.1 NCBI
Target Description F54C9.1 /REP_DB=WormBase Gene ID /WP=CE02249 /TR=SW:Q20751 /GB=CAA90247.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=initiation factor 5A
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063406(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063406 NCBI Caenorhabditis elegans Initiation Factor Five (eIF-5A) homologs family member (iff-2) (iff-2) mRNA, complete cds. 11/11 None
SNAP00000013415 ENSEMBL cdna:SNAP chromosome:CEL140:II:8559730:8560877:-1 11/11 None
GENEFINDER00000013450 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8559730:8560877:-1 11/11 None
F54C9.1.3 ENSEMBL cdna:known chromosome:CEL140:II:8559333:8560888:-1 gene:F54C9.1 11/11 None
F54C9.1.1 ENSEMBL cdna:known chromosome:CEL140:II:8559333:8561242:-1 gene:F54C9.1 11/11 None
F54C9.1.2 ENSEMBL cdna:known chromosome:CEL140:II:8559333:8560886:-1 gene:F54C9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8559726-8560874(-) 100.0 100.0
Public Domain and Genome References
Gene Title initiation factor 5A
Gene Symbol iff-2
Ensembl F54C9.1
Entrez Gene 174367 Entrez gene
SwissProt Q20751 EMBL-EBI
WormBase CE02249 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262615_AT eukaryotic translation initiation factor 5A-1 / eIF-5A 1 at
ATGENOME1:13072_AT eukaryotic translation initiation factor 5A-1 / eIF-5A 1 at
HUGENEFL:S72024_S_AT similar to Eukaryotic translation initiation factor 5A (eIF-5A) (eIF-4D) (Rev-binding factor) hs
YEAST_2:1779407_AT Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6413 translational initiation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3743 translation initiation factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90247 Hypothetical protein F54C9.1 [Caenorhabditis elegans] ref|NP_495807.1| Initiation Factor Five (eIF-5A) homologs family member (iff-2) [Caenorhabditis elegans] sp|Q20751|IF5A2_CAEEL Eukaryotic translation initiation factor 5A-2 (eIF-5A-2) (Initiation factor five protein 2) 1.0E-90
blast CAE57587 Hypothetical protein CBG00567 [Caenorhabditis briggsae] 3.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001884 EMBL-EBI Eukaryotic initiation factor 5A hypusine (eIF-5A) 1.8E-33
Pfam IPR005824 EMBL-EBI KOW 2.9E-8
Sequence
Target Sequence 
>C. ELEGANS:190474_S_AT
ccacattcccaaagcagtgctctgctctccgtaagaacgagcatgtcatgatcaagggac
gcccatgcaagatcgtcgaaatgtcgacctccaagaccggaaagcacggtcacgctaagg
tccacatggtcgccatcgacatcttcaccagcaagaagcttgaagatatctgtccatcca
cccacaacatggatgttccagtcgtcaagcgtcgtgagtacttgttgatggccatcgatg
acggatattgctccctcatggacccagagtcctgcgagcaaaaggatgacctcaagcttc
ccgatactgagctcggacaacagatccgtgatgcttatgagaaggacgagggatccgttt
tggtccaagttgtctctgctatcggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACATTCCCAAAGCAGTGCTCTGC 510 275 71 Antisense
GTCGACCTCCAAGACCGGAAAGCAC 335 465 153 Antisense
TCACGCTAAGGTCCACATGGTCGCC 35 627 180 Antisense
CTTGAAGATATCTGTCCATCCACCC 657 217 229 Antisense
TCCAGTCGTCAAGCGTCGTGAGTAC 347 589 267 Antisense
GTGAGTACTTGTTGATGGCCATCGA 171 483 284 Antisense
ATCGATGACGGATATTGCTCCCTCA 151 35 304 Antisense
CTCATGGACCCAGAGTCCTGCGAGC 517 229 325 Antisense
ACCTCAAGCTTCCCGATACTGAGCT 9 87 359 Antisense
GAGGGATCCGTTTTGGTCCAAGTTG 333 401 418 Antisense
GTCCAAGTTGTCTCTGCTATCGGAG 257 475 433 Antisense
Sequence Source Affymetrix Proprietary Database