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[190415_s_at]

Description B0478.1 /REP_DB=WormBase Gene ID /WP=CE27574 /GEN=jnk-1 /TR=Q17507 /GB=AAA98724.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=MAP kinase
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 51      6grp_total > 24      4grp_total > 31      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 634
1 2 3 4 range
A 1008.8
(3)
P 		520.1
(-1.6)
P 		375.2
(-3)
P 		669.2
(-0.2)
P 		634
B 874.6
(3)
P 		440.8
(-2.3)
P 		380.8
(-3)
P 		826.9
(2.4)
P 		494
C 966.1
(3)
P 		455.2
(-3)
P 		454.4
(-3)
P 		697.7
(-0.1)
P 		512
avr 949.8
(3) 		472
(-2.2) 		403.5
(-3) 		731.3
(0.6) 		546

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 593
1 2 3 4 5 6 range
A 851.3
(3)
P 		674.7
(1.1)
P 		585.9
(0.2)
P 		448.5
(-1.3)
P 		308.8
(-2.8)
P 		285.4
(-3)
P 		566
B 878.4
(3)
P 		670.9
(0.8)
P 		710.2
(1.2)
P 		442.3
(-1.7)
P 		390.3
(-2.3)
P 		325
(-3)
P 		553
C 662.1
(1.7)
P 		744.3
(3)
P 		529
(-0.4)
P 		419.3
(-2.1)
P 		358.8
(-3)
P 		385.2
(-2.6)
P 		386
avr 797.3
(3) 		696.6
(1.7) 		608.4
(0.6) 		436.7
(-1.6) 		352.6
(-2.7) 		331.9
(-3) 		465
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 367.3
(3)
P 		112.9
(-2.7)
P 		98.1
(-3)
P 		98
(-3)
P 		269
B 278.9
(3)
P 		108.9
(-2.3)
P 		88.2
(-3)
P 		176.2
(-0.2)
P 		191
C 319.8
(3)
P 		130.9
(-2.6)
P 		158.4
(-1.8)
P 		117.6
(-3)
P 		202
avr 322
(3) 		117.6
(-2.9) 		114.9
(-3) 		130.6
(-2.5) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 156.9
(0.1)
P 		240
(3)
P 		127.6
(-1)
P 		113.7
(-1.4)
P 		93.2
(-2.2)
P 		69.5
(-3)
P 		171
B 305.6
(3)
P 		223.3
(0.4)
P 		172.4
(-1.2)
P 		117.1
(-3)
P 		158.6
(-1.7)
P 		121.2
(-2.9)
P 		189
C 245.4
(3)
P 		227.1
(2.3)
P 		134.6
(-1.1)
P 		84.3
(-3)
P 		116.1
(-1.8)
P 		128.9
(-1.3)
P 		161
avr 236
(3) 		230.1
(2.7) 		144.9
(-1.2) 		105
(-3) 		122.6
(-2.2) 		106.5
(-2.9) 		131
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190415_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9844
Sequence Type Exemplar sequence
Representative Public ID B0478.1 NCBI
Target Description B0478.1 /REP_DB=WormBase Gene ID /WP=CE27574 /GEN=jnk-1 /TR=Q17507 /GB=AAA98724.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=MAP kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026099(11), NM_171371(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026099 NCBI Caenorhabditis elegans Jun N-terminal Kinase family member (jnk-1) (jnk-1) mRNA, complete cds. 11/11 None
NM_171371 NCBI Caenorhabditis elegans Jun N-terminal Kinase family member (jnk-1) (jnk-1) mRNA, complete cds. 11/11 A 
SNAP00000002697 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6960165:6963576:1 11/11 None
GENEFINDER00000002699 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6957130:6963576:1 11/11 None
B0478.1a ENSEMBL cdna:known chromosome:CEL140:IV:6957625:6963700:1 gene:B0478.1 11/11 A 
B0478.1b.1 ENSEMBL cdna:known chromosome:CEL140:IV:6960190:6963576:1 gene:B0478.1 11/11 None
B0478.1b.2 ENSEMBL cdna:known chromosome:CEL140:IV:6960190:6963576:1 gene:B0478.1 11/11 None
B0478.1b.3 ENSEMBL cdna:known chromosome:CEL140:IV:6960193:6963576:1 gene:B0478.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6960502-6963582(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0478.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA98724 Jun n-terminal kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021270.1| Jun N-terminal Kinase family member (jnk-1) [Caenorhabditis elegans] sp|Q8WQG9|JNK1_CAEEL Stress-activated protein kinase jnk-1 dbj|BAA82640.1| JNK-1 [Caenorhabditis elegans] 0.0
blast CAE61773 Hypothetical protein CBG05733 [Caenorhabditis briggsae] 0.0
blast T30031 hypothetical protein B0478.1 - Caenorhabditis elegans 0.0
blast AAL50324 Jun n-terminal kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_741434.2| Jun N-terminal Kinase family member (jnk-1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-69
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-70
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-70
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 6.0E-71
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 8.0E-72
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 3.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-71
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-71
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-71
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-71
Sequence
Target Sequence 
>C. ELEGANS:190415_S_AT
gattggctcgaactgcgattgaggcattcatgatgactccttacgttgtgacaagatact
atcgggcaccggaagttattttaggaatgggttacaaggaaaatgttgatgtttggtcaa
ttgggtgtatatttggagagctgatacgtggacgtgtactattcccgggtggtgaccata
tcgatcagtggactaggattattgaacaattgggtactccggataggtcatttttagaac
ggttacaaccaactgtcagaaattatgtggaaaatcggccaagatatcaagcaacgccgt
ttgaagttctcttcagtgataacatgttcccaatgacagctgacagctcaagacttacag
gtgctcaagctcgtgacttactttctcgaatgttagtcatagatccggaaagaaggattt
ctgtggatgatgcactgagacatccatatgtcaatgtttggtttgatgaaattgaagtat
atgctccacctccacttccgtatgatcataatatggatgtggaacagaacgtggattcat
ggagagagcacattttccgagagctcacaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGGCTCGAACTGCGATTGAGGC 680 435 794 Antisense
CATGATGACTCCTTACGTTGTGACA 258 211 822 Antisense
GATACGTGGACGTGTACTATTCCCG 439 345 936 Antisense
ACTATTCCCGGGTGGTGACCATATC 650 91 951 Antisense
AATTGGGTACTCCGGATAGGTCATT 543 171 1001 Antisense
AGCAACGCCGTTTGAAGTTCTCTTC 572 75 1083 Antisense
TGTTCCCAATGACAGCTGACAGCTC 352 563 1118 Antisense
AAGACTTACAGGTGCTCAAGCTCGT 406 133 1143 Antisense
CAAGCTCGTGACTTACTTTCTCGAA 690 185 1159 Antisense
CCTCCACTTCCGTATGATCATAATA 247 267 1282 Antisense
AGCACATTTTCCGAGAGCTCACAGA 63 77 1340 Antisense
Sequence Source Affymetrix Proprietary Database