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[190423_at] serine/threonine kinase

Description C08F8.6 /REP_DB=WormBase Gene ID /WP=CE05253 /TR=Q17825 /GB=CAA97422.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 78      4grp_total > 31      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 10.6
(3)
A 		3.5
(-3)
A 		4.2
(-2.4)
A 		6.6
(-0.4)
A 		7
B 6.3
(3)
A 		5.7
(1.7)
A 		3.5
(-3)
A 		4.7
(-0.4)
A 		3
C 2.1
(-3)
A 		2.6
(-2.2)
A 		5.8
(3)
A 		3.6
(-0.6)
A 		4
avr 6.3
(3) 		3.9
(-3) 		4.5
(-1.5) 		5
(-0.3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 8
(-2.9)
A 		29.4
(-2.1)
A 		6.4
(-3)
A 		6.9
(-3)
A 		72.7
(-0.3)
A 		155.2
(3)
P 		149
B 6.1
(-2.9)
A 		3.5
(-3)
A 		3.7
(-3)
A 		4.7
(-2.9)
A 		27.4
(-1.8)
A 		126.1
(3)
M 		123
C 4.3
(-2.9)
A 		2.7
(-3)
A 		2.4
(-3)
A 		33.5
(-1.5)
A 		50.3
(-0.7)
A 		130.1
(3)
P 		128
avr 6.1
(-2.9) 		11.9
(-2.7) 		4.2
(-3) 		15
(-2.5) 		50.1
(-0.9) 		137.1
(3) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 3.9
(3)
A 		0.8
(-3)
A 		1.1
(-2.4)
A 		1
(-2.6)
A 		3
B 2
(3)
A 		1.4
(-0)
A 		0.8
(-3)
A 		1
(-2)
A 		1
C 0.7
(-2.6)
A 		0.8
(-2.1)
A 		2
(3)
A 		0.6
(-3)
A 		1
avr 2.2
(3) 		1
(-2.5) 		1.3
(-1.2) 		0.9
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 1.5
(-3)
A 		10.5
(-1.5)
A 		1.4
(-3)
A 		1.8
(-2.9)
A 		21.9
(0.4)
A 		37.8
(3)
P 		36
B 2.1
(-2.8)
A 		1.2
(-3)
A 		0.9
(-3)
A 		1.2
(-3)
A 		11.1
(-1.7)
A 		47
(3)
M 		46
C 1.6
(-2.9)
A 		0.8
(-3)
A 		0.6
(-3)
A 		6.7
(-2.1)
A 		16.3
(-0.8)
A 		43.6
(3)
P 		43
avr 1.7
(-2.9) 		4.2
(-2.5) 		1
(-3) 		3.2
(-2.7) 		16.4
(-0.8) 		42.8
(3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190423_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9888
Sequence Type Exemplar sequence
Representative Public ID C08F8.6 NCBI
Target Description C08F8.6 /REP_DB=WormBase Gene ID /WP=CE05253 /TR=Q17825 /GB=CAA97422.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069692(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069692 NCBI Caenorhabditis elegans C08F8.6 (C08F8.6) mRNA, complete cds. 11/11 None
SNAP00000012977 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11162758:11164260:-1 11/11 None
GENEFINDER00000012991 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11159663:11164260:-1 11/11 None
C08F8.6 ENSEMBL cdna:known chromosome:CEL140:IV:11162758:11164260:-1 gene:C08F8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11162763-11164266(-) 95.8 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Ensembl C08F8.6
Entrez Gene 182416 Entrez gene
SwissProt Q17825 EMBL-EBI
WormBase CE05253 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA97422 Hypothetical protein C08F8.6 [Caenorhabditis elegans] ref|NP_502093.1| C08F8.6 [Caenorhabditis elegans] 0.0
blast CAA97426 Hypothetical protein C08F8.5 [Caenorhabditis elegans] ref|NP_502092.1| C08F8.5 [Caenorhabditis elegans] 1.0E-180
blast CAE68129 Hypothetical protein CBG13774 [Caenorhabditis briggsae] 1.0E-175
blast CAE68129 Hypothetical protein CBG13774 [Caenorhabditis briggsae] 1.0E-174
blast CAA93431 Hypothetical protein ZK596.2 [Caenorhabditis elegans] ref|NP_502028.1| ZK596.2 [Caenorhabditis elegans] 1.0E-101
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.1E-19
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.3E-4
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.1E-4
Sequence
Target Sequence 
>C. ELEGANS:190423_AT
ctatcaagaagccccgtaacgtcgccgggttccgtgggactgtcaagtatgcgcctgtca
gttgtcatgctggacgtgaactctgtcgtcaggatgattgcgaaacgtggctttacatgg
tggttgagctcactaaagggtccctgccatggcgtaatatgacagaaatccaggatgttg
gaaatgagaagcgtgctattcgccgtgatcccatcgtcaagaagaagatgttcggaggct
gtccacgtgagtaccttgacattctcgaagcgatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATCAAGAAGCCCCGTAACGTCGC 192 225 530 Antisense
CGTGGGACTGTCAAGTATGCGCCTG 54 245 562 Antisense
TATGCGCCTGTCAGTTGTCATGCTG 588 655 577 Antisense
GTCATGCTGGACGTGAACTCTGTCG 291 467 593 Antisense
GAACTCTGTCGTCAGGATGATTGCG 435 349 607 Antisense
GCGAAACGTGGCTTTACATGGTGGT 148 289 629 Antisense
TGGTTGAGCTCACTAAAGGGTCCCT 551 549 650 Antisense
GGTCCCTGCCATGGCGTAATATGAC 524 501 668 Antisense
GAGAAGCGTGCTATTCGCCGTGATC 135 393 715 Antisense
GCTGTCCACGTGAGTACCTTGACAT 480 291 767 Antisense
GTACCTTGACATTCTCGAAGCGATC 71 459 780 Antisense
Sequence Source Affymetrix Proprietary Database