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[190356_at]

Description R166.5 /REP_DB=WormBase Gene ID /WP=CE03584 /TR=Q22005 /GB=CAA90665.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_high1_10_10 > 86      4grp_high_8_8 > 06      6grp_total > 76      4grp_total > 02      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 622
1 2 3 4 range
A 136.6
(-2.6)
P 		90.7
(-3)
P 		142.9
(-2.5)
P 		712.9
(3)
P 		622
B 110.7
(-2.8)
P 		97.8
(-3)
M 		126
(-2.7)
P 		595.2
(3)
P 		497
C 99.9
(-2.9)
P 		95.3
(-3)
P 		137.6
(-2.5)
P 		588.3
(3)
P 		493
avr 115.7
(-2.8) 		94.6
(-3) 		135.5
(-2.5) 		632.1
(3) 		538

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 54.8
(-2.4)
A 		45.1
(-3)
A 		97.3
(0.2)
P 		91.4
(-0.1)
P 		114.4
(1.3)
A 		142.1
(3)
P 		97
B 93.9
(-1.9)
P 		70.2
(-3)
P 		92.4
(-2)
P 		89.8
(-2.1)
P 		93.8
(-1.9)
P 		199.7
(3)
P 		130
C 72.9
(-1.1)
P 		35.3
(-3)
A 		85.6
(-0.4)
P 		101.4
(0.4)
P 		86.6
(-0.4)
P 		152.4
(3)
P 		117
avr 73.9
(-1.8) 		50.2
(-3) 		91.8
(-0.8) 		94.2
(-0.7) 		98.3
(-0.5) 		164.7
(3) 		115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 49.7
(-0.9)
P 		19.7
(-3)
P 		37.4
(-1.7)
P 		104.3
(3)
P 		85
B 35.3
(-2.3)
P 		24.1
(-3)
M 		29.2
(-2.7)
P 		126.8
(3)
P 		103
C 33.1
(-2.5)
P 		27.4
(-3)
P 		47.9
(-1.3)
P 		99.2
(3)
P 		72
avr 39.4
(-1.9) 		23.7
(-3) 		38.2
(-2) 		110.1
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 10.1
(-3)
A 		16
(-1.6)
A 		21.2
(-0.3)
P 		23.2
(0.2)
P 		34.5
(3)
A 		34.6
(3)
P 		25
B 32.7
(-1.8)
P 		23.4
(-2.9)
P 		22.4
(-3)
P 		23.8
(-2.8)
P 		38.1
(-1.2)
P 		74.5
(3)
P 		52
C 27
(-0.6)
P 		10.8
(-3)
A 		21.8
(-1.4)
P 		20.4
(-1.6)
P 		28
(-0.4)
P 		51
(3)
P 		40
avr 23.3
(-1.9) 		16.7
(-3) 		21.8
(-2.2) 		22.5
(-2.1) 		33.5
(-0.3) 		53.4
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190356_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3000
Sequence Type Exemplar sequence
Representative Public ID R166.5 NCBI
Target Description R166.5 /REP_DB=WormBase Gene ID /WP=CE03584 /TR=Q22005 /GB=CAA90665.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063871(11), NM_182124(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063871 NCBI Caenorhabditis elegans R166.5a (R166.5) mRNA, complete cds. 11/11 A 
NM_182124 NCBI Caenorhabditis elegans R166.5b (R166.5) mRNA, complete cds. 11/11 A 
SNAP00000017515 ENSEMBL cdna:SNAP chromosome:CEL140:II:10544267:10547627:-1 11/11 None
GENEFINDER00000017521 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10544267:10547627:-1 11/11 None
R166.5a ENSEMBL cdna:known chromosome:CEL140:II:10542337:10548820:-1 gene:R166.5 11/11 A 
R166.5b ENSEMBL cdna:known chromosome:CEL140:II:10544267:10554954:-1 gene:R166.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10544263-10548603(-) 100.0 100.0
Public Domain and Genome References
Ensembl R166.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90665 Hypothetical protein R166.5a [Caenorhabditis elegans] ref|NP_496272.1| R166.5a [Caenorhabditis elegans] 0.0
blast CAD59152 Hypothetical protein R166.5b [Caenorhabditis elegans] ref|NP_871924.1| R166.5b [Caenorhabditis elegans] 0.0
blast CAE59656 Hypothetical protein CBG03074 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-60
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-64
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 5.0E-70
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 3.0E-70
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 2.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Pfam IPR000719 EMBL-EBI Protein kinase 5.3E-84
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-76
Sequence
Target Sequence 
>C. ELEGANS:190356_AT
ccatccaatctcattaaccgcaaggattcggcacgtgatgttcaacaaatgtccgagcac
tttaatctgatgaaccgacttgcggacactcgtctttcggcgagattcgacaataaaatg
acgatgagcgagtgtggaagtgacttgggaactgctactattcatggtgacggatccttc
ctatccatgggtggcgaaccttttggcactttcccacgaaagaaatctgttggaattgcc
atcgaaaaatcacgaagtggagagttcacaccgccaatcagtcgtgcttctcctacaact
ccacctccatcgatgctcaatttgagcgaagatcttactgattcaccggtcaaaagaaga
tctgccgacgattcgggaaccttttcgctcttctctccagcatcatcaaacgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATCCAATCTCATTAACCGCAAGG 405 271 1687 Antisense
GCAAGGATTCGGCACGTGATGTTCA 325 319 1706 Antisense
ATGTCCGAGCACTTTAATCTGATGA 700 47 1735 Antisense
GGACACTCGTCTTTCGGCGAGATTC 572 525 1770 Antisense
TTCATGGTGACGGATCCTTCCTATC 259 689 1847 Antisense
CATGGGTGGCGAACCTTTTGGCACT 28 209 1872 Antisense
AAGTGGAGAGTTCACACCGCCAATC 244 159 1941 Antisense
CACCTCCATCGATGCTCAATTTGAG 479 201 1988 Antisense
GAAGATCTTACTGATTCACCGGTCA 487 337 2014 Antisense
GATCTGCCGACGATTCGGGAACCTT 143 415 2045 Antisense
TCTTCTCTCCAGCATCATCAAACGG 453 617 2075 Antisense
Sequence Source Affymetrix Proprietary Database