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[190364_s_at]

Description ZK270.2B /REP_DB=WormBase Gene ID /WP=CE15309 /TR=O18673 /GB=CAB05004.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Band 4.1 protein like
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 57      4grp_high_8_8 > 33      6grp_highest_nonorm > 20      6grp_total > 16      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 736
1 2 3 4 range
A 1331.5
(3)
P 		842.6
(-1.3)
P 		647.7
(-3)
P 		756
(-2)
P 		684
B 1188.8
(3)
P 		836.4
(-0.9)
P 		664.2
(-2.8)
P 		650
(-3)
P 		539
C 1250.6
(3)
P 		874.4
(-0.4)
P 		677.7
(-2.2)
P 		595.7
(-3)
P 		655
avr 1257
(3) 		851.1
(-1.1) 		663.2
(-3) 		667.2
(-3) 		594

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1650
1 2 3 4 5 6 range
A 1214.7
(2.2)
P 		1318.3
(3)
P 		520.7
(-3)
P 		705.1
(-1.6)
P 		700
(-1.7)
P 		891.1
(-0.2)
P 		798
B 1273.9
(3)
P 		954.8
(0.2)
P 		586.6
(-2.9)
P 		725.4
(-1.7)
P 		765.9
(-1.4)
P 		580.7
(-3)
P 		693
C 2170.9
(3)
P 		1018.9
(-1.4)
P 		912.9
(-1.8)
P 		680.3
(-2.7)
P 		830.8
(-2.1)
P 		595.3
(-3)
P 		1576
avr 1553.2
(3) 		1097.3
(-0.1) 		673.4
(-3) 		703.6
(-2.8) 		765.6
(-2.4) 		689
(-2.9) 		880
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 384
1 2 3 4 range
A 484.8
(3)
P 		182.9
(-1.8)
P 		169.3
(-2.1)
P 		110.7
(-3)
P 		374
B 379.1
(3)
P 		206.6
(-1.3)
P 		153.9
(-2.6)
P 		138.5
(-3)
P 		241
C 414
(3)
P 		251.4
(-0.1)
P 		236.2
(-0.4)
P 		100.5
(-3)
P 		314
avr 426
(3) 		213.6
(-1.1) 		186.5
(-1.6) 		116.6
(-3) 		309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 691
1 2 3 4 5 6 range
A 223.9
(-1.1)
P 		468.8
(3)
P 		113.4
(-3)
P 		178.8
(-1.9)
P 		211.2
(-1.3)
P 		217
(-1.3)
P 		355
B 443.2
(3)
P 		317.8
(0.5)
P 		142.4
(-3)
P 		192.1
(-2)
P 		311.2
(0.4)
P 		216.5
(-1.5)
P 		301
C 804.7
(3)
P 		310.9
(-1.4)
P 		232.3
(-2.1)
P 		136.8
(-3)
P 		268.8
(-1.8)
P 		199.3
(-2.4)
P 		668
avr 490.6
(3) 		365.8
(0.7) 		162.7
(-3) 		169.2
(-2.9) 		263.7
(-1.2) 		210.9
(-2.1) 		328
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190364_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2147
Sequence Type Exemplar sequence
Representative Public ID ZK270.2B NCBI
Target Description ZK270.2B /REP_DB=WormBase Gene ID /WP=CE15309 /TR=O18673 /GB=CAB05004.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Band 4.1 protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061199(11), NM_001026695(11), NM_061198(11), NM_061200(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061199 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) (frm-1) mRNA, complete cds. 11/11 None
NM_001026695 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) (frm-1) mRNA, complete cds. 11/11 None
NM_061198 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) (frm-1) mRNA, complete cds. 11/11 None
NM_061200 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) (frm-1) mRNA, complete cds. 11/11 None
SNAP00000023706 ENSEMBL cdna:SNAP chromosome:CEL140:I:14884514:14887681:-1 11/11 None
GENEFINDER00000023709 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14884514:14887111:-1 11/11 None
ZK270.2a.1 ENSEMBL cdna:known chromosome:CEL140:I:14883882:14905487:-1 gene:ZK270.2 11/11 A 
