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[190345_s_at]

Description Y71H10A.1 /REP_DB=WormBase Gene ID /WP=CE28266 /TR=Q9TZL8 /GB=AAC67416.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=phosphofructokinase
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 0      6grp_total > 06      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1814
1 2 3 4 range
A 2334.5
(3)
P 		1589.4
(0.3)
P 		1376.9
(-0.4)
P 		666.3
(-3)
P 		1668
B 2036.5
(3)
P 		1734.6
(1.7)
P 		1331.1
(-0.1)
P 		649.6
(-3)
P 		1387
C 2282.3
(3)
P 		1870.5
(1.6)
P 		1611.7
(0.7)
P 		520.7
(-3)
P 		1762
avr 2217.8
(3) 		1731.5
(1.2) 		1439.9
(0.1) 		612.2
(-3) 		1606

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1253
1 2 3 4 5 6 range
A 1770
(3)
P 		1660.5
(1.9)
P 		1322.1
(-1.4)
P 		1155.4
(-3)
P 		1287.3
(-1.7)
P 		1299.3
(-1.6)
P 		615
B 2129.3
(3)
P 		1694.8
(-0.1)
P 		1552.3
(-1.1)
P 		1489.3
(-1.5)
P 		1284.4
(-2.9)
P 		1276.9
(-3)
P 		852
C 2388.8
(3)
P 		1540.8
(-1.1)
P 		1746.5
(-0.1)
P 		1135.9
(-3)
P 		1593.7
(-0.8)
P 		1219.5
(-2.6)
P 		1253
avr 2096
(3) 		1632
(-0.3) 		1540.3
(-1) 		1260.2
(-3) 		1388.5
(-2.1) 		1265.2
(-3) 		836
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 762
1 2 3 4 range
A 850
(3)
P 		345
(-1)
P 		360
(-0.9)
P 		97.5
(-3)
P 		753
B 649.4
(3)
P 		428.4
(0.4)
P 		308.4
(-1)
P 		138.4
(-3)
P 		511
C 755.5
(3)
P 		537.8
(1)
P 		561.7
(1.3)
P 		87.8
(-3)
P 		668
avr 751.6
(3) 		437.1
(0.1) 		410
(-0.2) 		107.9
(-3) 		644

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 657
1 2 3 4 5 6 range
A 326.3
(-2.2)
P 		590.6
(3)
P 		287.9
(-3)
P 		293
(-2.9)
P 		388.5
(-1)
P 		316.3
(-2.4)
P 		303
B 740.9
(3)
P 		564.1
(0.1)
P 		376.9
(-3)
P 		394.5
(-2.7)
P 		521.8
(-0.6)
P 		476.1
(-1.4)
P 		364
C 885.4
(3)
P 		470.1
(-0.8)
P 		444.5
(-1)
P 		228.4
(-3)
P 		515.7
(-0.4)
P 		408.2
(-1.4)
P 		657
avr 650.9
(3) 		541.6
(1.1) 		369.8
(-1.9) 		305.3
(-3) 		475.3
(-0) 		400.2
(-1.4) 		346
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190345_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18312
Sequence Type Exemplar sequence
Representative Public ID Y71H10A.1 NCBI
Target Description Y71H10A.1 /REP_DB=WormBase Gene ID /WP=CE28266 /TR=Q9TZL8 /GB=AAC67416.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=phosphofructokinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171643(11), NM_171644(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171643 NCBI Caenorhabditis elegans Y71H10A.1a (phosphofructokinase) mRNA, complete cds. 11/11 None
NM_171644 NCBI Caenorhabditis elegans Y71H10A.1b (phosphofructokinase) mRNA, complete cds. 11/11 None
SNAP00000039283 ENSEMBL cdna:SNAP chromosome:CEL140:X:2940881:2944279:1 11/11 None
GENEFINDER00000039287 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2940881:2945206:1 11/11 None
Y71H10A.1a ENSEMBL cdna:known chromosome:CEL140:X:2940863:2945471:1 gene:Y71H10A.1 11/11 A 
Y71H10A.1b.2 ENSEMBL cdna:known chromosome:CEL140:X:2940863:2945445:1 gene:Y71H10A.1 11/11 None
Y71H10A.1b.1 ENSEMBL cdna:known chromosome:CEL140:X:2941320:2945445:1 gene:Y71H10A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2940880-2945206(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y71H10A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC67416 Hypothetical protein Y71H10A.1a [Caenorhabditis elegans] ref|NP_741738.1| Y71H10A.1a [Caenorhabditis elegans] 0.0
blast CAE58813 Hypothetical protein CBG02020 [Caenorhabditis briggsae] 0.0
blast AAL27269 Hypothetical protein Y71H10A.1b [Caenorhabditis elegans] ref|NP_741739.2| Y71H10A.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000023 EMBL-EBI Phosphofructokinase 1.0E-126
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.6E-20
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.4E-39
Pfam IPR000023 EMBL-EBI Phosphofructokinase 7.1E-99
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.6E-20
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.4E-39
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.6E-20
Pfam IPR000023 EMBL-EBI Phosphofructokinase 2.4E-39
Sequence
Target Sequence 
>C. ELEGANS:190345_S_AT
gatactgtggttaccttgccactctttctgcgttgagctccggtgcggataatgcgtaca
tttttgaagagccattcaccgttcaagacctctccgatgatgttgatgtgattctctcaa
agatggaagttggagcgaaacgctacttggttgtcagaaatgagtgggccgacaagaacc
tcaccacagattttgtgcagaatctttttgattctgaaggaaagaagaacttcaccacaa
gagtcaatgttctcggacatgttcaacagggtggatctccaacgccgttcgacagaaata
tgggaaccaagctggccgctcgtgctttggagtttttgctcattcagctcaaggaaaatt
tgacggccgataacaaggtgatcgccaagtcagcccatactgcgacgcttcttggactca
aaggacgaaaggtggtgttcacgccggtgcaagatctcaagaaggagaccgattttgagc
atcgtcttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACTGTGGTTACCTTGCCACTCT 691 423 1883 Antisense
AAGACCTCTCCGATGATGTTGATGT 31 155 1967 Antisense
GAGCGAAACGCTACTTGGTTGTCAG 128 387 2015 Antisense
CGACAAGAACCTCACCACAGATTTT 109 251 2052 Antisense
CAACAGGGTGGATCTCCAACGCCGT 607 187 2146 Antisense
AATATGGGAACCAAGCTGGCCGCTC 207 173 2179 Antisense
GGAGTTTTTGCTCATTCAGCTCAAG 683 517 2211 Antisense
ATCGCCAAGTCAGCCCATACTGCGA 303 37 2263 Antisense
GCGACGCTTCTTGGACTCAAAGGAC 431 289 2284 Antisense
TGTTCACGCCGGTGCAAGATCTCAA 624 561 2318 Antisense
GACCGATTTTGAGCATCGTCTTCCA 500 381 2349 Antisense
Sequence Source Affymetrix Proprietary Database