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[190366_at] tubulin alpha-2 chain

Description F44F4.11 /REP_DB=WormBase Gene ID /WP=CE18680 /TR=Q20409 /GB=CAA85463.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=tubulin alpha-2 chain
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 51      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 34      6grp_total > 62      4grp_total > 57      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3810
1 2 3 4 range
A 1697.8
(-1.7)
P 		4427.1
(3)
P 		4416.2
(3)
P 		930
(-3)
P 		3497
B 1693.3
(-2.3)
P 		3059.3
(0.1)
P 		4740.1
(3)
P 		1272.7
(-3)
P 		3467
C 1553.8
(-2.1)
P 		3247.6
(1)
P 		4330.1
(3)
P 		1052.1
(-3)
P 		3278
avr 1648.3
(-2) 		3578
(1.4) 		4495.5
(3) 		1084.9
(-3) 		3411

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2036
1 2 3 4 5 6 range
A 1598
(-1.4)
P 		2239.6
(1.1)
P 		2737.3
(3)
P 		2660.7
(2.7)
P 		1653.7
(-1.2)
P 		1171.8
(-3)
P 		1566
B 1646.2
(-1.2)
P 		2194
(0.7)
P 		2878.8
(3)
P 		2240
(0.8)
P 		2147.7
(0.5)
P 		1115.8
(-3)
P 		1763
C 1785.8
(-1.2)
P 		1908
(-0.8)
P 		3151.5
(3)
P 		2540.9
(1.1)
P 		2143.5
(-0.1)
P 		1184.2
(-3)
P 		1967
avr 1676.7
(-1.2) 		2113.9
(0.3) 		2922.5
(3) 		2480.5
(1.5) 		1981.6
(-0.2) 		1157.3
(-3) 		1765
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1373
1 2 3 4 range
A 618.2
(-0.2)
P 		961
(1.9)
P 		1154.5
(3)
P 		136.1
(-3)
P 		1018
B 540
(-1.1)
P 		755.6
(0.5)
P 		1098.4
(3)
P 		271.2
(-3)
P 		827
C 514.3
(-1.5)
P 		933.7
(0.4)
P 		1509.2
(3)
P 		177.4
(-3)
P 		1332
avr 557.5
(-0.9) 		883.4
(0.9) 		1254
(3) 		194.9
(-3) 		1059

