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[190407_at]

Description ZK250.1 /REP_DB=WormBase Gene ID /WP=CE15267 /TR=O17305 /GB=AAB71070.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse transcriptase
Cluster 6grp_total > 40      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 20.5
(-3)
A 		31.6
(-1.7)
A 		38.4
(-0.8)
A 		70.3
(3)
P 		50
B 16
(-1.4)
A 		53.5
(3)
P 		2.2
(-3)
A 		53.8
(3)
A 		52
C 44.3
(-2.2)
P 		33.9
(-3)
A 		36.3
(-2.8)
A 		113.9
(3)
P 		80
avr 26.9
(-2.9) 		39.7
(-1.4) 		25.6
(-3) 		79.3
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 7.6
(-3)
A 		17.8
(-0.2)
A 		20.2
(0.5)
A 		29.2
(3)
A 		20.8
(0.6)
A 		29.3
(3)
A 		22
B 8.2
(-3)
A 		32.6
(2.3)
A 		35.9
(3)
A 		27.7
(1.2)
A 		21.8
(-0.1)
A 		15.6
(-1.4)
A 		28
C 39.7
(2.6)
A 		35.7
(1.9)
P 		24.7
(0)
A 		42.1
(3)
A 		6.9
(-3)
A 		14.1
(-1.8)
A 		35
avr 18.5
(-2.3) 		28.7
(1.4) 		26.9
(0.8) 		33
(3) 		16.5
(-3) 		19.7
(-1.8) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 7.5
(-1.9)
A 		6.9
(-3)
A 		10
(2.5)
A 		10.3
(3)
P 		3
B 5.1
(-0.8)
A 		13.2
(3)
P 		0.5
(-3)
A 		11.5
(2.2)
A 		13
C 14.7
(0.1)
P 		9.8
(-3)
A 		12.7
(-1.1)
A 		19.2
(3)
P 		9
avr 9.1
(-1.6) 		10
(-0.7) 		7.7
(-3) 		13.7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1.4
(-3)
A 		6.3
(1.9)
A 		4.4
(0)
A 		7.4
(3)
A 		6.3
(1.9)
A 		7.1
(2.7)
A 		6
B 2.9
(-3)
A 		10.9
(3)
A 		8.7
(1.4)
A 		7.3
(0.3)
A 		8.9
(1.5)
A 		5.8
(-0.8)
A 		8
C 14.7
(3)
A 		10.9
(1.2)
P 		6.3
(-1)
A 		8.5
(0)
A 		2.2
(-3)
A 		4.7
(-1.8)
A 		13
avr 6.3
(-2.2) 		9.4
(3) 		6.5
(-1.8) 		7.7
(0.2) 		5.8
(-3) 		5.9
(-2.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5510
Sequence Type Exemplar sequence
Representative Public ID ZK250.1 NCBI
Target Description ZK250.1 /REP_DB=WormBase Gene ID /WP=CE15267 /TR=O17305 /GB=AAB71070.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse transcriptase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061739(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061739 NCBI Caenorhabditis elegans ZK250.1 (ZK250.1) mRNA, complete cds. 11/11 None
ZK250.1 ENSEMBL cdna:known chromosome:CEL140:II:1959109:1961570:1 gene:ZK250.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182462 3/11 Cross Hyb Matching Probes None
F58D12.2 5/11 Cross Hyb Matching Probes None
Y108F1.2 3/11 Cross Hyb Matching Probes None
F23A7.2 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1959107-1960810(+) 47.0 69.4
chrV:14043584-14045278(+) 46.46 68.59
chrV:14053936-14055625(-) 46.46 68.37
Public Domain and Genome References
Ensembl ZK250.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494140 ZK250.1 [Caenorhabditis elegans] 0.0
blast T25005 hypothetical protein T19C9.7 - Caenorhabditis elegans 0.0
blast NP_508294 H02F09.4 [Caenorhabditis elegans] 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 1.6E-7
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.3E-50
Sequence
Target Sequence 
>C. ELEGANS:190407_AT
gataacatttgcttaactcttctggtgtcctacaaggcactgtcactggacccttttttt
ttggtctatataaatgaccttcttgatctattcccccctgatgttcacgttattgcattt
gccgacgatctcaaacttttcagcaataactgtcaaagcttgcgttcatctataaaaata
attgaaaactggtgtaaaatttggcagctcaagctagccgaaaataaaacaaaggtactt
catgttggtaagaaaaaccctaaatataaatattatgtaaacggacaaaagattgaatgt
tgttctaaagcttgtgatcttggcatatgggtcgatgatcaattaacttatgaaaaacac
attctagtcaaagtaaatgcagcaatgtttaaatgccgccaaatactcaaaaacttccgc
tctcttaatatgatgttctacttcaaattatttaatacttaccagccgttcgcacgcgcc
aaggcgcgtgctccgtctggctttgtagtttctaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAACATTTGCTTAACTCTTCTGG 512 423 1627 Antisense
ACTCTTCTGGTGTCCTACAAGGCAC 107 101 1642 Antisense
TAAATGACCTTCTTGATCTATTCCC 428 641 1697 Antisense
TGTTCACGTTATTGCATTTGCCGAC 506 561 1728 Antisense
TGCCGACGATCTCAAACTTTTCAGC 590 583 1746 Antisense
AACTGTCAAAGCTTGCGTTCATCTA 157 143 1774 Antisense
ATTTGGCAGCTCAAGCTAGCCGAAA 606 17 1825 Antisense
GCTTGTGATCTTGGCATATGGGTCG 383 307 1936 Antisense
CAAAAACTTCCGCTCTCTTAATATG 93 189 2034 Antisense
TATTTAATACTTACCAGCCGTTCGC 601 661 2075 Antisense
TCCGTCTGGCTTTGTAGTTTCTAGA 421 595 2118 Antisense
Sequence Source Affymetrix Proprietary Database