ZK270.2a.2 ENSEMBL cdna:known chromosome:CEL140:I:14883882:14905485:-1 gene:ZK270.2 11/11 A 
ZK270.2a.3 ENSEMBL cdna:known chromosome:CEL140:I:14883902:14905924:-1 gene:ZK270.2 11/11 None
ZK270.2d ENSEMBL cdna:known chromosome:CEL140:I:14884514:14905480:-1 gene:ZK270.2 11/11 None
ZK270.2b ENSEMBL cdna:known chromosome:CEL140:I:14884514:14905480:-1 gene:ZK270.2 11/11 None
ZK270.2e ENSEMBL cdna:known chromosome:CEL140:I:14884514:14908331:-1 gene:ZK270.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14884444-14905468(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZK270.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD91717 Hypothetical protein ZK270.2e [Caenorhabditis elegans] emb|CAD91628.1| Hypothetical protein ZK270.2e [Caenorhabditis elegans] ref|NP_001021866.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) [Caenorhabditis elegans] 0.0
blast CAB05003 Hypothetical protein ZK270.2a [Caenorhabditis elegans] emb|CAB04090.2| Hypothetical protein ZK270.2a [Caenorhabditis elegans] ref|NP_493600.2| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) [Caenorhabditis elegans] 0.0
blast CAB54513 Hypothetical protein ZK270.2d [Caenorhabditis elegans] emb|CAB54224.2| Hypothetical protein ZK270.2d [Caenorhabditis elegans] ref|NP_493601.2| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) [Caenorhabditis elegans] 0.0
blast CAB05004 Hypothetical protein ZK270.2b [Caenorhabditis elegans] emb|CAB04091.2| Hypothetical protein ZK270.2b [Caenorhabditis elegans] ref|NP_493599.2| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-1) [Caenorhabditis elegans] 0.0
blast CAE64142 Hypothetical protein CBG08758 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 1.1E-39
Pfam IPR000299 EMBL-EBI Band 4.1 3.4E-10
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Pfam IPR000299 EMBL-EBI Band 4.1 1.1E-39
Pfam IPR000299 EMBL-EBI Band 4.1 3.4E-10
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Pfam IPR008379 EMBL-EBI 4.1, C-terminal 2.2E-42
Sequence
Target Sequence 
>C. ELEGANS:190364_S_AT
ggccaaccctatacaacggtgtctacgtggcaagagacaagcgatcttccggagcaagtt
gaagtgtacactgatgagaacggacggcagatcacgcgaacagtgaagtcgtctcaagtg
aagcacacggttcaaacgcaatccttccaaaactacattgtcgacggagatcaagttcca
gtgggcgtcgtcgacgtggaaagaagtcgcgaacagctgacaccactcggacaaaaggct
tcttcgtcgacttcttctgatgtgcaaaatggtggtggtgaaaatggtggaatcgtggag
actcagaccagaacaatgacctacgaggcacaaggcggagagaattccgcgccacctgga
tgggctgaggagggtctcggcgagtacgtctcatcgaaaagtgtgactcagggcaatcga
acgatcgaaactatcacctacaagacggaaaaagacggaattgttgagacacacgttgag
catcgtgtcaccattcactctgatggcgatattgatcatgatgcagagctctcccaagcg
attctcgaagccacccaaatgaatccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCAACCCTATACAACGGTGTCTA 542 541 1318 Antisense
ACAAGCGATCTTCCGGAGCAAGTTG 117 113 1354 Antisense
GCACACGGTTCAAACGCAATCCTTC 423 315 1440 Antisense
ATCAAGTTCCAGTGGGCGTCGTCGA 58 1 1487 Antisense
AGAAGTCGCGAACAGCTGACACCAC 445 75 1519 Antisense
GACACCACTCGGACAAAAGGCTTCT 317 367 1536 Antisense
CAAAAGGCTTCTTCGTCGACTTCTT 36 191 1549 Antisense
AGGAGGGTCTCGGCGAGTACGTCTC 200 37 1685 Antisense
GTGACTCAGGGCAATCGAACGATCG 649 481 1720 Antisense
TTGAGCATCGTGTCACCATTCACTC 674 705 1793 Antisense
CTCGAAGCCACCCAAATGAATCCAG 50 233 1861 Antisense
Sequence Source Affymetrix Proprietary Database