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[190373_at]

Description C07A12.3 /REP_DB=WormBase Gene ID /WP=CE03971 /GEN=nhr-35 /GB=AAK39150.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 72      6grp_total > 02      4grp_total > 23      6grp_cgc > 52      6grp_high1_sub > 02_03_04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 691
1 2 3 4 range
A 886.1
(3)
P 		440.2
(-1.4)
P 		271.4
(-3)
P 		630.9
(0.5)
P 		615
B 925.7
(2.6)
P 		449.7
(-1.9)
P 		330.3
(-3)
P 		962.6
(3)
P 		632
C 907
(3)
P 		393.7
(-2.3)
P 		320.4
(-3)
P 		790.9
(1.8)
P 		587
avr 906.3
(3) 		427.9
(-1.8) 		307.4
(-3) 		794.8
(1.9) 		599

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 366
1 2 3 4 5 6 range
A 349.8
(-1.2)
P 		598.6
(3)
P 		243
(-3)
P 		412.3
(-0.1)
P 		428.2
(0.1)
P 		376.9
(-0.7)
P 		356
B 368.8
(1.8)
P 		402.9
(3)
P 		348.4
(1.1)
P 		386.1
(2.4)
P 		402.8
(3)
P 		232.6
(-3)
P 		170
C 365.6
(-1.1)
P 		324.4
(-2.5)
P 		494.7
(3)
P 		367.2
(-1.1)
P 		429.2
(0.9)
P 		308
(-3)
P 		187
avr 361.4
(-0.6) 		442
(3) 		362
(-0.5) 		388.5
(0.6) 		420.1
(2) 		305.8
(-3) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 322.6
(3)
P 		95.6
(-2.4)
P 		71
(-3)
P 		92.3
(-2.5)
P 		252
B 295.2
(3)
P 		111.1
(-2.1)
P 		76.5
(-3)
P 		205.1
(0.5)
P 		219
C 300.2
(3)
P 		113.2
(-3)
P 		111.7
(-3)
P 		133.4
(-2.3)
P 		189
avr 306
(3) 		106.6
(-2.4) 		86.4
(-3) 		143.6
(-1.4) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 160
1 2 3 4 5 6 range
A 64.5
(-2.6)
P 		212.9
(3)
P 		52.9
(-3)
P 		104.6
(-1.1)
P 		129.2
(-0.1)
P 		91.8
(-1.5)
P 		160
B 128.3
(0.3)
P 		134.1
(0.8)
P 		84.6
(-3)
P 		102.3
(-1.7)
P 		163.7
(3)
P 		86.8
(-2.8)
P 		79
C 135.5
(2.7)
P 		99
(-0.7)
P 		125.9
(1.8)
P 		73.8
(-3)
P 		138.9
(3)
P 		103.1
(-0.3)
P 		65
avr 109.4
(-0.9) 		148.7
(3) 		87.8
(-3) 		93.6
(-2.4) 		143.9
(2.5) 		93.9
(-2.4) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190373_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16285
Sequence Type Exemplar sequence
Representative Public ID C07A12.3 NCBI
Target Description C07A12.3 /REP_DB=WormBase Gene ID /WP=CE03971 /GEN=nhr-35 /GB=AAK39150.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029194(9), NM_001029195(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029194 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-35) (nhr-35) mRNA, complete cds. 9/11 None
NM_001029195 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-35) (nhr-35) mRNA, complete cds. 9/11 None
GENEFINDER00000032355 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4532661:4537228:1 9/11 None
C07A12.3 ENSEMBL cdna:known chromosome:CEL140:X:4532661:4537504:1 gene:C07A12.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032346 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4532661-4537229(+) 100.0 100.0
Public Domain and Genome References
Ensembl C07A12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39150 Nuclear hormone receptor family protein 35, isoform a [Caenorhabditis elegans] ref|NP_001024365.1| Nuclear Hormone Receptor family member (nhr-35) [Caenorhabditis elegans] sp|Q17771|NHR35_CAEEL Nuclear hormone receptor family member nhr-35 0.0
blast AAG15130 nuclear receptor NHR-35 [Caenorhabditis elegans] 0.0
blast AAR11981 nuclear receptor NHR-35 [Caenorhabditis elegans] 0.0
blast AAX88836 Nuclear hormone receptor family protein 35, isoform b [Caenorhabditis elegans] ref|NP_001024366.1| Nuclear Hormone Receptor family member (nhr-35) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.5E-36
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.3E-39
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.5E-36
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.3E-39
Sequence
Target Sequence 
>C. ELEGANS:190373_AT
acgtgactctttgtgtggcttatcgctctgttggtgcaaatgacgctttgaaacttctca
acgatctctacattccaagggcttcaaaaaccacaccacatctgaaagaatatgtggatg
gattctatttacgtgactgtgaaaaagtgatggaccagttggtggaaccaatgcgatttt
tgaagctcgacaacaaggaatttgtggctctcaaagcttgcgttcttttcaatcccgtgg
caccgggtctatccaaccatgctgttaatttggtccttaacgcaagaaggaaaattttcg
ccgcattcgaaaaatatgtaagagtgaacaaaccgctggaaaccacacgagtcggagatt
tgacatttttcattctcacaccgctttcggtaaagcttttctttagcatctattcgtacg
attttgaatacatcagatctctcactgccggcctcttgctcaaaacagacgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTGACTCTTTGTGTGGCTTATCG 118 95 950 Antisense
TGGCTTATCGCTCTGTTGGTGCAAA 282 545 965 Antisense
AACTTCTCAACGATCTCTACATTCC 410 139 1001 Antisense
AAGGGCTTCAAAAACCACACCACAT 562 159 1026 Antisense
CAAAGCTTGCGTTCTTTTCAATCCC 137 193 1161 Antisense
TTCAATCCCGTGGCACCGGGTCTAT 131 653 1177 Antisense
GGGTCTATCCAACCATGCTGTTAAT 344 497 1194 Antisense
AAACCGCTGGAAACCACACGAGTCG 671 125 1279 Antisense
TTCATTCTCACACCGCTTTCGGTAA 593 687 1318 Antisense
GAATACATCAGATCTCTCACTGCCG 381 327 1375 Antisense
CGGCCTCTTGCTCAAAACAGACGAT 20 243 1398 Antisense
Sequence Source Affymetrix Proprietary Database