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[190445_at] cysteine synthase like

Description F01D4.8 /REP_DB=WormBase Gene ID /WP=CE09160 /TR=O01982 /GB=CAA94593.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=cysteine synthase like
Cluster 6grp_total > 78      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3260
1 2 3 4 range
A 186.3
(-2.8)
P 		99
(-3)
A 		123.8
(-3)
A 		3358.6
(3)
P 		3260
B 194.2
(-2.8)
P 		117.3
(-3)
A 		127
(-3)
A 		2909.2
(3)
P 		2792
C 204
(-2.7)
P 		103.7
(-3)
A 		117.9
(-3)
A 		2504.2
(3)
P 		2401
avr 194.8
(-2.8) 		106.7
(-3) 		122.9
(-3) 		2924
(3) 		2817

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1915
1 2 3 4 5 6 range
A 138.9
(-2.8)
A 		150.2
(-2.7)
P 		73.4
(-3)
A 		64.7
(-3)
A 		770
(-0.7)
P 		1874.5
(3)
P 		1810
B 192.6
(-2.6)
P 		139
(-2.8)
P 		65.9
(-3)
A 		90.4
(-2.9)
A 		886.3
(-0.3)
P 		1919.5
(3)
P 		1854
C 122.5
(-2.6)
P 		175.7
(-2.4)
P 		4.1
(-3)
A 		143.8
(-2.6)
A 		680.1
(-0.8)
P 		1867.4
(3)
P 		1863
avr 151.3
(-2.7) 		155
(-2.7) 		47.8
(-3) 		99.6
(-2.8) 		778.8
(-0.6) 		1887.1
(3) 		1839
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 599
1 2 3 4 range
A 67.8
(-2.4)
P 		21.5
(-3)
A 		32.4
(-2.9)
A 		491.6
(3)
P 		470
B 61.9
(-2.7)
P 		29
(-3)
A 		29.4
(-3)
A 		620
(3)
P 		591
C 67.5
(-2.4)
P 		29.8
(-3)
A 		41.1
(-2.8)
A 		422.2
(3)
P 		392
avr 65.7
(-2.5) 		26.8
(-3) 		34.3
(-2.9) 		511.3
(3) 		485

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 715
1 2 3 4 5 6 range
A 25.6
(-2.9)
A 		53.4
(-2.5)
P 		16
(-3)
A 		16.4
(-3)
A 		232.3
(-0.1)
P 		456.4
(3)
P 		440
B 67
(-2.6)
P 		46.3
(-2.7)
P 		16
(-3)
A 		23.9
(-2.9)
A 		360.1
(-0)
P 		715.8
(3)
P 		700
C 45.4
(-2.6)
P 		53.6
(-2.5)
P 		1
(-3)
A 		28.9
(-2.7)
A 		220.1
(-0.9)
P 		625.1
(3)
P 		624
avr 46
(-2.6) 		51.1
(-2.6) 		11
(-3) 		23.1
(-2.9) 		270.8
(-0.3) 		599.1
(3) 		588
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190445_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9509
Sequence Type Exemplar sequence
Representative Public ID F01D4.8 NCBI
Target Description F01D4.8 /REP_DB=WormBase Gene ID /WP=CE09160 /TR=O01982 /GB=CAA94593.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=cysteine synthase like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069528(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069528 NCBI Caenorhabditis elegans F01D4.8 (F01D4.8) mRNA, complete cds. 11/11 None
SNAP00000009881 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10450780:10451640:-1 11/11 None
GENEFINDER00000009887 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10450780:10451640:-1 11/11 None
F01D4.8 ENSEMBL cdna:known chromosome:CEL140:IV:10450780:10453070:-1 gene:F01D4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10450785-10453076(-) 100.0 100.0
Public Domain and Genome References
Gene Title cysteine synthase like
Ensembl F01D4.8
Entrez Gene 184055 Entrez gene
SwissProt O01982 EMBL-EBI
WormBase CE09160 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16829 lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02888 Hypothetical protein F01D4.8 [Caenorhabditis elegans] emb|CAA94593.1| Hypothetical protein F01D4.8 [Caenorhabditis elegans] ref|NP_501929.1| F01D4.8 [Caenorhabditis elegans] 0.0
blast CAA94589 Hypothetical protein F13B12.4 [Caenorhabditis elegans] ref|NP_501928.1| F13B12.4 [Caenorhabditis elegans] 1.0E-176
blast CAB02888 Hypothetical protein F01D4.8 [Caenorhabditis elegans] emb|CAA94593.1| Hypothetical protein F01D4.8 [Caenorhabditis elegans] ref|NP_501929.1| F01D4.8 [Caenorhabditis elegans] 1.0E-156
blast CAE63818 Hypothetical protein CBG08368 [Caenorhabditis briggsae] 1.0E-131
blast CAA94589 Hypothetical protein F13B12.4 [Caenorhabditis elegans] ref|NP_501928.1| F13B12.4 [Caenorhabditis elegans] 1.0E-106
Protein Domains
Database ID Description E-Value
scop a.4.3.ARID domain All alpha proteins; DNA/RNA-binding 3-helical bundle; ARID-like; ARID domain 6.80000019073486
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.3E-44
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 2.8E-17
Sequence
Target Sequence 
>C. ELEGANS:190445_AT
ggtacctgatttctttgttcatcctgctggcactggtggaactgtatcatcggttggaag
atatgtagtcaaatatggtttacctacaaaagttgttcttgctgattcacaattttcggt
ttactatgattatgtaattggaaacaagttcacaaatcaaactggagaaaaagtctggag
agctccaggaattgctggaacaggatatggatatgatattgtaccaatacagtatggaga
aacaacaagtttaacaaaaaatgtaataaatgaagctatgaagatgcctgatattgcaac
agttgccgccatgagaattcttaatgaaatgggatataatgccggtgcatcaacatctct
caattttttggtttgcctttacaaagcatatcaaacaaaacaaaaatcatccaacaatga
atatcagcttacaattgcaactttggctggtgatcccggagatttttatcatagcactta
cctagatgacggatgggttgatacacatttaaattatgttggcggtgtgactgcaatgga
atgttggaagaagttaatacaagaatctatcgatacgggatccaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTACCTGATTTCTTTGTTCATCCT 267 505 687 Antisense
TTCATCCTGCTGGCACTGGTGGAAC 100 687 704 Antisense
GGCACTGGTGGAACTGTATCATCGG 639 535 715 Antisense
GTTCTTGCTGATTCACAATTTTCGG 599 443 781 Antisense
GATGCCTGATATTGCAACAGTTGCC 513 415 969 Antisense
ACAGTTGCCGCCATGAGAATTCTTA 565 107 985 Antisense
TAATGCCGGTGCATCAACATCTCTC 230 637 1023 Antisense
ATCTCTCAATTTTTTGGTTTGCCTT 477 29 1041 Antisense
AACTTTGGCTGGTGATCCCGGAGAT 634 139 1125 Antisense
ATGTTGGCGGTGTGACTGCAATGGA 650 45 1202 Antisense
GAATCTATCGATACGGGATCCAATT 639 327 1249 Antisense
Sequence Source Affymetrix Proprietary Database