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[190383_s_at] NADPH-cytochrome P450

Description K10D2.6 /REP_DB=WormBase Gene ID /WP=CE02018 /TR=Q09590 /GB=AAA62544.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADPH-cytochrome P450
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 76      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 78      6grp_total > 66      4grp_total > 09      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6512
1 2 3 4 range
A 1134
(-3)
P 		1710.9
(-2.4)
P 		1674
(-2.4)
P 		6741.9
(3)
P 		5608
B 1157.3
(-3)
P 		1795.3
(-2.4)
P 		1787.9
(-2.4)
P 		7565.4
(3)
P 		6408
C 1053.3
(-3)
P 		1713.4
(-2.3)
P 		2004.6
(-2)
P 		6974.4
(3)
P 		5921
avr 1114.9
(-3) 		1739.9
(-2.4) 		1822.2
(-2.3) 		7093.9
(3) 		5979

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1744
1 2 3 4 5 6 range
A 970
(-3)
P 		1575.4
(-0.7)
P 		1447.8
(-1.2)
P 		1748
(-0)
P 		1113
(-2.4)
P 		2527.2
(3)
P 		1557
B 1466.3
(-1.3)
P 		974.7
(-3)
P 		1449.7
(-1.4)
P 		1442.5
(-1.4)
P 		1613.6
(-0.8)
P 		2713.8
(3)
P 		1739
C 1740.2
(0.6)
P 		1696.3
(0.1)
P 		1501.2
(-2.1)
P 		1562.8
(-1.4)
P 		1424.9
(-3)
P 		1954.1
(3)
P 		529
avr 1392.2
(-3) 		1415.5
(-2.8) 		1466.2
(-2.5) 		1584.4
(-1.8) 		1383.8
(-3) 		2398.4
(3) 		1015
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1264
1 2 3 4 range
A 412.9
(-2.6)
P 		371.4
(-3)
P 		437.6
(-2.4)
P 		986.9
(3)
P 		616
B 369.1
(-3)
P 		443.4
(-2.6)
P 		414.3
(-2.8)
P 		1612.2
(3)
P 		1243
C 348.7
(-3)
P 		492.6
(-2)
P 		698.7
(-0.5)
P 		1176
(3)
P 		827
avr 376.9
(-3) 		435.8
(-2.6) 		516.9
(-2) 		1258.4
(3) 		882

