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[190426_at] calpain thiol protease

Description W04A4.4 /REP_DB=WormBase Gene ID /WP=CE14574 /TR=O18165 /GB=CAB04898.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain thiol protease
Cluster 6grp_total > 33      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 10.2
(-2.2)
A
4.6
(-3)
A
12.3
(-1.8)
A
44.4
(3)
A
40
B 22.2
(0.3)
A
16.1
(-1.3)
A
9.2
(-3)
A
32.9
(3)
A
24
C 16.7
(-2.6)
A
12.8
(-3)
A
12.6
(-3)
A
80.5
(3)
P
68
avr 16.4
(-2.2)
11.2
(-3)
11.4
(-3)
52.6
(3)
41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 26.7
(0.8)
A
19.3
(-0.3)
A
41.4
(3)
M
1.5
(-3)
A
32.8
(1.7)
A
7.9
(-2)
A
40
B 22.7
(1.6)
A
23.2
(2.1)
P
23.9
(2.8)
A
18.6
(-2.5)
A
24.1
(3)
A
18.1
(-3)
A
6
C 21.1
(-0.1)
A
42.4
(3)
P
23.9
(0.3)
A
18.6
(-0.4)
A
2.8
(-2.7)
A
0.7
(-3)
A
42
avr 23.5
(1.2)
28.3
(2.6)
29.7
(3)
12.9
(-1.8)
19.9
(0.2)
8.9
(-3)
21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 3.7
(-0.1)
A
1
(-3)
A
3.2
(-0.6)
A
6.5
(3)
A
6
B 7.1
(3)
A
4
(-0.7)
A
2.1
(-3)
A
7
(2.9)
A
5
C 5.5
(-1.9)
A
3.7
(-3)
A
4.4
(-2.6)
A
13.6
(3)
P
10
avr 5.4
(-0.5)
2.9
(-3)
3.2
(-2.7)
9
(3)
6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 4.9
(-0.2)
A
6.9
(1.1)
A
9
(2.4)
M
0.4
(-3)
A
9.9
(3)
A
1.9
(-2.1)
A
10
B 7.9
(0.7)
A
7.7
(0.4)
P
5.8
(-1.9)
A
4.9
(-3)
A
9.8
(3)
A
6.7
(-0.8)
A
5
C 7.8
(0.6)
A
13
(3)
P
6.1
(-0.2)
A
3.7
(-1.4)
A
0.9
(-2.7)
A
0.2
(-3)
A
13
avr 6.9
(0.8)
9.2
(3)
7
(0.9)
3
(-2.9)
6.9
(0.8)
2.9
(-3)
6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190426_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2434
Sequence Type Exemplar sequence
Representative Public ID W04A4.4 NCBI
Target Description W04A4.4 /REP_DB=WormBase Gene ID /WP=CE14574 /TR=O18165 /GB=CAB04898.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain thiol protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060996(10)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_060996 NCBI Caenorhabditis elegans W04A4.4 (W04A4.4) mRNA, complete cds. 10/11 None
GENEFINDER00000016152 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13684373:13685508:-1 10/11 None
W04A4.4 ENSEMBL cdna:known chromosome:CEL140:I:13684373:13685508:-1 gene:W04A4.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016125 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13684303-13685439(-) 100.0 100.0
Public Domain and Genome References
Gene Title calpain thiol protease
Ensembl W04A4.4
Entrez Gene 189180 Entrez gene
SwissProt O18165 EMBL-EBI
WormBase CE36213 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04898 Hypothetical protein W04A4.4 [Caenorhabditis elegans] ref|NP_493397.2| W04A4.4 [Caenorhabditis elegans] 1.0E-126
blast AAT48617 Hypothetical protein F47F6.9 [Caenorhabditis elegans] ref|NP_001022179.1| F47F6.9 [Caenorhabditis elegans] 8.0E-34
blast C88040 protein F47F6.5 [imported] - Caenorhabditis elegans 8.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.2E-5
Sequence
Target Sequence
>C. ELEGANS:190426_AT
gaaaatcgactttcatttgccacagaaatcgaattcatttgaaagatatgcatatatggt
gaagaaacaaatatgggttgcattcattgaaaaaggatttgctaaaattcgaaaaagcta
tgagaaactaagtggcggtgttgcaggcatagcgcttcaacagctaacaggtgctatgac
attttcggtctttatggagaaattcaataatgatgaaaatcgtgtgtgggagtttattca
ggaaaatcgaaactccaagtttatcctgacggttagcacaccaacaattgaagaagaatc
tgaaaaaaaacaacttctcgaagaatatggtattcgtgattgtcatgagtattctgtttt
ggatgctcaagtatacatgggccaccgcctaatcttacttgcgggttctggaccgtttga
aatacgtgaagattggtgtgcggtggacttgggattttcggaatttggaactttttggat
tgatatgagtgagctctttcaatact


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GAAAATCGACTTTCATTTGCCACAG 708 353 69 Antisense
GGCGGTGTTGCAGGCATAGCGCTTC 441 541 202 Antisense
AGCGCTTCAACAGCTAACAGGTGCT 589 81 219 Antisense
GGTGCTATGACATTTTCGGTCTTTA 66 501 238 Antisense
GAAACTCCAAGTTTATCCTGACGGT 609 357 317 Antisense
TATCCTGACGGTTAGCACACCAACA 625 655 330 Antisense
TGCTCAAGTATACATGGGCCACCGC 622 577 432 Antisense
GCCACCGCCTAATCTTACTTGCGGG 572 277 449 Antisense
TACTTGCGGGTTCTGGACCGTTTGA 206 643 464 Antisense
GTGCGGTGGACTTGGGATTTTCGGA 153 471 506 Antisense
GATATGAGTGAGCTCTTTCAATACT 179 425 550 Antisense
Sequence Source Affymetrix Proprietary Database