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 587
1 2 3 4 5 6 range
A 294.6
(-2.9)
P 		796.5
(3)
P 		596.1
(0.6)
P 		674.8
(1.6)
P 		499
(-0.5)
P 		285.3
(-3)
P 		511
B 572.8
(-0.9)
P 		730.3
(1.1)
P 		699.1
(0.7)
P 		593.3
(-0.7)
P 		872.6
(3)
P 		416.1
(-3)
P 		457
C 661.9
(0.9)
P 		582.2
(-0.3)
P 		802.1
(3)
P 		511
(-1.3)
P 		693.6
(1.4)
P 		396.4
(-3)
P 		406
avr 509.8
(-0.4) 		703
(3) 		699.1
(2.9) 		593
(1) 		688.4
(2.7) 		365.9
(-3) 		337
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190366_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3773
Sequence Type Exemplar sequence
Representative Public ID F44F4.11 NCBI
Target Description F44F4.11 /REP_DB=WormBase Gene ID /WP=CE18680 /TR=Q20409 /GB=CAA85463.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=tubulin alpha-2 chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063950(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063950 NCBI Caenorhabditis elegans TuBulin, Alpha family member (tba-4) (tba-4) mRNA, complete cds. 11/11 None
SNAP00000037128 ENSEMBL cdna:SNAP chromosome:CEL140:II:10919164:10920073:1 11/11 None
GENEFINDER00000037131 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10919229:10920073:1 11/11 None
F44F4.11 ENSEMBL cdna:known chromosome:CEL140:II:10918617:10920122:1 gene:F44F4.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10918625-10920070(+) 100.0 100.0
Public Domain and Genome References
Gene Title tubulin alpha-2 chain
Gene Symbol tba-4
Ensembl F44F4.11
Entrez Gene 174680 Entrez gene
SwissProt Q20409 EMBL-EBI
WormBase CE18680 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261639_AT tubulin alpha-2/alpha-4 chain (TUA2) at
CANINE:1604133_S_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFAAFFX.13667.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFAAFFX.13695.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
CANINE_2:CFAAFFX.26512.1.S1_AT similar to Tubulin alpha-2 chain (Alpha-tubulin 2) cfa
HG-U133_PLUS_2:209251_X_AT tubulin alpha 6 hs
HG-U133A_2:209251_X_AT tubulin alpha 6 hs
HG-U133A:209251_X_AT tubulin alpha 6 hs
HG-U133_PLUS_2:211750_X_AT tubulin alpha 6 hs
HG-U133A:211750_X_AT tubulin alpha 6 hs
HG-U133A_2:211750_X_AT tubulin alpha 6 hs
U133_X3P:G13436316_3P_S_AT tubulin alpha 6 hs
MOE430A:1448232_X_AT tubulin, alpha 6 mm
MOUSE430_2:1448232_X_AT tubulin, alpha 6 mm
MOUSE430A_2:1448232_X_AT tubulin, alpha 6 mm
MOE430A:1416128_AT tubulin, alpha 6 mm
MOUSE430_2:1416128_AT tubulin, alpha 6 mm
MOUSE430A_2:1416128_AT tubulin, alpha 6 mm
MU11KSUBB:MSA.13449.0_AT tubulin, alpha 6 mm
MU11KSUBB:MSA.33563.0_F_AT tubulin, alpha 6 mm
MG-U74AV2:98759_F_AT tubulin, alpha 6 mm
MG-U74AV2:101543_F_AT tubulin, alpha 6 mm
MG-U74BV2:164955_F_AT tubulin, alpha 6 mm
MU11KSUBB:MSA.27963.0_F_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU11KSUBB:MSA.34689.0_S_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBA:TC22651_F_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBA:TC22651_I_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBA:TC22653_F_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBC:TC35665_F_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBC:TC35906_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBC:TC35907_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
MU19KSUBC:TC35908_F_AT Tubulin, alpha 6, mRNA (cDNA clone MGC:30244 IMAGE:5124686) mm
RG-U34A:RC_AA892333_AT tubulin, alpha 6 (predicted) rn
RG-U34A:J00797CDS_S_AT tubulin, alpha 6 (predicted) rn
RAE230A:1367579_A_AT tubulin, alpha 6 (predicted) rn
RAT230_2:1367579_A_AT tubulin, alpha 6 (predicted) rn
YEAST_2:1778222_AT Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85463 Hypothetical protein F44F4.11 [Caenorhabditis elegans] emb|CAA19476.1| Hypothetical protein F44F4.11 [Caenorhabditis elegans] ref|NP_496351.1| TuBulin, Alpha family member (tba-4) [Caenorhabditis elegans] 0.0
blast CAE63472 Hypothetical protein CBG07939 [Caenorhabditis briggsae] 0.0
blast CAB16856 Hypothetical protein C47B2.3 [Caenorhabditis elegans] ref|NP_001021050.1| C47B2.3 [Caenorhabditis elegans] dbj|BAA03610.1| alpha-2 tubulin [Caenorhabditis elegans] sp|P34690|TBA2_CAEEL Tubulin alpha-2 chain 0.0
blast CAA85463 Hypothetical protein F44F4.11 [Caenorhabditis elegans] emb|CAA19476.1| Hypothetical protein F44F4.11 [Caenorhabditis elegans] ref|NP_496351.1| TuBulin, Alpha family member (tba-4) [Caenorhabditis elegans] 1.0E-156
blast CAB16856 Hypothetical protein C47B2.3 [Caenorhabditis elegans] ref|NP_001021050.1| C47B2.3 [Caenorhabditis elegans] dbj|BAA03610.1| alpha-2 tubulin [Caenorhabditis elegans] sp|P34690|TBA2_CAEEL Tubulin alpha-2 chain 1.0E-152
blast CAE63472 Hypothetical protein CBG07939 [Caenorhabditis briggsae] 1.0E-152
blast CAA85463 Hypothetical protein F44F4.11 [Caenorhabditis elegans] emb|CAA19476.1| Hypothetical protein F44F4.11 [Caenorhabditis elegans] ref|NP_496351.1| TuBulin, Alpha family member (tba-4) [Caenorhabditis elegans] 1.0E-143
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 1.1E-109
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.2E-78
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 1.2E-34
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.2E-78
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 2.3E-21
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.2E-78
Sequence
Target Sequence 
>C. ELEGANS:190366_AT
cactccattgatctctgccgagaaggcatatcacgaggctctgtctgtcaatgacatcac
caatagctgcttcgaaccagcaaatcagatggtcaaatgtgatccacgcaacggaaagta
catggctgtctgtcttctgtaccgcggagatgttgtgccgaaggatgtgaacactgcaat
tactgccatcaaaaccaagagatcagttcaatttgtggattggtgcccaactgggttcaa
ggttggaatcaactaccaaccaccaactgtggttccaggaggtgatctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTCCATTGATCTCTGCCGAGAAG 296 197 822 Antisense
GCATATCACGAGGCTCTGTCTGTCA 667 307 847 Antisense
ATAGCTGCTTCGAACCAGCAAATCA 234 23 884 Antisense
GGTCAAATGTGATCCACGCAACGGA 626 503 912 Antisense
AAAGTACATGGCTGTCTGTCTTCTG 85 123 936 Antisense
TTCTGTACCGCGGAGATGTTGTGCC 75 695 956 Antisense
GGATGTGAACACTGCAATTACTGCC 91 515 984 Antisense
ACTGCAATTACTGCCATCAAAACCA 494 97 994 Antisense
ACTGGGTTCAAGGTTGGAATCAACT 593 91 1051 Antisense
CTACCAACCACCAACTGTGGTTCCA 104 225 1074 Antisense
AACTGTGGTTCCAGGAGGTGATCTT 64 121 1086 Antisense
Sequence Source Affymetrix Proprietary Database