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[190413_at] asparagine synthetase

Description F25G6.6 /REP_DB=WormBase Gene ID /WP=CE09642 /TR=O16924 /GB=AAC25799.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=asparagine synthetase
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 90      4grp_highest_8_8 > 01      4grp_high_8_8 > 54      6grp_highest_nonorm > 72      6grp_total > 90      4grp_total > 58      6grp_cgc > 96      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 487
1 2 3 4 range
A 257.1
(-1.2)
P 		517.9
(2.4)
P 		560.8
(3)
P 		125.5
(-3)
P 		435
B 187.6
(-1.6)
P 		548
(3)
P 		538.2
(2.9)
P 		81.7
(-3)
P 		466
C 202.7
(-1.9)
P 		568.5
(3)
P 		466.6
(1.6)
P 		122.8
(-3)
P 		446
avr 215.8
(-1.5) 		544.8
(3) 		521.9
(2.7) 		110
(-3) 		435

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 323.6
(-3)
P 		396.3
(0.1)
P 		323.3
(-3)
P 		466.6
(3)
P 		424.4
(1.2)
P 		449.8
(2.3)
P 		143
B 356.3
(-0.4)
P 		468.9
(3)
P 		267.8
(-3)
P 		453
(2.5)
P 		409.2
(1.2)
P 		365.8
(-0.1)
P 		201
C 326.9
(-3)
P 		497.2
(1.4)
P 		381.5
(-1.6)
P 		558.4
(3)
P 		397.5
(-1.2)
P 		384.6
(-1.5)
P 		232
avr 335.6
(-2.6) 		454.1
(1.6) 		324.2
(-3) 		492.7
(3) 		410.4
(0.1) 		400.1
(-0.3) 		169
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 146
1 2 3 4 range
A 93.6
(0.5)
P 		112.4
(1.4)
P 		146.6
(3)
P 		18.4
(-3)
P 		128
B 59.8
(-0.8)
P 		135.4
(3)
P 		124.7
(2.5)
P 		17.4
(-3)
P 		118
C 67.1
(-1)
P 		163.4
(3)
P 		162.6
(3)
P 		20.7
(-3)
P 		143
avr 73.5
(-0.4) 		137.1
(2.6) 		144.6
(3) 		18.8
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 59.7
(-3)
P 		141
(3)
P 		70.4
(-2.2)
P 		118.3
(1.3)
P 		128.1
(2)
P 		109.5
(0.7)
P 		81
B 124
(0.5)
P 		156.1
(2.4)
P 		65
(-3)
P 		120
(0.3)
P 		166.3
(3)
P 		136.4
(1.2)
P 		101
C 121.2
(-0.4)
P 		151.7
(3)
P 		97.1
(-3)
P 		112.3
(-1.3)
P 		128.6
(0.5)
P 		128.7
(0.5)
P 		55
avr 101.6
(-1) 		149.6
(3) 		77.5
(-3) 		116.9
(0.3) 		141
(2.3) 		124.9
(0.9) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190413_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11615
Sequence Type Exemplar sequence
Representative Public ID F25G6.6 NCBI
Target Description F25G6.6 /REP_DB=WormBase Gene ID /WP=CE09642 /TR=O16924 /GB=AAC25799.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=asparagine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072803 NCBI Caenorhabditis elegans asparagiNyl tRNA Synthetase family member (nrs-2) (nrs-2) mRNA, complete cds. 11/11 None
SNAP00000003976 ENSEMBL cdna:SNAP chromosome:CEL140:V:8548876:8551106:1 11/11 None
GENEFINDER00000003987 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8548876:8551106:1 11/11 None
F25G6.6 ENSEMBL cdna:known chromosome:CEL140:V:8548876:8551460:1 gene:F25G6.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8548876-8551107(+) 100.0 100.0
Public Domain and Genome References
Gene Title asparagine synthetase
Gene Symbol nrs-2
Ensembl F25G6.6
Entrez Gene 184935 Entrez gene
SwissProt O16924 EMBL-EBI
WormBase CE09642 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584041_AT similar to asparagine synthetase cfa
CANINE_2:CFA.12008.1.A1_AT similar to asparagine synthetase cfa
CANINE_2:CFA.12008.1.A1_S_AT similar to asparagine synthetase cfa
CANINE_2:CFA.19985.1.S1_S_AT similar to asparagine synthetase cfa
CHICKEN:GGAAFFX.6121.1.S1_S_AT asparagine synthetase gga
HG-U133_PLUS_2:205047_S_AT asparagine synthetase hs
HG-U133A:205047_S_AT asparagine synthetase hs