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 833
1 2 3 4 5 6 range
A 178.8
(-3)
P 		560.3
(2.2)
P 		315.3
(-1.1)
P 		443.3
(0.6)
P 		335.8
(-0.8)
P 		615.3
(3)
P 		437
B 510.2
(-1.4)
P 		324.4
(-3)
P 		352
(-2.8)
P 		382.1
(-2.5)
P 		655.6
(-0.1)
P 		1012
(3)
P 		688
C 645
(2.8)
P 		517.6
(0.6)
P 		382.1
(-1.8)
P 		314.3
(-3)
P 		461.1
(-0.4)
P 		654.1
(3)
P 		340
avr 444.7
(-1.6) 		467.4
(-1.3) 		349.8
(-3) 		379.9
(-2.6) 		484.2
(-1) 		760.5
(3) 		411
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190383_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6778
Sequence Type Exemplar sequence
Representative Public ID K10D2.6 NCBI
Target Description K10D2.6 /REP_DB=WormBase Gene ID /WP=CE02018 /TR=Q09590 /GB=AAA62544.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADPH-cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065702(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065702 NCBI Caenorhabditis elegans K10D2.6 (K10D2.6) mRNA, complete cds. 11/11 None
K10D2.6.1 ENSEMBL cdna:known chromosome:CEL140:III:5196644:5199634:-1 gene:K10D2.6 11/11 None
K10D2.6.2 ENSEMBL cdna:known chromosome:CEL140:III:5196644:5199636:-1 gene:K10D2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5196923-5199616(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADPH-cytochrome P450
Gene Symbol emb-8
Ensembl K10D2.6
Entrez Gene 175710 Entrez gene
SwissProt Q09590 EMBL-EBI
WormBase CE02018 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18898_S_AT NADPH-cytochrome p450 reductase, putative / NADPH-ferrihemoprotein reductase, putative at
ATH1-121501:254127_AT NADPH-cytochrome p450 reductase, putative / NADPH-ferrihemoprotein reductase, putative at
CANINE_2:CFA.14381.1.S1_AT similar to NADPH--cytochrome P450 reductase (CPR) (P450R) cfa
CANINE_2:CFA.14678.1.S1_AT similar to NADPH--cytochrome P450 reductase (CPR) (P450R) cfa
CANINE_2:CFA.14678.1.S1_S_AT similar to NADPH--cytochrome P450 reductase (CPR) (P450R) cfa
DROSOPHILA_2:1631637_A_AT Cytochrome P450 reductase dm
DROSGENOME1:141208_AT Cytochrome P450 reductase dm
CHICKEN:GGA.1736.1.S1_AT similar to MGC69029 protein gga
HG-U95AV2:858_AT P450 (cytochrome) oxidoreductase hs
HC-G110:858_AT P450 (cytochrome) oxidoreductase hs
HG-U133_PLUS_2:208928_AT P450 (cytochrome) oxidoreductase hs
HG-U133A:208928_AT P450 (cytochrome) oxidoreductase hs
HG-U133A_2:208928_AT P450 (cytochrome) oxidoreductase hs
HU35KSUBC:RC_T24094_F_AT P450 (cytochrome) oxidoreductase hs
HUGENEFL:S90469_AT P450 (cytochrome) oxidoreductase hs
U133_X3P:G9964002_3P_S_AT P450 (cytochrome) oxidoreductase hs
MOE430A:1416933_AT P450 (cytochrome) oxidoreductase mm
MOUSE430A_2:1416933_AT P450 (cytochrome) oxidoreductase mm
MOUSE430_2:1416933_AT P450 (cytochrome) oxidoreductase mm
MU11KSUBA:D17571_S_AT P450 (cytochrome) oxidoreductase mm
MG-U74AV2:99019_AT P450 (cytochrome) oxidoreductase mm
MG-U74AV2:161668_F_AT P450 (cytochrome) oxidoreductase mm
RT-U34:RC_AI137856_S_AT P450 (cytochrome) oxidoreductase rn
RG-U34A:RC_AI137856_S_AT P450 (cytochrome) oxidoreductase rn
RT-U34:M10068MRNA_S_AT P450 (cytochrome) oxidoreductase rn
RG-U34A:M10068MRNA_S_AT P450 (cytochrome) oxidoreductase rn
RG-U34A:E01524CDS_S_AT P450 (cytochrome) oxidoreductase rn
RAE230A:1368213_AT P450 (cytochrome) oxidoreductase rn
RAT230_2:1368213_AT P450 (cytochrome) oxidoreductase rn
RAE230A:1387109_AT P450 (cytochrome) oxidoreductase rn
RAT230_2:1387109_AT P450 (cytochrome) oxidoreductase rn
RT-U34:RC_AA956507_AT P450 (cytochrome) oxidoreductase rn
RG-U34A:RC_AA956507_AT P450 (cytochrome) oxidoreductase rn
YEAST_2:1776023_AT NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA62544 Hypothetical protein K10D2.6 [Caenorhabditis elegans] ref|NP_498103.1| K10D2.6 [Caenorhabditis elegans] 0.0
blast CAE60034 Hypothetical protein CBG03543 [Caenorhabditis briggsae] 0.0
blast BAB18572.1 NADPH-cytochrome P-450 reductase [Homo sapiens] 1.0E-178
Protein Domains
Database ID Description E-Value
Pfam IPR008254 EMBL-EBI Flavodoxin/nitric oxide synthase 7.2E-46
Pfam IPR001433 EMBL-EBI Oxidoreductase FAD/NAD(P)-binding 1.3E-33
Pfam IPR003097 EMBL-EBI FAD-binding 2.2E-84
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498103.1 1 5-27
K10D2.6.1 1 5-27
K10D2.6.2 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:190383_S_AT
accaccaaggacgccggatcgaaatctccagttttcgttcgtaaatcaacaatgcgtctt
ccccaccgtaccacaactcaagtcattatgattggtcccggaacaggattcgctccattc
cgtggattcctccaagatcgtcaattccacaaaaatgccggaaaggaaatcggagcaatg
catttgtattatggatgccgtcatccagatcatgattacatctacaaagatgagttggca
aagttccaagaagacgaagtgctcacacacttggtttgcgctttctccagagctcaggaa
cataagatctacgtgcaagatcgcttgtgggagactcgtgatcgtatctgggatgctatt
aacgttggagctcacgtctacatttgtggagacgcccgcaacatggctcgtgacgttcag
gcaacacttcagaagattttcagagaaatcggaggaaaatctgaaacggaagctgtcgcc
tacttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCACCAAGGACGCCGGATCGAAAT 317 83 1468 Antisense
GATTGGTCCCGGAACAGGATTCGCT 334 433 1557 Antisense
TCCATTCCGTGGATTCCTCCAAGAT 340 589 1581 Antisense
TCCTCCAAGATCGTCAATTCCACAA 659 593 1595 Antisense
AGTGCTCACACACTTGGTTTGCGCT 317 59 1725 Antisense
CGCTTTCTCCAGAGCTCAGGAACAT 463 255 1746 Antisense
GGGAGACTCGTGATCGTATCTGGGA 234 495 1796 Antisense
GGAGCTCACGTCTACATTTGTGGAG 360 521 1834 Antisense
TTTGTGGAGACGCCCGCAACATGGC 326 667 1850 Antisense
GCAACATGGCTCGTGACGTTCAGGC 704 317 1865 Antisense
TGAAACGGAAGCTGTCGCCTACTTC 660 569 1929 Antisense
Sequence Source Affymetrix Proprietary Database