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[190469_at] troponin I

Description ZK721.2 /REP_DB=WormBase Gene ID /WP=CE05106 /GEN=unc-27 /TR=Q9GYF1 /GB=AAG01564.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=troponin I
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 71      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 28      6grp_total > 72      4grp_total > 59      6grp_highest_sub > 03_04      6grp_cgc > 75      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9476
1 2 3 4 range
A 6228.9
(-0.1)
P 		9078.6
(1.7)
P 		11054.9
(3)
P 		1579.4
(-3)
P 		9476
B 6038.1
(-0.2)
P 		9360.6
(2)
P 		10916.9
(3)
P 		1751.6
(-3)
P 		9165
C 5933.7
(-0.1)
P 		9824.5
(2.5)
P 		10636.6
(3)
P 		1593
(-3)
P 		9044
avr 6066.9
(-0.1) 		9421.2
(2.1) 		10869.5
(3) 		1641.3
(-3) 		9228

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8793
1 2 3 4 5 6 range
A 6729.9
(-3)
P 		10160.6
(-0.6)
P 		11503
(0.3)
P 		15479.1
(3)
P 		10336.5
(-0.5)
P 		8578.6
(-1.7)
P 		8749
B 6686.5
(-3)
P 		8906.5
(0.1)
P 		10652.4
(2.5)
P 		11024.8
(3)
P 		9971.4
(1.5)
P 		6880.9
(-2.7)
P 		4338
C 8038.3
(-2.3)
P 		8584.5
(-1.9)
P 		15408.9
(3)
P 		13805.7
(1.8)
P 		12787.7
(1.1)
P 		7077.1
(-3)
P 		8332
avr 7151.6
(-3) 		9217.2
(-1) 		12521.4
(2.1) 		13436.5
(3) 		11031.9
(0.7) 		7512.2
(-2.7) 		6285
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3476
1 2 3 4 range
A 2267.9
(1.6)
P 		1970.7
(0.9)
P 		2890
(3)
P 		231.2
(-3)
P 		2659
B 1925.5
(1.3)
P 		2311.8
(2.4)
P 		2529.7
(3)
P 		373.3
(-3)
P 		2156
C 1964.2
(-0)
P 		2824.6
(1.5)
P 		3707.2
(3)
P 		268.6
(-3)
P 		3439
avr 2052.5
(0.8) 		2369
(1.5) 		3042.3
(3) 		291
(-3) 		2751

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2897
1 2 3 4 5 6 range
A 1240.6
(-3)
P 		3613.6
(2.3)
P 		2505.1
(-0.2)
P 		3925.9
(3)
P 		3119.1
(1.2)
P 		2088.6
(-1.1)
P 		2685
B 2326.6
(-3)
P 		2964.6
(-0.8)
P 		2586.7
(-2.1)
P 		2920.1
(-0.9)
P 		4051.2
(3)
P 		2565.9
(-2.2)
P 		1725
C 2979.4
(-0.9)
P 		2619.4
(-2.2)
P 		3921.8
(2.3)
P 		2776.3
(-1.6)
P 		4137.9
(3)
P 		2369.1
(-3)
P 		1769
avr 2182.2
(-3) 		3065.9
(0.3) 		3004.5
(0.1) 		3207.4
(0.9) 		3769.4
(3) 		2341.2
(-2.4) 		1587
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190469_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17224
Sequence Type Exemplar sequence
Representative Public ID ZK721.2 NCBI
Target Description ZK721.2 /REP_DB=WormBase Gene ID /WP=CE05106 /GEN=unc-27 /TR=Q9GYF1 /GB=AAG01564.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=troponin I
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077087(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077087 NCBI Caenorhabditis elegans UNCoordinated family member (unc-27) (unc-27) mRNA, complete cds. 11/11 None
GENEFINDER00000024785 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8788756:8790292:1 11/11 None
SNAP00000024778 ENSEMBL cdna:SNAP chromosome:CEL140:X:8789393:8790292:1 11/11 None
ZK721.2 ENSEMBL cdna:known chromosome:CEL140:X:8787930:8790558:1 gene:ZK721.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8787928-8790353(+) 96.53 100.0
Public Domain and Genome References
Gene Title troponin I
Gene Symbol unc-27
Ensembl ZK721.2
Entrez Gene 181124 Entrez gene
SwissProt Q9GYF1 EMBL-EBI
WormBase CE05106 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG01564 Uncoordinated protein 27 [Caenorhabditis elegans] ref|NP_509488.1| UNCoordinated family member (unc-27) [Caenorhabditis elegans] 1.0E-114
blast CAE69357 Hypothetical protein CBG15446 [Caenorhabditis briggsae] 4.0E-78
blast AAG01564 Uncoordinated protein 27 [Caenorhabditis elegans] ref|NP_509488.1| UNCoordinated family member (unc-27) [Caenorhabditis elegans] 3.0E-75
blast BAD89378.1 troponin I 2 [Caenorhabditis elegans] 3.0E-75
blast AAG01564 Uncoordinated protein 27 [Caenorhabditis elegans] ref|NP_509488.1| UNCoordinated family member (unc-27) [Caenorhabditis elegans] 4.0E-75
blast BAD89378.1 troponin I 2 [Caenorhabditis elegans] 4.0E-75
blast BAD89378.1 troponin I 2 [Caenorhabditis elegans] 5.0E-75
blast CAE69357 Hypothetical protein CBG15446 [Caenorhabditis briggsae] 2.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR001978 EMBL-EBI Troponin 1.7E-40
Pfam IPR001978 EMBL-EBI Troponin 1.7E-40
Pfam IPR001978 EMBL-EBI Troponin 1.7E-40
Sequence
Target Sequence 
>C. ELEGANS:190469_AT
gaagggattcttgacaccagagcgtaagaagaagcttcgtaagcttttgatggtcaaggc
cgccgaggatcttaagagacaacaattgcttaaggagcaagaaagacaaaaggctcttgc
tgacagaaccatctcacttccaaatgttgactccattgatgataagggacaactcgagaa
gatctacaatgacctctgggctcgtcttacccagctcgaggaggagaagtacgacatcaa
ctacgtcgtctcccagactgaggctgagatcaactccctcactattgaggtcaacgatct
tcgtggaaagttcgtcaagccttcactcaagaaggtgtcgaagtacgacaacaagttcaa
gaagagcggtgagagcaaggccggaaccaaggaagacttccgtgccaacctcaagattgt
caagaaggacgttatggaggccatcgtcaatgttaagaagaaggacgacaagccagattg
gtccaagaagaacaaggatgccaaggccgaagactccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGGATTCTTGACACCAGAGCGT 573 335 315 Antisense
TGATGGTCAAGGCCGCCGAGGATCT 71 567 362 Antisense
TGACAGAACCATCTCACTTCCAAAT 680 569 435 Antisense
AAGATCTACAATGACCTCTGGGCTC 379 155 493 Antisense
CTCGTCTTACCCAGCTCGAGGAGGA 619 231 515 Antisense
TCGTCTCCCAGACTGAGGCTGAGAT 249 605 560 Antisense
CTGAGATCAACTCCCTCACTATTGA 89 239 578 Antisense
TGGAAAGTTCGTCAAGCCTTCACTC 90 547 618 Antisense
GAGCGGTGAGAGCAAGGCCGGAACC 242 385 678 Antisense
TTCCGTGCCAACCTCAAGATTGTCA 561 699 712 Antisense
AGGATGCCAAGGCCGAAGACTCCGC 109 57 809 Antisense
Sequence Source Affymetrix Proprietary Database