HG-FOCUS:205047_S_AT asparagine synthetase hs
HG-U133A_2:205047_S_AT asparagine synthetase hs
HG-U95AV2:36671_AT asparagine synthetase hs
HUGENEFL:M27396_S_AT asparagine synthetase hs
U133_X3P:G4502258_3P_A_AT asparagine synthetase hs
HG-U95E:70493_AT asparagine synthetase hs
HG-U133_PLUS_2:241082_AT Asparagine synthetase hs
HG-U133B:241082_AT Asparagine synthetase hs
HG-U95E:69935_AT Asparagine synthetase hs
U133_X3P:HS.202216.0.A1_3P_AT Asparagine synthetase hs
MU11KSUBA:U38940_S_AT asparagine synthetase mm
MG-U74AV2:95133_AT asparagine synthetase mm
MG-U74CV2:136281_AT asparagine synthetase mm
MG-U74AV2:161221_F_AT asparagine synthetase mm
MOE430A:1451095_AT asparagine synthetase mm
MOUSE430A_2:1451095_AT asparagine synthetase mm
MOUSE430_2:1451095_AT asparagine synthetase mm
MOE430A:1433966_X_AT asparagine synthetase mm
MOUSE430A_2:1433966_X_AT asparagine synthetase mm
MOUSE430_2:1433966_X_AT asparagine synthetase mm
MG-U74CV2:169130_I_AT Asparagine synthetase, mRNA (cDNA clone MGC:5995 IMAGE:3585775) mm
MG-U74CV2:171160_R_AT Asparagine synthetase, mRNA (cDNA clone MGC:5995 IMAGE:3585775) mm
RG-U34A:U07201_AT asparagine synthetase rn
RAE230A:1387925_AT asparagine synthetase rn
RAT230_2:1387925_AT asparagine synthetase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6529 asparagine biosynthesis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4066 asparagine synthase (glutamine-hydrolyzing) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC25799 Asparaginyl trna synthetase protein 2 [Caenorhabditis elegans] ref|NP_505204.1| asparagiNyl tRNA Synthetase family member (nrs-2) [Caenorhabditis elegans] 0.0
blast CAE58368 Hypothetical protein CBG01494 [Caenorhabditis briggsae] 0.0
blast CAB96680 asparagine synthetase ASN3 [Arabidopsis thaliana] gb|AAO50547.1| putative asparagine synthetase ASN3 [Arabidopsis thaliana] gb|AAO41976.1| putative asparagine synthetase ASN3 [Arabidopsis thaliana] ref|NP_196586.1| asparagine synthetase 3 (ASN3) [Arabidopsis thaliana] 8.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR001962 EMBL-EBI Asparagine synthase 1.0E-126
Pfam IPR001962 EMBL-EBI Asparagine synthase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:190413_AT
aaagagctccaaatgcactccatcttcataaagaaatcctccgtagaatgcatcatctcc
accaatatgacgtccttcgttgtgatcgatccacttcttgtcacggactcgaaatccgag
ttccattccttgacaaacgattcatcgatcttgtgagccgtcttccaccatcatataagc
ttatgcctatgaaattagaaaaacatgttttgagaagtgcttttgaaggatggcttccgg
atgaagttctgtggagaagcaaagaaggtttcgccgaggctctcggtaaaactgatttgg
gagatattctcgctgaacattgtgataagctgatttcaaagagtttgtatgagcgtcgtg
ctgaaagattcccggacaggacaccagaaacaacagaagaatattggtatcgccaaattt
ttgaggacaccttcgtctatgataagatgggacatttggtgcacactaaagtttatagaa
cggctgcctggcaccgacctgacgagaaggagaatatgcacctagaaaccgagacattgg
atgtaaaaaccgatgatcttctccgacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGAGCTCCAAATGCACTCCATCT 476 123 1064 Antisense
GAAATCCTCCGTAGAATGCATCATC 81 363 1096 Antisense
GCATCATCTCCACCAATATGACGTC 253 309 1113 Antisense
TGACGTCCTTCGTTGTGATCGATCC 284 571 1131 Antisense
TCCACTTCTTGTCACGGACTCGAAA 97 587 1153 Antisense
CCGAGTTCCATTCCTTGACAAACGA 673 261 1179 Antisense
AGCGTCGTGCTGAAAGATTCCCGGA 535 81 1415 Antisense
GATTCCCGGACAGGACACCAGAAAC 320 433 1430 Antisense
AGATGGGACATTTGGTGCACACTAA 213 67 1508 Antisense
TTTATAGAACGGCTGCCTGGCACCG 314 671 1535 Antisense
AAAAACCGATGATCTTCTCCGACTA 644 129 1608 Antisense
Sequence Source Affymetrix Proprietary Database