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[190387_at] Na-K-Cl cotransporter

Description R13A1.7 /REP_DB=WormBase Gene ID /WP=CE04842 /TR=Q21976 /GB=AAA81475.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=Na-K-Cl cotransporter
Cluster 6grp_total > 83      4grp_total > 50      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 8.2
(-3)
A 		18.7
(-1.8)
A 		62.1
(3)
A 		9.5
(-2.9)
A 		54
B 18.3
(-1.6)
A 		57.4
(3)
A 		15.7
(-1.9)
A 		6.2
(-3)
A 		51
C 11.2
(-3)
A 		35.3
(3)
A 		13.5
(-2.4)
A 		22.4
(-0.2)
A 		24
avr 12.6
(-3) 		37.1
(3) 		30.4
(1.4) 		12.7
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 15.3
(3)
A 		8.9
(-1.4)
A 		14.4
(2.4)
A 		8.6
(-1.6)
A 		10.9
(-0)
A 		6.6
(-3)
A 		9
B 9.2
(-3)
A 		18.8
(-1.6)
A 		25
(-0.6)
A 		33.3
(0.6)
A 		49.4
(3)
A 		11.7
(-2.6)
A 		40
C 17.3
(0.1)
A 		14.4
(-0.9)
A 		26.3
(3)
A 		7.9
(-3)
A 		14.1
(-1)
A 		8.5
(-2.8)
A 		18
avr 13.9
(-1.1) 		14
(-1.1) 		21.9
(1.9) 		16.6
(-0.1) 		24.8
(3) 		8.9
(-3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 3
(-2.4)
A 		4.1
(-1.9)
A 		16.2
(3)
A 		1.4
(-3)
A 		15
B 5.8
(-0.9)
A 		14.2
(3)
A 		3.6
(-1.9)
A 		1.3
(-3)
A 		13
C 3.7
(-3)
A 		10.2
(3)
A 		4.7
(-2.1)
A 		3.8
(-2.9)
A 		7
avr 4.2
(-1.4) 		9.5
(3) 		8.2
(1.9) 		2.2
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 2.8
(1.2)
A 		3.2
(2.6)
A 		3.1
(2.3)
A 		2.2
(-0.9)
A 		3.3
(3)
A 		1.6
(-3)
A 		2
B 3.2
(-3)
A 		6.2
(-1.9)
A 		6.1
(-2)
A 		8.8
(-1)
A 		20.1
(3)
A 		4.4
(-2.6)
A 		17
C 6.4
(2.6)
A 		4.4
(0.3)
A 		6.7
(3)
A 		1.6
(-3)
A 		4.6
(0.5)
A 		2.8
(-1.6)
A 		5
avr 4.1
(-1.9) 		4.6
(-1.4) 		5.3
(-0.8) 		4.2
(-1.8) 		9.3
(3) 		2.9
(-3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190387_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9751
Sequence Type Exemplar sequence
Representative Public ID R13A1.7 NCBI
Target Description R13A1.7 /REP_DB=WormBase Gene ID /WP=CE04842 /TR=Q21976 /GB=AAA81475.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=Na-K-Cl cotransporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068739(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068739 NCBI Caenorhabditis elegans R13A1.7 (R13A1.7) mRNA, complete cds. 11/11 None
R13A1.7 ENSEMBL cdna:known chromosome:CEL140:IV:7213211:7213513:-1 gene:R13A1.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003735 9/11 Negative Strand Matching Probes None
GENEFINDER00000003739 9/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7213216-7213519(-) 100.0 100.0
Public Domain and Genome References
Gene Title Na-K-Cl cotransporter
Ensembl R13A1.7
Entrez Gene 187852 Entrez gene
SwissProt Q21976 EMBL-EBI
WormBase CE04842 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_501140 R13A1.7 [Caenorhabditis elegans] gb|AAA81475.1| Hypothetical protein R13A1.7 [Caenorhabditis elegans] 2.0E-35
Sequence
Target Sequence 
>C. ELEGANS:190387_AT
catctctagtcattggtggcggtgcatcagtctcaacagtaaaagtaatcggtgcagtag
ttccagtgggaaccacctgatgatgggtggaaagaagacttgtgtgctcatgagagccac
tttcatggtcatcgccaccgactacgaatttgaaagacggtggtgccggagaaccggtac
cattgccggttgtcatttaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATCTCTAGTCATTGGTGGCGGTGC 85 213 21 Antisense
TCATTGGTGGCGGTGCATCAGTCTC 126 619 30 Antisense
GGTGCATCAGTCTCAACAGTAAAAG 384 501 41 Antisense
TAATCGGTGCAGTAGTTCCAGTGGG 513 635 66 Antisense
GTTCCAGTGGGAACCACCTGATGAT 406 441 80 Antisense
AGAAGACTTGTGTGCTCATGAGAGC 168 75 113 Antisense
TGAGAGCCACTTTCATGGTCATCGC 308 569 131 Antisense
TCATCGCCACCGACTACGAATTTGA 647 619 149 Antisense
GAAAGACGGTGGTGCCGGAGAACCG 158 353 172 Antisense
GCCGGAGAACCGGTACCATTGCCGG 153 283 185 Antisense
GTACCATTGCCGGTTGTCATTTAAT 591 457 197 Antisense
Sequence Source Affymetrix Proprietary Database