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[190393_at] acetylcholine receptor

Description C15A7.1 /REP_DB=WormBase Gene ID /WP=CE03021 /TR=Q17999 /GB=CAA91530.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=acetylcholine receptor
Cluster 6grp_total > 82      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 4.5
(-3)
A 		4.5
(-3)
A 		18.2
(3)
A 		8.7
(-1.2)
A 		14
B 6.2
(-2.8)
A 		9.3
(-2.4)
A 		4.4
(-3)
A 		53
(3)
A 		49
C 7.2
(-2.6)
A 		10.6
(-2.3)
A 		2.7
(-3)
A 		72.4
(3)
A 		70
avr 6
(-3) 		8.1
(-2.7) 		8.4
(-2.6) 		44.7
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 11.7
(-2.1)
A 		45
(1.6)
A 		3.9
(-3)
A 		19.9
(-1.2)
A 		57.6
(3)
A 		5.2
(-2.9)
A 		54
B 4.4
(-3)
A 		4.3
(-3)
A 		5.5
(-2.8)
A 		34.7
(3)
A 		7.3
(-2.4)
A 		6.1
(-2.6)
A 		30
C 4.9
(-3)
A 		6.6
(-1.5)
A 		11.7
(3)
A 		10.6
(2)
A 		8.4
(0.1)
A 		5.5
(-2.5)
A 		7
avr 7
(-2.6) 		18.6
(1.1) 		7
(-2.6) 		21.7
(2.1) 		24.4
(3) 		5.6
(-3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.6
(-2.1)
A 		1
(-3)
A 		4.8
(3)
A 		1.3
(-2.5)
A 		4
B 2
(-2.4)
A 		2.3
(-2.2)
A 		1
(-3)
A 		11.3
(3)
A 		10
C 2.4
(-2.3)
A 		3.1
(-1.9)
A 		1
(-3)
A 		12.2
(3)
A 		11
avr 2
(-3) 		2.1
(-2.9) 		2.3
(-2.7) 		8.3
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2.2
(-2.5)
A 		16
(2.5)
A 		0.8
(-3)
A 		5
(-1.5)
A 		17.4
(3)
A 		1.3
(-2.8)
A 		17
B 1.5
(-2.8)
A 		1.4
(-2.9)
A 		1.3
(-3)
A 		9.2
(3)
A 		3
(-1.7)
A 		2.3
(-2.2)
A 		8
C 1.8
(-3)
A 		2
(-2)
A 		3
(3)
A 		2.1
(-1.5)
A 		2.7
(1.5)
A 		1.9
(-2.5)
A 		1
avr 1.8
(-2.9) 		6.5
(1.8) 		1.7
(-3) 		5.4
(0.7) 		7.7
(3) 		1.8
(-2.9) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190393_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16565
Sequence Type Exemplar sequence
Representative Public ID C15A7.1 NCBI
Target Description C15A7.1 /REP_DB=WormBase Gene ID /WP=CE03021 /TR=Q17999 /GB=CAA91530.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=acetylcholine receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077615(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077615 NCBI Caenorhabditis elegans C15A7.1 (C15A7.1) mRNA, complete cds. 11/11 None
SNAP00000010459 ENSEMBL cdna:SNAP chromosome:CEL140:X:12068471:12070472:1 9/11 None
GENEFINDER00000010462 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12068471:12070472:1 11/11 None
C15A7.1 ENSEMBL cdna:known chromosome:CEL140:X:12065225:12070472:1 gene:C15A7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12065224-12070472(+) 100.0 100.0
Public Domain and Genome References
Gene Title acetylcholine receptor
Ensembl C15A7.1
Entrez Gene 182622 Entrez gene
SwissProt Q17999 EMBL-EBI
WormBase CE03021 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91530 Hypothetical protein C15A7.1 [Caenorhabditis elegans] ref|NP_510016.1| C15A7.1 [Caenorhabditis elegans] 0.0
blast CAA91530 Hypothetical protein C15A7.1 [Caenorhabditis elegans] ref|NP_510016.1| C15A7.1 [Caenorhabditis elegans] 1.0E-148
blast NP_001022053 D2062.12 [Caenorhabditis elegans] gb|AAY44018.1| Hypothetical protein D2062.12 [Caenorhabditis elegans] 2.0E-54
blast NP_001022053 D2062.12 [Caenorhabditis elegans] gb|AAY44018.1| Hypothetical protein D2062.12 [Caenorhabditis elegans] 6.0E-49
blast NP_001022053 D2062.12 [Caenorhabditis elegans] gb|AAY44018.1| Hypothetical protein D2062.12 [Caenorhabditis elegans] 7.0E-47
blast CAE74710 Hypothetical protein CBG22525 [Caenorhabditis briggsae] 1.0E-38
blast CAE74710 Hypothetical protein CBG22525 [Caenorhabditis briggsae] 3.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.0E-35
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 8.5E-5
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.9E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510016.1 7 264-286,291-308,323-345,684-706,710-729,742-764,792-811
C15A7.1 7 264-286,291-308,323-345,684-706,710-729,742-764,792-811
SNAP00000010459 4 207-229,233-252,265-287,315-334
GENEFINDER00000010462 3 274-296,317-339,367-386
Sequence
Target Sequence 
>C. ELEGANS:190393_AT
gaatttcgatttgtgtctcccaatggttactgtaatccaaaatttgaaaatcttgaacac
cgtgcagttcgagtccacgatttacatcttatgggagttgagtccaatattttttatacg
tttgcaaatacatattttactgctgaggaagttaccggttttgaatacatggctaaaaca
gaattccgtttcaatcttatgttcaagagagtcaacaagcttctgaatgttaaactctca
attccgtcaatcttgatttctatgttcctcattatagccggactcttccctaatggttac
tcgatctttggttatgcttactgcttttttgttgaagtaatgcatggattgtatgtttcg
aaaatacttcctaacgacatcagaggaattccctatcatggagctctagcgttgtgtttt
ttgattgagaccgtgttcctcttctgttggaaagtcttttctgtctatgctagaaaccaa
aagctcttcgctcaacttccaaatgtgtttgctgacaacagagtctttgtattgaaattt
gtgttctacgttgatagagtagtgtgtgtcgcattatgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTCGATTTGTGTCTCCCAATG 638 325 1843 Antisense
TGAACACCGTGCAGTTCGAGTCCAC 569 573 1896 Antisense
GTTAAACTCTCAATTCCGTCAATCT 687 443 2071 Antisense
GTTCCTCATTATAGCCGGACTCTTC 404 439 2106 Antisense
TCTTCCCTAATGGTTACTCGATCTT 454 615 2126 Antisense
CGATCTTTGGTTATGCTTACTGCTT 683 247 2144 Antisense
GAGGAATTCCCTATCATGGAGCTCT 554 403 2225 Antisense
GGAGCTCTAGCGTTGTGTTTTTTGA 338 521 2242 Antisense
TTGATTGAGACCGTGTTCCTCTTCT 249 707 2263 Antisense
AACCAAAAGCTCTTCGCTCAACTTC 240 147 2317 Antisense
GAGTAGTGTGTGTCGCATTATGCTT 604 399 2399 Antisense
Sequence Source Affymetrix Proprietary Database