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[190341_at]

Description C27B7.1 /REP_DB=WormBase Gene ID /WP=CE27804 /GEN=spr-2 /TR=Q18240 /GB=CAA90979.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=SET protein
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 27      4grp_high_8_8 > 56      6grp_highest_nonorm > 88      6grp_total > 76      4grp_total > 63      6grp_highest_sub > In      6grp_cgc > 57      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 987.9
(-3)
P 		1147.2
(3)
P 		1116.8
(1.9)
P 		1066.3
(-0)
P 		159
B 1075.4
(3)
P 		919.8
(-3)
P 		988
(-0.4)
P 		959.7
(-1.5)
P 		156
C 1084.2
(3)
P 		957.8
(-3)
P 		1064.7
(2.1)
P 		997
(-1.1)
P 		126
avr 1049.2
(2.1) 		1008.3
(-2.9) 		1056.5
(3) 		1007.7
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 919
1 2 3 4 5 6 range
A 1024.7
(-3)
P 		1165.3
(-1.6)
P 		1039.5
(-2.9)
P 		1260.4
(-0.7)
P 		1262.5
(-0.7)
P 		1641.1
(3)
P 		616
B 860.5
(-3)
P 		961.8
(-2.2)
P 		1449.7
(1.4)
P 		1200.6
(-0.5)
P 		1406.9
(1.1)
P 		1665.4
(3)
P 		805
C 911
(-3)
P 		915.3
(-3)
P 		1326.5
(-0.1)
P 		1227.7
(-0.8)
P 		1166.7
(-1.2)
P 		1779.9
(3)
P 		869
avr 932.1
(-3) 		1014.1
(-2.4) 		1271.9
(-0.3) 		1229.6
(-0.7) 		1278.7
(-0.3) 		1695.5
(3) 		763
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 215
1 2 3 4 range
A 359.7
(3)
P 		249
(-0.3)
P 		291.9
(1)
P 		156.1
(-3)
P 		204
B 342.9
(3)
P 		227.2
(-2)
P 		228.9
(-1.9)
P 		204.5
(-3)
P 		138
C 358.9
(2.6)
P 		275.4
(0.2)
P 		371.1
(3)
P 		168.1
(-3)
P 		203
avr 353.8
(3) 		250.5
(-0.5) 		297.3
(1.1) 		176.2
(-3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 432
1 2 3 4 5 6 range
A 188.9
(-3)
P 		414.5
(3)
P 		226.4
(-2)
P 		319.7
(0.5)
P 		381
(2.1)
P 		399.6
(2.6)
P 		226
B 299.4
(-3)
P 		320.1
(-2.6)
P 		352
(-2)
P 		318
(-2.7)
P 		571.6
(2.1)
P 		621
(3)
P 		322
C 337.7
(-1.4)
P 		279.3
(-2.4)
P 		337.6
(-1.4)
P 		246.9
(-3)
P 		377.5
(-0.8)
P 		595.8
(3)
P 		349
avr 275.3
(-3) 		338
(-1.6) 		305.3
(-2.3) 		294.9
(-2.6) 		443.4
(0.8) 		538.8
(3) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190341_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8687
Sequence Type Exemplar sequence
Representative Public ID C27B7.1 NCBI
Target Description C27B7.1 /REP_DB=WormBase Gene ID /WP=CE27804 /GEN=spr-2 /TR=Q18240 /GB=CAA90979.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=SET protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026162(11), NM_001026161(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026162 NCBI Caenorhabditis elegans Suppressor of PResenilin defect family member (spr-2) (spr-2) mRNA, complete cds. 11/11 None
NM_001026161 NCBI Caenorhabditis elegans Suppressor of PResenilin defect family member (spr-2) (spr-2) mRNA, complete cds. 10/11 None
SNAP00000019239 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8891457:8892520:1 11/11 None
GENEFINDER00000019256 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8891457:8892520:1 11/11 None
C27B7.1b ENSEMBL cdna:known chromosome:CEL140:IV:8891452:8892710:1 gene:C27B7.1 11/11 None
C27B7.1a ENSEMBL cdna:known chromosome:CEL140:IV:8891457:8892520:1 gene:C27B7.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8891462-8892526(+) 88.22 100.0
Public Domain and Genome References
Ensembl C27B7.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE46661 Hypothetical protein C27B7.1b [Caenorhabditis elegans] ref|NP_001021333.1| Suppressor of PResenilin defect family member (spr-2) [Caenorhabditis elegans] pir||T19503 hypothetical protein C27B7.1 - Caenorhabditis elegans 1.0E-136
blast CAA90979 Hypothetical protein C27B7.1a [Caenorhabditis elegans] ref|NP_001021332.1| Suppressor of PResenilin defect family member (spr-2) [Caenorhabditis elegans] gb|AAG42102.1| suppressor of presenilin 2 [Caenorhabditis elegans] sp|Q18240|SPR2_CAEEL Suppressor of presenilin 1.0E-136
blast CAA90979 Hypothetical protein C27B7.1a [Caenorhabditis elegans] ref|NP_001021332.1| Suppressor of PResenilin defect family member (spr-2) [Caenorhabditis elegans] gb|AAG42102.1| suppressor of presenilin 2 [Caenorhabditis elegans] sp|Q18240|SPR2_CAEEL Suppressor of presenilin 1.0E-134
blast CAE46661 Hypothetical protein C27B7.1b [Caenorhabditis elegans] ref|NP_001021333.1| Suppressor of PResenilin defect family member (spr-2) [Caenorhabditis elegans] pir||T19503 hypothetical protein C27B7.1 - Caenorhabditis elegans 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR002164 EMBL-EBI Nucleosome assembly protein (NAP) 1.6E-81
Pfam IPR002164 EMBL-EBI Nucleosome assembly protein (NAP) 3.5E-82
Sequence
Target Sequence 
>C. ELEGANS:190341_AT
gtatcatctgcaatctgagtcaccgagcaccgaaattaccgaaatcgagtggaaggagaa
caagaaaccgccattcaagccggaagacggagattcagctcacacgtttcttgaatggct
cacgtacgcagcactaccagacagtgatgagattgctgaagtgatcaaagacgatctgta
cgtcaatcctttgcagtactacgtgatgcccgacatgcaggaagttgaagaagacgatat
cgaagactttctgaatgaggagcgcggtgtagatgagaatggacaaaggattcctcgtcg
gaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATCATCTGCAATCTGAGTCACCG 455 453 435 Antisense
GAGTCACCGAGCACCGAAATTACCG 470 387 451 Antisense
GAAACCGCCATTCAAGCCGGAAGAC 359 357 498 Antisense
GACGGAGATTCAGCTCACACGTTTC 279 377 520 Antisense
GTTTCTTGAATGGCTCACGTACGCA 451 447 540 Antisense
ACGTACGCAGCACTACCAGACAGTG 247 93 556 Antisense
ACGATCTGTACGTCAATCCTTTGCA 367 93 605 Antisense
GTCAATCCTTTGCAGTACTACGTGA 236 463 616 Antisense
TACTACGTGATGCCCGACATGCAGG 576 643 631 Antisense
GACTTTCTGAATGAGGAGCGCGGTG 699 371 679 Antisense
GGACAAAGGATTCCTCGTCGGAACA 124 525 715 Antisense
Sequence Source Affymetrix Proprietary Database