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[190428_s_at]

Description F15D3.1A /REP_DB=WormBase Gene ID /WP=CE27129 /GEN=dys-1 /TR=Q9TW65 /GB=CAB61005.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=dystrophin
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_high_8_8 > 34      6grp_highest_nonorm > 50      6grp_total > 25      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 497
1 2 3 4 range
A 687.7
(3)
P 		646.9
(2.3)
P 		346.3
(-3)
P 		358.8
(-2.8)
A 		341
B 761.9
(3)
P 		555.5
(-0.1)
P 		397.8
(-2.4)
P 		360.1
(-3)
P 		402
C 629.3
(3)
P 		456.9
(0.2)
P 		360.8
(-1.4)
P 		265.2
(-3)
P 		364
avr 693
(3) 		553.1
(0.7) 		368.3
(-2.3) 		328
(-3) 		365

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 538
1 2 3 4 5 6 range
A 729.1
(3)
P 		635.9
(1.8)
P 		372.2
(-1.5)
P 		249.8
(-3)
P 		255.4
(-2.9)
P 		277.1
(-2.7)
P 		479
B 666.1
(3)
P 		604.8
(2.2)
P 		509.6
(1)
P 		360.4
(-1)
P 		204.6
(-3)
P 		246
(-2.5)
P 		462
C 686.3
(3)
P 		537.1
(1.2)
P 		386.2
(-0.6)
P 		239.6
(-2.4)
P 		258.8
(-2.2)
P 		191
(-3)
P 		495
avr 693.8
(3) 		592.6
(1.7) 		422.7
(-0.6) 		283.3
(-2.4) 		239.6
(-3) 		238
(-3) 		456
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 250.4
(3)
P 		140.4
(-0.3)
P 		90.5
(-1.8)
P 		52.5
(-3)
A 		198
B 243
(3)
P 		137.2
(-0.8)
P 		92.2
(-2.4)
P 		76.7
(-3)
P 		166
C 208.3
(3)
P 		131.4
(0.2)
P 		125.8
(-0)
P 		44.7
(-3)
P 		164
avr 233.9
(3) 		136.3
(-0.3) 		102.8
(-1.5) 		58
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 134.4
(-0.4)
P 		226.2
(3)
P 		81.1
(-2.3)
P 		63.3
(-3)
P 		77.1
(-2.5)
P 		67.5
(-2.8)
P 		163
B 231.8
(3)
P 		201.3
(1.8)
P 		123.7
(-1.4)
P 		95.5
(-2.5)
P 		83.1
(-3)
P 		91.7
(-2.7)
P 		149
C 254.4
(3)
P 		163.9
(0.4)
P 		98.3
(-1.5)
P 		48.2
(-3)
P 		83.8
(-2)
P 		63.9
(-2.5)
P 		206
avr 206.9
(3) 		197.1
(2.6) 		101
(-1.6) 		69
(-3) 		81.3
(-2.5) 		74.4
(-2.8) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190428_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2262
Sequence Type Exemplar sequence
Representative Public ID F15D3.1A NCBI
Target Description F15D3.1A /REP_DB=WormBase Gene ID /WP=CE27129 /GEN=dys-1 /TR=Q9TW65 /GB=CAB61005.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=dystrophin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060546(11), NM_060545(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060546 NCBI Caenorhabditis elegans F15D3.1b (dystrophin) mRNA, complete cds. 11/11 None
NM_060545 NCBI Caenorhabditis elegans F15D3.1a (dystrophin) mRNA, complete cds. 11/11 None
F15D3.1b ENSEMBL cdna:known chromosome:CEL140:I:11534855:11537924:-1 gene:F15D3.1 11/11 None
F15D3.1a ENSEMBL cdna:known chromosome:CEL140:I:11535199:11565914:-1 gene:F15D3.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11535129-11565845(-) 99.6 100.0
Public Domain and Genome References
Ensembl F15D3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61012 Hypothetical protein F15D3.1a [Caenorhabditis elegans] emb|CAB61005.2| Hypothetical protein F15D3.1a [Caenorhabditis elegans] ref|NP_492946.1| F15D3.1a [Caenorhabditis elegans] sp|Q9TW65|DMD_CAEEL Dystrophin-1 0.0
blast CAA10033 DYS-1 protein [Caenorhabditis elegans] 0.0
blast T20978 hypothetical protein F15D3.9 - Caenorhabditis elegans 0.0
blast CAB61004 Hypothetical protein F15D3.1b [Caenorhabditis elegans] ref|NP_492947.1| F15D3.1b [Caenorhabditis elegans] 1.0E-105
blast T21636 hypothetical protein F32B4.3a - Caenorhabditis elegans 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 9.6E-5
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.1E-22
Pfam IPR000433 EMBL-EBI Zn-finger, ZZ type 9.6E-16
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.5E-10
Pfam IPR002017 EMBL-EBI Spectrin repeat 3.9E-6
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.0024
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.096
Pfam IPR002017 EMBL-EBI Spectrin repeat 5.6E-8
Pfam IPR002017 EMBL-EBI Spectrin repeat 0.083
Sequence
Target Sequence 
>C. ELEGANS:190428_S_AT
gagtcggggaggaacgttaccattgagaaatggtagaagtgtagtgtcattgaagagcac
acagagccagaatgacgtaatggacgaggcgaaagccctacgcctccacaagcaacgttt
ggagcatcgctctcgaattctagagcagcaaaatgagcagctcgagatgcagctgcaaag
gttgaagaaagttatagatgcgcaaaagcaacaagctcccctctcaaccaactcgttgct
ccgcggaagtcatcatcaaccatggagccccgaacgtgctcgttcgggctccgcgagcac
actggatcgtggtcttattgtgagtagtcgtcatcaggagcaggcagaagcggccggcgg
cggagctgaagactcatcggatgaagctggcggagccggtggtgggccacgtggcagcag
tgtcggacaaatgcaaaatctaatgacagcttgtgatgatttaggaaaagcaatggaatc
cctggtggtgtctgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCGGGGAGGAACGTTACCATTG 140 1 10476 Antisense
GACGAGGCGAAAGCCCTACGCCTCC 467 379 10558 Antisense
TACGCCTCCACAAGCAACGTTTGGA 471 649 10574 Antisense
TGGAGCATCGCTCTCGAATTCTAGA 435 549 10595 Antisense
ATGCGCAAAAGCAACAAGCTCCCCT 604 41 10673 Antisense
GTTGCTCCGCGGAAGTCATCATCAA 62 441 10710 Antisense
GAGCACACTGGATCGTGGTCTTATT 228 391 10770 Antisense
GAGTAGTCGTCATCAGGAGCAGGCA 199 399 10797 Antisense
GCGGCGGAGCTGAAGACTCATCGGA 690 293 10832 Antisense
CCACGTGGCAGCAGTGTCGGACAAA 265 275 10882 Antisense
ATGGAATCCCTGGTGGTGTCTGTTG 341 53 10948 Antisense
Sequence Source Affymetrix Proprietary Database