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[190412_s_at] Enoyl-CoA hydratase

Description T05G5.6 /REP_DB=WormBase Gene ID /WP=CE00318 /TR=SW:P34559 /GB=CAA81594.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Enoyl-CoA hydratase
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 26      6grp_total > 52      4grp_total > 56      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6150
1 2 3 4 range
A 2925
(-1.3)
P 		5206.5
(1.6)
P 		6331
(3)
P 		1563.8
(-3)
P 		4767
B 3181.7
(-1.1)
P 		4446.3
(0.2)
P 		7365.8
(3)
P 		1215.9
(-3)
P 		6150
C 3217.6
(-0.8)
P 		4518.2
(0.7)
P 		6514.9
(3)
P 		1321.1
(-3)
P 		5194
avr 3108.1
(-1.1) 		4723.7
(0.8) 		6737.2
(3) 		1366.9
(-3) 		5370

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4547
1 2 3 4 5 6 range
A 4526.7
(-1)
P 		5211.6
(-0.1)
P 		7577.7
(3)
P 		5605.9
(0.4)
P 		3952.1
(-1.8)
P 		3030.8
(-3)
P 		4547
B 3920.7
(-2.6)
P 		5352.6
(-0.3)
P 		7345.5
(3)
P 		5651.1
(0.2)
P 		4407
(-1.8)
P 		3669.1
(-3)
P 		3676
C 3543.5
(-3)
P 		4974.2
(-0.6)
P 		7152.4
(3)
P 		6334.2
(1.6)
P 		3697.7
(-2.7)
P 		3940.4
(-2.3)
P 		3609
avr 3997
(-2.3) 		5179.5
(-0.4) 		7358.5
(3) 		5863.7
(0.6) 		4018.9
(-2.3) 		3546.8
(-3) 		3812
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2048
1 2 3 4 range
A 1065
(0.5)
P 		1130.2
(0.8)
P 		1655.1
(3)
P 		228.9
(-3)
P 		1426
B 1014.6
(0.1)
P 		1098.1
(0.5)
P 		1706.8
(3)
P 		259.1
(-3)
P 		1448
C 1065.1
(-0.5)
P 		1299
(0.2)
P 		2270.7
(3)
P 		222.8
(-3)
P 		2048
avr 1048.2
(-0) 		1175.8
(0.4) 		1877.5
(3) 		236.9
(-3) 		1641

