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[190395_at] mechanosensory protein

Description T28B8.5 /REP_DB=WormBase Gene ID /WP=CE25129 /TR=P91835 /GB=CAB03443.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mechanosensory protein
Cluster 6grp_total > 14      4grp_total > 46      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 115.4
(3)
A 		18.2
(-2.3)
A 		6.2
(-3)
A 		62.3
(0.1)
A 		109
B 117.1
(3)
A 		51.5
(-0.5)
A 		7.2
(-2.8)
A 		3.3
(-3)
A 		114
C 119.3
(3)
A 		75.4
(0.7)
A 		6.4
(-2.9)
A 		5.3
(-3)
A 		114
avr 117.3
(3) 		48.4
(-0.7) 		6.6
(-3) 		23.6
(-2.1) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 104.4
(3)
A 		44.1
(-0.6)
A 		38.8
(-0.9)
A 		64.9
(0.7)
A 		43.3
(-0.6)
A 		3.2
(-3)
A 		101
B 97.6
(3)
A 		81.3
(2)
A 		44.3
(-0.4)
A 		32.7
(-1.1)
A 		32.7
(-1.1)
A 		2.8
(-3)
A 		95
C 92.4
(1.5)
A 		121
(3)
A 		50
(-0.8)
A 		26.8
(-2)
A 		8.2
(-3)
A 		19.7
(-2.4)
A 		113
avr 98.1
(3) 		82.1
(1.9) 		44.4
(-0.6) 		41.5
(-0.8) 		28.1
(-1.7) 		8.6
(-3) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 42
(3)
A 		4
(-2.6)
A 		1.6
(-3)
A 		9.1
(-1.9)
A 		40
B 37.3
(3)
A 		12.7
(-1)
A 		1.7
(-2.8)
A 		0.7
(-3)
A 		37
C 39.5
(3)
A 		21.7
(0.2)
A 		2.2
(-2.8)
A 		0.9
(-3)
A 		39
avr 39.6
(3) 		12.8
(-1.3) 		1.8
(-3) 		3.6
(-2.7) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 19.2
(3)
A 		15.7
(1.9)
A 		8.4
(-0.5)
A 		16.5
(2.1)
A 		13.1
(1)
A 		0.8
(-3)
A 		18
B 34
(3)
A 		27
(1.7)
A 		10.8
(-1.2)
A 		8.7
(-1.6)
A 		13.3
(-0.8)
A 		1
(-3)
A 		33
C 34.2
(2.5)
A 		36.9
(3)
A 		12.7
(-1.2)
A 		5.4
(-2.5)
A 		2.7
(-3)
A 		6.6
(-2.3)
A 		34
avr 29.1
(3) 		26.5
(2.4) 		10.6
(-1.2) 		10.2
(-1.3) 		9.7
(-1.4) 		2.8
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190395_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.793
Sequence Type Exemplar sequence
Representative Public ID T28B8.5 NCBI
Target Description T28B8.5 /REP_DB=WormBase Gene ID /WP=CE25129 /TR=P91835 /GB=CAB03443.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mechanosensory protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059829 NCBI Caenorhabditis elegans T28B8.5 (T28B8.5) mRNA, complete cds. 11/11 None
SNAP00000004693 ENSEMBL cdna:SNAP chromosome:CEL140:I:8147631:8150663:-1 11/11 None
GENEFINDER00000004704 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8147631:8150663:-1 11/11 None
T28B8.5 ENSEMBL cdna:known chromosome:CEL140:I:8147594:8150663:-1 gene:T28B8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8147561-8150594(-) 100.0 100.0
Public Domain and Genome References
Gene Title mechanosensory protein
Ensembl T28B8.5
Entrez Gene 189033 Entrez gene
SwissProt P91835 EMBL-EBI
WormBase CE32505 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27077.1.S1_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) cfa
CANINE_2:CFAAFFX.27080.1.S1_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) cfa
HG-U95AV2:39682_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
HG-U133_PLUS_2:205464_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
HG-U133A:205464_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
HG-FOCUS:205464_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
HG-U133A_2:205464_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
HUGENEFL:X87159_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
U133_X3P:G4506816_3P_AT sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) hs
MOE430A:1418475_AT sodium channel, nonvoltage-gated 1 beta mm
MOUSE430A_2:1418475_AT sodium channel, nonvoltage-gated 1 beta mm
MOUSE430_2:1418475_AT sodium channel, nonvoltage-gated 1 beta mm
MG-U74BV2:110692_AT sodium channel, nonvoltage-gated 1 beta mm
RN-U34:X77932_AT sodium channel, nonvoltage-gated 1 beta rn
RG-U34A:X77932_AT sodium channel, nonvoltage-gated 1 beta rn
RAE230A:1370482_AT sodium channel, nonvoltage-gated 1 beta rn
RAT230_2:1370482_AT sodium channel, nonvoltage-gated 1 beta rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03443 Hypothetical protein T28B8.5 [Caenorhabditis elegans] ref|NP_492230.2| T28B8.5 [Caenorhabditis elegans] 0.0
blast CAE57373 Hypothetical protein CBG00319 [Caenorhabditis briggsae] 0.0
blast CAA96693 Hypothetical protein T28F4.2 [Caenorhabditis elegans] ref|NP_492099.1| Acid-sensing/Amiloride-Sensitive Ion Channel family member (asic-2) [Caenorhabditis elegans] sp|Q22851|DEGW_CAEEL Degenerin-like protein T28F4.2 in chromosome I 4.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 8.7E-22
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492230.2 2 34-56,445-467
T28B8.5 2 34-56,445-467
SNAP00000004693 2 34-56,411-433
GENEFINDER00000004704 2 34-56,445-467
Sequence
Target Sequence 
>C. ELEGANS:190395_AT
gattcaacggaattcgatgtgctcaccgattgtgattgccctcaaccttgcgaaatcgat
tcatatggtgtaactgtatccactgctcaatggccatccgattcatatgtgccaacagaa
tgcaatcctggtggtccatctggtccgtgggatgcttcaggagaatcgtgtcttgattgg
tataaagcaaacaccattctcattgaaatatattatgaacgaatgaacttccaagttctt
acagagtcaccagcgtatacgtttgtaaacttcatttctgatgttggcggtcaagttggt
ttgttccttggaatgagtattatatctgcaatcgaatatcttgttttgatatttcttgtc
tttttctattgttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCAACGGAATTCGATGTGCTCA 282 431 1135 Antisense
CCCTCAACCTTGCGAAATCGATTCA 455 259 1173 Antisense
TGGTGTAACTGTATCCACTGCTCAA 115 553 1200 Antisense
AATGGCCATCCGATTCATATGTGCC 14 163 1223 Antisense
AATGCAATCCTGGTGGTCCATCTGG 195 165 1253 Antisense
ATCTGGTCCGTGGGATGCTTCAGGA 609 33 1272 Antisense
ACTTCCAAGTTCTTACAGAGTCACC 325 103 1361 Antisense
ACAGAGTCACCAGCGTATACGTTTG 616 105 1375 Antisense
ACTTCATTTCTGATGTTGGCGGTCA 292 103 1403 Antisense
TGGCGGTCAAGTTGGTTTGTTCCTT 487 543 1419 Antisense
ATTTCTTGTCTTTTTCTATTGTTGT 43 17 1485 Antisense
Sequence Source Affymetrix Proprietary Database