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[190422_at] WNT protein

Description W08D2.1 /REP_DB=WormBase Gene ID /WP=CE25152 /GEN=egl-20 /TR=Q9TVJ1 /GB=CAB61041.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=WNT protein
Cluster 6grp_total > 34      4grp_total > 30      6grp_cgc > 34      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 123.1
(3)
P 		67.6
(-1.2)
A 		44.7
(-3)
A 		109.7
(2)
M 		78
B 113.1
(3)
P 		112.2
(2.9)
A 		61.5
(-1.5)
A 		44.5
(-3)
P 		69
C 126.6
(0.9)
P 		63.5
(-3)
A 		66.3
(-2.8)
P 		159.4
(3)
P 		96
avr 120.9
(3) 		81.1
(-0.8) 		57.5
(-3) 		104.5
(1.4) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 91.3
(0.9)
A 		122.7
(3)
P 		109.6
(2.1)
P 		42
(-2.4)
A 		44.4
(-2.2)
A 		32.7
(-3)
A 		90
B 102
(3)
P 		35.6
(-3)
M 		95
(2.4)
P 		58.1
(-1)
A 		39.9
(-2.6)
P 		62.2
(-0.6)
M 		66
C 136.1
(3)
P 		124
(2.4)
P 		63.9
(-0.8)
P 		20.9
(-3)
A 		45.1
(-1.7)
A 		61.3
(-0.9)
P 		115
avr 109.8
(3) 		94.1
(1.6) 		89.5
(1.2) 		40.3
(-3) 		43.1
(-2.8) 		52.1
(-2) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 44.8
(3)
P 		14.7
(-2.5)
A 		11.7
(-3)
A 		16.1
(-2.2)
M 		33
B 36.1
(3)
P 		27.7
(1.1)
A 		14.3
(-1.9)
A 		9.5
(-3)
P 		27
C 41.9
(3)
P 		18.3
(-3)
A 		23.1
(-1.8)
P 		26.9
(-0.8)
P 		24
avr 40.9
(3) 		20.2
(-2.1) 		16.4
(-3) 		17.5
(-2.7) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 16.8
(-1.5)
A 		43.7
(3)
P 		23.9
(-0.3)
P 		10.6
(-2.6)
A 		13.4
(-2.1)
A 		8
(-3)
A 		36
B 35.5
(3)
P 		11.9
(-3)
M 		23.1
(-0.2)
P 		15.4
(-2.1)
A 		16.2
(-1.9)
P 		23.2
(-0.1)
M 		24
C 50.5
(3)
P 		37.8
(1.4)
P 		16.3
(-1.4)
P 		4.2
(-3)
A 		14.6
(-1.7)
A 		20.5
(-0.9)
P 		46
avr 34.3
(3) 		31.1
(2.2) 		21.1
(-0.3) 		10.1
(-3) 		14.7
(-1.9) 		17.2
(-1.2) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190422_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8593
Sequence Type Exemplar sequence
Representative Public ID W08D2.1 NCBI
Target Description W08D2.1 /REP_DB=WormBase Gene ID /WP=CE25152 /GEN=egl-20 /TR=Q9TVJ1 /GB=CAB61041.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=WNT protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069353(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069353 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-20) (egl-20) mRNA, complete cds. 11/11 None
SNAP00000011771 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9813157:9815495:-1 11/11 None
GENEFINDER00000011783 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9812951:9815495:-1 10/11 None
W08D2.1 ENSEMBL cdna:known chromosome:CEL140:IV:9812418:9815500:-1 gene:W08D2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9813162-9815501(-) 100.0 100.0
Public Domain and Genome References
Gene Title WNT protein
Gene Symbol egl-20
Ensembl W08D2.1
Entrez Gene 177821 Entrez gene
SwissProt Q9TVJ1 EMBL-EBI
WormBase CE25152 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGAAFFX.5670.1.S1_AT similar to WNT16 protein gga
MOE430A:1422941_AT wingless-related MMTV integration site 16 mm
MOUSE430A_2:1422941_AT wingless-related MMTV integration site 16 mm
MOUSE430_2:1422941_AT wingless-related MMTV integration site 16 mm
RAE230B:1385275_AT wingless-related MMTV integration site 16 rn
RAT230_2:1385275_AT wingless-related MMTV integration site 16 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7223 frizzled-2 signaling pathway inferred from electronic annotation QuickGO AmiGO
7275 development inferred from electronic annotation QuickGO AmiGO
16055 Wnt receptor signaling pathway inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
5623 cell inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5102 receptor binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61041 Hypothetical protein W08D2.1 [Caenorhabditis elegans] ref|NP_501754.1| EGg Laying defective family member (egl-20) [Caenorhabditis elegans] gb|AAD03603.1| Wnt homolog [Caenorhabditis elegans] 0.0
blast T26284 hypothetical protein W08D2.1 - Caenorhabditis elegans 0.0
blast CAE59984 Hypothetical protein CBG03476 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005817 EMBL-EBI Wnt superfamily 6.9E-125
Pfam IPR005817 EMBL-EBI Wnt superfamily 1.0E-122
Pfam IPR005817 EMBL-EBI Wnt superfamily 3.4E-103
Sequence
Target Sequence 
>C. ELEGANS:190422_AT
gatcagttctcctggggtggatgctctgataatgttccacatggtattcggtatgcaaaa
aagtttttggatgactgggaaactgcacagtttgacaaaacaaagaatgttgctcatctt
gttcgacggcataacaattttgttggaagagaggcaatcgcccaaaatattcgacgtcaa
tgtcgttgtcatggagtatctggttcatgtgaattcaaaacttgttggctccaaatgcag
aagttctcccaagtgtccgaccttcttaaaaagcgatatgatcatttcgcagttcaggta
acaagaaaagccactaaaagattacgaagaaaagaacgaacggaacgaaaaattccgttg
agaggaaatgaaatggcatacgtacatcgatcaccgtcttattgtgagaaaaatctcaca
gcaggaatactgggaacgtctggtcgtgaatgtattcataactcgtacagctcagaaagc
tgtgatttgctttgttgtggacgaggatataacacaagattagagatccgccaaactcaa
tgcgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAGTTCTCCTGGGGTGGATGCT 331 421 574 Antisense
TGTTCCACATGGTATTCGGTATGCA 221 561 606 Antisense
GTTGCTCATCTTGTTCGACGGCATA 275 439 682 Antisense
GCAATCGCCCAAAATATTCGACGTC 27 323 727 Antisense
GTCGTTGTCATGGAGTATCTGGTTC 680 473 755 Antisense
AATGCAGAAGTTCTCCCAAGTGTCC 619 165 807 Antisense
GTGTCCGACCTTCTTAAAAAGCGAT 339 439 826 Antisense
TACGTACATCGATCACCGTCTTATT 567 647 952 Antisense
AGGAATACTGGGAACGTCTGGTCGT 185 1 996 Antisense
GCTGTGATTTGCTTTGTTGTGGACG 455 295 1052 Antisense
GAGATCCGCCAAACTCAATGCGAGT 312 395 1096 Antisense
Sequence Source Affymetrix Proprietary Database