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1116
1 2 3 4 5 6 range
A 834.5
(-2.5)
P 		1853.5
(3)
P 		1650.3
(1.9)
P 		1421.8
(0.7)
P 		1192.6
(-0.6)
P 		737.9
(-3)
P 		1116
B 1364.2
(-3)
P 		1781.7
(2.9)
P 		1783.7
(2.9)
P 		1496.8
(-1.1)
P 		1790.5
(3)
P 		1368.2
(-2.9)
P 		426
C 1313.4
(-1.9)
P 		1517.8
(0.1)
P 		1820.4
(3)
P 		1273.8
(-2.3)
P 		1196.5
(-3)
P 		1319.1
(-1.8)
P 		624
avr 1170.7
(-2.7) 		1717.7
(2.7) 		1751.5
(3) 		1397.5
(-0.5) 		1393.2
(-0.5) 		1141.7
(-3) 		610
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190412_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8081
Sequence Type Exemplar sequence
Representative Public ID T05G5.6 NCBI
Target Description T05G5.6 /REP_DB=WormBase Gene ID /WP=CE00318 /TR=SW:P34559 /GB=CAA81594.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Enoyl-CoA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066755 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-6) (ech-6) mRNA, complete cds. 11/11 None
GENEFINDER00000019887 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9750994:9752770:-1 10/11 None
SNAP00000019880 ENSEMBL cdna:SNAP chromosome:CEL140:III:9751588:9752770:-1 11/11 None
T05G5.6.2 ENSEMBL cdna:known chromosome:CEL140:III:9751450:9752780:-1 gene:T05G5.6 11/11 None
T05G5.6.1 ENSEMBL cdna:known chromosome:CEL140:III:9751450:9752778:-1 gene:T05G5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9751583-9752766(-) 100.0 100.0
Public Domain and Genome References
Gene Title Enoyl-CoA hydratase
Gene Symbol ech-6
Ensembl T05G5.6
Entrez Gene 176376 Entrez gene
SwissProt P34559 EMBL-EBI
WormBase CE00318 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1640466_S_AT dm
DROSGENOME1:142709_S_AT dm
HG-U133_PLUS_2:201135_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
HG-FOCUS:201135_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
HG-U133A:201135_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
HG-U133A_2:201135_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
HUGENEFL:D13900_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
U133_X3P:G12707569_3P_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
HG-U95AV2:37016_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial hs
MOUSE430_2:1452341_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial mm
MOE430A:1452341_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial mm
MOUSE430A_2:1452341_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial mm
MU11KSUBA:AA270965_S_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial mm
MG-U74AV2:95426_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial mm
RG-U34A:X15958_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial rn
RAE230A:1367829_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial rn
RAT230_2:1367829_AT enoyl Coenzyme A hydratase, short chain, 1, mitochondrial rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA81594 Hypothetical protein T05G5.6 [Caenorhabditis elegans] ref|NP_499156.1| Enoyl-CoA Hydratase family member (ech-6) [Caenorhabditis elegans] sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial 1.0E-159
blast CAE65137 Hypothetical protein CBG10003 [Caenorhabditis briggsae] 1.0E-151
blast CAA81594 Hypothetical protein T05G5.6 [Caenorhabditis elegans] ref|NP_499156.1| Enoyl-CoA Hydratase family member (ech-6) [Caenorhabditis elegans] sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial 1.0E-149
blast CAE65114 Hypothetical protein CBG09979 [Caenorhabditis briggsae] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 3.0E-92
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019887 1 323-345
Sequence
Target Sequence 
>C. ELEGANS:190412_S_AT
gagcttttgccgctggagctgatatcaaggagatgaccaataacgagttcgccaccacat
tttctggaagcttcctctccaactggaccgctgtcagtgatgtgaaaaagccagttattg
ctgcagtgaacggatttgcattgggaggtggaaacgagttggcaatgatgtgtgatatta
tctatgccggagagaaggctcgttttggacaacctgaaattaacattggaactattccag
gagcaggaggtactcagcgttgggctcgcgccgccggaaaatcatttgccatggaggtgt
gccttactggaaaccacgtgactgctcaagaagctaaggagcacggtattgtgagcaaga
ttttcccagctgatcaggttgtcggggaagctgtgaaacttggagagaaaatcgctgatc
aatctccactgattgttcaaatggcaaaggaggccgttaacaaagcttatgagcttactc
ttcaagagggacttcacttcgaaagacgacttttccatgccacttttgccaccaaggata
gaaaggaaggaatgaccgcttttgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTTTTGCCGCTGGAGCTGATAT 493 387 281 Antisense
GTTCGCCACCACATTTTCTGGAAGC 43 443 327 Antisense
CTCCAACTGGACCGCTGTCAGTGAT 241 237 357 Antisense
AGCAGGAGGTACTCAGCGTTGGGCT 39 75 522 Antisense
TGCCATGGAGGTGTGCCTTACTGGA 667 585 567 Antisense
TACTGGAAACCACGTGACTGCTCAA 620 647 585 Antisense
GTGAGCAAGATTTTCCCAGCTGATC 480 483 631 Antisense
GAAAATCGCTGATCAATCTCCACTG 463 353 687 Antisense
CGAAAGACGACTTTTCCATGCCACT 408 251 780 Antisense
TGCCACTTTTGCCACCAAGGATAGA 388 587 798 Antisense
AGGAAGGAATGACCGCTTTTGCCGA 702 53 824 Antisense
Sequence Source Affymetrix Proprietary Database