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[190339_at]

Description F14B8.1 /REP_DB=WormBase Gene ID /WP=CE02644 /TR=Q19444 /GB=AAA68274.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=NA(+)-H(+) antiporter
Cluster 6grp_total > 27      4grp_total > 45      6grp_cgc > 04      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 312.2
(3)
P 		195.5
(-3)
A 		268.2
(0.7)
A 		238.6
(-0.8)
P 		117
B 284.4
(3)
A 		266.8
(2)
A 		181.9
(-3)
A 		184.5
(-2.8)
P 		103
C 293.1
(3)
P 		293
(3)
A 		201.3
(-3)
A 		220.2
(-1.8)
P 		92
avr 296.6
(3) 		251.8
(-0.3) 		217.1
(-2.8) 		214.4
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 317
1 2 3 4 5 6 range
A 354.6
(3)
P 		238.3
(0)
M 		119.1
(-3)
A 		234.4
(-0.1)
M 		204.2
(-0.8)
P 		259.7
(0.6)
P 		236
B 346
(3)
P 		295.5
(1.4)
P 		207
(-1.5)
A 		158.8
(-3)
A 		202.5
(-1.6)
P 		214.9
(-1.2)
P 		187
C 436
(3)
P 		296.1
(-0.2)
P 		228.7
(-1.8)
A 		177
(-3)
A 		239.8
(-1.5)
P 		232.2
(-1.7)
P 		259
avr 378.9
(3) 		276.6
(-0.2) 		184.9
(-3) 		190.1
(-2.8) 		215.5
(-2.1) 		235.6
(-1.4) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 113.7
(3)
P 		42.4
(-2.4)
A 		70.1
(-0.3)
A 		34.9
(-3)
P 		79
B 90.7
(3)
A 		65.9
(0.1)
A 		42.2
(-2.7)
A 		39.3
(-3)
P 		51
C 97
(3)
P 		84.3
(1.7)
A 		70.2
(0.3)
A 		37.1
(-3)
P 		60
avr 100.5
(3) 		64.2
(-0.4) 		60.8
(-0.8) 		37.1
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 65.4
(1)
P 		84.7
(3)
M 		25.9
(-3)
A 		59.4
(0.4)
M 		61.6
(0.6)
P 		63.2
(0.8)
P 		59
B 120.4
(3)
P 		98.3
(1.3)
P 		50.3
(-2.4)
A 		42
(-3)
A 		82.3
(0.1)
P 		80.1
(-0.1)
P 		78
C 161.6
(3)
P 		90.3
(-0.4)
P 		58.2
(-1.9)
A 		35.6
(-3)
A 		77.6
(-1)
P 		77.7
(-1)
P 		126
avr 115.8
(3) 		91.1
(0.9) 		44.8
(-3) 		45.7
(-2.9) 		73.8
(-0.5) 		73.7
(-0.6) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190339_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17179
Sequence Type Exemplar sequence
Representative Public ID F14B8.1 NCBI
Target Description F14B8.1 /REP_DB=WormBase Gene ID /WP=CE02644 /TR=Q19444 /GB=AAA68274.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=NA(+)-H(+) antiporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029391(11), NM_001029392(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029391 NCBI Caenorhabditis elegans Na/H eXchanger family member (nhx-4) (nhx-4) mRNA, complete cds. 11/11 None
NM_001029392 NCBI Caenorhabditis elegans Na/H eXchanger family member (nhx-4) (nhx-4) mRNA, complete cds. 11/11 None
SNAP00000027540 ENSEMBL cdna:SNAP chromosome:CEL140:X:6917281:6920456:1 11/11 None
GENEFINDER00000027545 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6917281:6920456:1 11/11 None
F14B8.1a ENSEMBL cdna:known chromosome:CEL140:X:6914241:6921077:1 gene:F14B8.1 11/11 None
F14B8.1b ENSEMBL cdna:known chromosome:CEL140:X:6917280:6920687:1 gene:F14B8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6917280-6920456(+) 100.0 100.0
Public Domain and Genome References
Ensembl F14B8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM48522 Na/h exchanger protein 4, isoform a [Caenorhabditis elegans] ref|NP_001024562.1| Na/H eXchanger family member (nhx-4) [Caenorhabditis elegans] gb|AAM18104.1| putative Na-H exchanger isoform 4a [Caenorhabditis elegans] 0.0
blast AAA68274 Na/h exchanger protein 4, isoform b [Caenorhabditis elegans] ref|NP_001024563.1| Na/H eXchanger family member (nhx-4) [Caenorhabditis elegans] gb|AAM18105.1| putative Na-H exchanger isoform 4b [Caenorhabditis elegans] 0.0
blast CAE65818 Hypothetical protein CBG10932 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.0E-126
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024562.1 11 7-29,101-123,130-147,157-174,187-209,219-241,299-321,349-371,378-400,415-437,480-502
NP_001024563.1 10 36-58,65-82,92-109,122-144,154-176,234-256,284-306,313-335,350-372,415-437
F14B8.1a 11 7-29,101-123,130-147,157-174,187-209,219-241,299-321,349-371,378-400,415-437,480-502
F14B8.1b 10 36-58,65-82,92-109,122-144,154-176,234-256,284-306,313-335,350-372,415-437
SNAP00000027540 10 36-58,65-82,92-109,122-144,154-176,234-256,284-306,313-335,350-372,415-437
GENEFINDER00000027545 10 36-58,65-82,92-109,122-144,154-176,234-256,284-306,313-335,350-372,415-437
Sequence
Target Sequence 
>C. ELEGANS:190339_AT
gcaacctgggacttccaaaagccgtcagtaccgctcttcgtattggtgaacgcagtcata
ctgatcttcaacgccacggagaagtctcccacaccccagcgccatccgtcgggcgaccaa
ccattgctccattcgatttcggaaatatggaagaagagaacaaccgtcttccatccaccg
gtagacgtactccagttgccagaagccaaactcgctactctcgtcatttcatctatgcta
ttccaaatgagactgataactacttgacctgtccagtgagcgcatccaaacgtcctccat
ttgacccatctcaagtctatcaacaccaaaacaccatcagtaaccctggagacgatcgtg
ctcgttgctataaccgtcgccacaactcgatgagcggagccgacgagatggaactcaaac
aacttaatcagacagttcagaaaaaactttcccgcaccatcacaagtggaacaaagcagt
ttgttgtgaaccccgatgaagcaatccgtgaagagtctccagccactattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACCTGGGACTTCCAAAAGCCGT 571 317 1478 Antisense
TCAGTACCGCTCTTCGTATTGGTGA 663 623 1502 Antisense
TCATACTGATCTTCAACGCCACGGA 406 619 1533 Antisense
AACCATTGCTCCATTCGATTTCGGA 8 147 1596 Antisense
GTACTCCAGTTGCCAGAAGCCAAAC 250 459 1664 Antisense
CTACTCTCGTCATTTCATCTATGCT 284 227 1692 Antisense
ACTACTTGACCTGTCCAGTGAGCGC 290 99 1736 Antisense
GACCCATCTCAAGTCTATCAACACC 536 381 1780 Antisense
AACCCTGGAGACGATCGTGCTCGTT 674 145 1819 Antisense
CCGTCGCCACAACTCGATGAGCGGA 417 265 1851 Antisense
CGTGAAGAGTCTCCAGCCACTATTC 577 243 1984 Antisense
Sequence Source Affymetrix Proprietary Database