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[190424_at]

Description C46C2.1 /REP_DB=WormBase Gene ID /WP=CE03080 /TR=Q18657 /GB=CAA92591.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 56      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 85      6grp_total > 46      4grp_total > 23      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 828
1 2 3 4 range
A 1236
(3)
P 		528.3
(-3)
P 		642.3
(-2)
P 		1110.4
(1.9)
P 		708
B 1356.7
(3)
P 		647
(-3)
P 		702.8
(-2.5)
P 		1256.1
(2.1)
P 		710
C 1169.7
(3)
P 		615.4
(-3)
P 		733.2
(-1.7)
P 		1018.7
(1.4)
P 		554
avr 1254.1
(3) 		596.9
(-3) 		692.8
(-2.1) 		1128.4
(1.9) 		657

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 643
1 2 3 4 5 6 range
A 879.2
(1.4)
P 		953.6
(2.4)
P 		536.3
(-3)
P 		561.1
(-2.7)
P 		595.5
(-2.2)
P 		1002
(3)
P 		466
B 870.7
(2.1)
P 		761.4
(1)
P 		687.5
(0.3)
P 		761.8
(1)
P 		359.5
(-3)
P 		958.8
(3)
P 		599
C 831.7
(0.7)
P 		676.1
(-1.5)
P 		995.9
(3)
P 		571.1
(-3)
P 		611.5
(-2.4)
P 		971.6
(2.7)
P 		425
avr 860.5
(1.5) 		797
(0.6) 		739.9
(-0.1) 		631.3
(-1.6) 		522.2
(-3) 		977.5
(3) 		455
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 335
1 2 3 4 range
A 450
(3)
P 		114.7
(-3)
P 		167.9
(-2)
P 		162.5
(-2.1)
P 		335
B 432.7
(3)
P 		159.8
(-3)
P 		162.9
(-2.9)
P 		267.7
(-0.6)
P 		273
C 387.2
(3)
P 		176.9
(-2.9)
P 		255.5
(-0.7)
P 		171.8
(-3)
P 		215
avr 423.3
(3) 		150.5
(-3) 		195.4
(-2) 		200.7
(-1.9) 		273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 243
1 2 3 4 5 6 range
A 162.1
(-1.8)
P 		339.1
(3)
P 		116.8
(-3)
P 		142.3
(-2.3)
P 		179.7
(-1.3)
P 		244
(0.4)
P 		222
B 302.9
(1.5)
P 		253.4
(0)
P 		166.9
(-2.4)
P 		201.8
(-1.4)
P 		146.1
(-3)
P 		357.5
(3)
P 		211
C 308.3
(2.5)
P 		206.3
(-0.4)
P 		253.5
(1)
P 		114.8
(-3)
P 		197.9
(-0.6)
P 		325.3
(3)
P 		211
avr 257.8
(1) 		266.3
(1.4) 		179.1
(-2) 		153
(-3) 		174.6
(-2.2) 		308.9
(3) 		156
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10096
Sequence Type Exemplar sequence
Representative Public ID C46C2.1 NCBI
Target Description C46C2.1 /REP_DB=WormBase Gene ID /WP=CE03080 /TR=Q18657 /GB=CAA92591.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182275(10), NM_069202(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182275 NCBI Caenorhabditis elegans mammalian WNK-type protein kinase homolog family member (wnk-1) (wnk-1) mRNA, complete cds. 10/11 None
NM_069202 NCBI Caenorhabditis elegans mammalian WNK-type protein kinase homolog family member (wnk-1) (wnk-1) mRNA, complete cds. 11/11 None
SNAP00000024423 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9210348:9211572:1 9/11 None
GENEFINDER00000024428 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9203272:9211558:1 10/11 None
C46C2.1b ENSEMBL cdna:known chromosome:CEL140:IV:9200094:9212200:1 gene:C46C2.1 10/11 A 
C46C2.1a ENSEMBL cdna:known chromosome:CEL140:IV:9200409:9211634:1 gene:C46C2.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024422 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9201297-9211564(+) 98.9 100.0
Public Domain and Genome References
Ensembl C46C2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD59142 Hypothetical protein C46C2.1b [Caenorhabditis elegans] ref|NP_872075.2| mammalian WNK-type protein kinase homolog family member (wnk-1) [Caenorhabditis elegans] 0.0
blast CAA92591 Hypothetical protein C46C2.1a [Caenorhabditis elegans] ref|NP_501603.3| mammalian WNK-type protein kinase homolog family member (wnk-1) [Caenorhabditis elegans] 0.0
blast CAE74131 Hypothetical protein CBG21799 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.5 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DmCamKII 2.0E-64
hanks 2.1.5 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DmCamKII 1.0E-65
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.59999990463257
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-70
Pfam IPR000719 EMBL-EBI Protein kinase 6.8E-67
Sequence
Target Sequence 
>C. ELEGANS:190424_AT
ccgttccctgtttcggagtgtgattatgatttgaagggtcaaatggatttggaaagcgaa
gatcctgaggtgatccagatgattgttcgtcatcgaatggaacaacacaaacttctcgaa
aagcaacgtgtcgagattgagcgtcttcgttcgaaaattcgagttccacgtgcaacttct
gtgaatcccgaaatgattggagatgatgaagctgatacgacgcttactgctcttcaaagt
gcattaggcaatgcttcattgtcattgccagccagtccgccacctaatactgagacaacg
aaggtcaacaccactgtaattccttccgatgtattggcgacccgaatgacaatgtctcaa
tcgtcaaccaaatcaagcaacgtctcggtatcatcccgtcatcgcgacaatcagtccgct
ccgccacgtcatcatcaccatcaaccccaccctcctcatcatccgcacctccaaaatcac
tatcatcccccacaaaatcatacgtcagccactgctccttgtccatcggctatggttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTTCCCTGTTTCGGAGTGTGATT 480 259 4945 Antisense
GTGTCGAGATTGAGCGTCTTCGTTC 645 485 5072 Antisense
GTTCCACGTGCAACTTCTGTGAATC 118 441 5107 Antisense
AGCTGATACGACGCTTACTGCTCTT 292 81 5154 Antisense
TGCTTCATTGTCATTGCCAGCCAGT 700 579 5196 Antisense
CCAGTCCGCCACCTAATACTGAGAC 520 271 5216 Antisense
ACCACTGTAATTCCTTCCGATGTAT 534 85 5254 Antisense
TCCGATGTATTGGCGACCCGAATGA 562 593 5269 Antisense
CAAGCAACGTCTCGGTATCATCCCG 636 185 5318 Antisense
CATCCCCCACAAAATCATACGTCAG 505 211 5428 Antisense
TCCTTGTCCATCGGCTATGGTTCAA 612 591 5460 Antisense
Sequence Source Affymetrix Proprietary Database