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[190404_s_at] folate transporter

Description F37B4.7 /REP_DB=WormBase Gene ID /WP=CE17796 /TR=SW:O45166 /GB=AAK18943.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      6grp_highest_nonorm > 81      6grp_total > 51      4grp_total > 56      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1067
1 2 3 4 range
A 174.5
(-2)
P 		323.6
(-1.1)
P 		1017.3
(3)
P 		7.7
(-3)
A 		1010
B 212.1
(-2)
P 		240.3
(-1.9)
P 		1074.6
(3)
P 		43.6
(-3)
A 		1031
C 133.1
(-2.4)
P 		250.5
(-1.6)
P 		944.4
(3)
P 		45
(-3)
A 		899
avr 173.2
(-2.1) 		271.5
(-1.5) 		1012.1
(3) 		32.1
(-3) 		980

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 267.4
(-0.8)
P 		304.5
(0.1)
P 		432.5
(3)
P 		329.3
(0.6)
P 		169.8
(-3)
P 		247.2
(-1.2)
P 		263
B 195.8
(-3)
P 		233.9
(-1.8)
P 		348.7
(1.9)
P 		383.4
(3)
P 		208
(-2.6)
P 		195.4
(-3)
P 		188
C 229.6
(-2.4)
P 		284.1
(-0.2)
P 		364.5
(3)
P 		320.8
(1.3)
P 		214.5
(-3)
P 		257.7
(-1.3)
P 		150
avr 230.9
(-1.9) 		274.2
(-0.5) 		381.9
(3) 		344.5
(1.8) 		197.4
(-3) 		233.4
(-1.8) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 328
1 2 3 4 range
A 63.5
(-1.6)
P 		70.3
(-1.4)
P 		265.9
(3)
P 		1.1
(-3)
A 		265
B 67.6
(-1.5)
P 		59.4
(-1.7)
P 		249
(3)
P 		9.3
(-3)
A 		240
C 44.1
(-2.3)
P 		72
(-1.8)
P 		329.2
(3)
P 		7.6
(-3)
A 		322
avr 58.4
(-1.9) 		67.2
(-1.7) 		281.4
(3) 		6
(-3) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 49.3
(-3)
P 		108.3
(3)
P 		94.2
(1.6)
P 		83.5
(0.5)
P 		51.2
(-2.8)
P 		60.2
(-1.9)
P 		59
B 68.1
(-3)
P 		77.9
(-1.2)
P 		84.7
(-0)
P 		101.5
(3)
P 		84.5
(-0.1)
P 		72.9
(-2.1)
P 		33
C 85.1
(1.4)
P 		86.7
(1.7)
P 		92.8
(3)
P 		64.5
(-3)
P 		69.4
(-2)
P 		86.3
(1.6)
P 		28
avr 67.5
(-3) 		91
(3) 		90.6
(2.9) 		83.2
(1) 		68.4
(-2.8) 		73.1
(-1.6) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190404_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15765
Sequence Type Exemplar sequence
Representative Public ID F37B4.7 NCBI
Target Description F37B4.7 /REP_DB=WormBase Gene ID /WP=CE17796 /TR=SW:O45166 /GB=AAK18943.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071379(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071379 NCBI Caenorhabditis elegans F37B4.7 (F37B4.7) mRNA, complete cds. 11/11 None
SNAP00000026931 ENSEMBL cdna:SNAP chromosome:CEL140:V:2872542:2875305:-1 11/11 None
GENEFINDER00000026942 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2872542:2874746:-1 11/11 None
F37B4.7.1 ENSEMBL cdna:known chromosome:CEL140:V:2872292:2875427:-1 gene:F37B4.7 11/11 None
F37B4.7.2 ENSEMBL cdna:known chromosome:CEL140:V:2872365:2875459:-1 gene:F37B4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2872541-2875305(-) 100.0 100.0
Public Domain and Genome References
Gene Title folate transporter
Ensembl F37B4.7
Entrez Gene 178745 Entrez gene
SwissProt O45166 EMBL-EBI
WormBase CE17796 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1623916_AT dm
DROSGENOME1:149651_AT dm
CHICKEN:GGA.15937.1.S1_AT similar to thiamine transporter gga
CHICKEN:GGAAFFX.9688.2.S1_AT similar to thiamine transporter gga
CHICKEN:GGAAFFX.9688.3.S1_S_AT similar to thiamine transporter gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5542 folic acid binding inferred from electronic annotation QuickGO AmiGO
8518 reduced folate carrier activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK18943 Hypothetical protein F37B4.7 [Caenorhabditis elegans] ref|NP_503780.1| F37B4.7 [Caenorhabditis elegans] sp|O45166|FOLT3_CAEEL Folate-like transporter 0.0
blast CAE58079 Hypothetical protein CBG01160 [Caenorhabditis briggsae] 0.0
blast CAE70448 Hypothetical protein CBG17027 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503780.1 8 45-67,72-94,101-119,132-154,161-183,316-338,359-381,391-413
F37B4.7.1 8 45-67,72-94,101-119,132-154,161-183,316-338,359-381,391-413
F37B4.7.2 8 45-67,72-94,101-119,132-154,161-183,316-338,359-381,391-413
SNAP00000026931 8 45-67,72-94,101-119,132-154,161-183,316-338,359-381,391-413
GENEFINDER00000026942 8 69-91,101-123,130-148,161-183,190-212,345-367,388-410,420-442
Sequence
Target Sequence 
>C. ELEGANS:190404_S_AT
tgttctttgtcgggttgcggcataatttaatggcgatttatcgcaatccgctgattttga
agtggtctgtttggtcggctctttcgagttgcattttttatcaggtcacaaactacaccc
aaactttatggggtacacttccggagtctgcgaatcgttataatggtatcaccgaagccc
tcgtcccacttcttggcatcccagctgacctgatcacccgccagctcaacgtgaattgga
accgatggggagaccttctccttgctgttggctccatcggacaagccggcctactttttt
ggatgtcacaatctcatcatatcgtggtgttatacctgagttacatattctatagagtca
tataccagttgaccactacgattgctcagtctacactggccttttccctggactctagac
tatttggcctactcttcgggattaacacatttgttgctctccttcttcaatctattctga
ccgctgttgtcatagattggcagaagctggatattaggccacagtttgtggtctattcat
gttatcatcttgtcgttgcatttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTCTTTGTCGGGTTGCGGCATAA 590 559 662 Antisense
GCGATTTATCGCAATCCGCTGATTT 235 291 694 Antisense
TTGAAGTGGTCTGTTTGGTCGGCTC 41 691 718 Antisense
TACACTTCCGGAGTCTGCGAATCGT 380 641 795 Antisense
ATAATGGTATCACCGAAGCCCTCGT 322 25 821 Antisense
ACCCGCCAGCTCAACGTGAATTGGA 140 91 877 Antisense
TGCTGTTGGCTCCATCGGACAAGCC 363 583 924 Antisense
TTGACCACTACGATTGCTCAGTCTA 179 703 1030 Antisense
TGGCCTACTCTTCGGGATTAACACA 667 541 1086 Antisense
TCTCCTTCTTCAATCTATTCTGACC 671 609 1119 Antisense
GTTATCATCTTGTCGTTGCATTTGG 635 447 1202 Antisense
Sequence Source Affymetrix Proprietary Database