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[190323_s_at] alpha 1,2, mannosidase

Description C52E4.5 /REP_DB=WormBase Gene ID /WP=CE08947 /TR=Q18788 /GB=CAB01415.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha 1,2, mannosidase
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 11      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 42      6grp_total > 13      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 512
1 2 3 4 range
A 916.4
(1.9)
P 		895.3
(1.6)
P 		990.2
(3)
P 		592.4
(-3)
P 		398
B 986.1
(1.4)
P 		877.8
(0)
P 		1104.6
(3)
P 		649.6
(-3)
P 		455
C 868.6
(0.2)
P 		863.7
(0.1)
P 		1059.7
(3)
P 		649.5
(-3)
P 		410
avr 923.7
(1.2) 		878.9
(0.5) 		1051.5
(3) 		630.5
(-3) 		421

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 708
1 2 3 4 5 6 range
A 1007.2
(2.9)
P 		1018.8
(3)
P 		911.1
(1.8)
P 		902.2
(1.7)
P 		784.1
(0.5)
P 		461.6
(-3)
P 		557
B 1010.9
(2.5)
P 		1051.3
(3)
P 		1008.6
(2.5)
P 		838.2
(0.5)
P 		702.8
(-1.1)
P 		546.6
(-3)
P 		505
C 1169.2
(3)
P 		940.6
(0.6)
P 		865.4
(-0.1)
P 		1009.4
(1.3)
P 		694.4
(-1.9)
P 		590.2
(-3)
P 		579
avr 1062.4
(3) 		1003.6
(2.3) 		928.4
(1.5) 		916.6
(1.3) 		727.1
(-0.8) 		532.8
(-3) 		530
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 333.7
(3)
P 		194.4
(-0.4)
P 		258.9
(1.2)
P 		86.7
(-3)
P 		247
B 314.5
(3)
P 		216.8
(-0.3)
P 		256
(1)
P 		138.4
(-3)
P 		176
C 287.5
(1.1)
P 		248.3
(0.2)
P 		369.3
(3)
P 		109.5
(-3)
P 		260
avr 311.9
(3) 		219.8
(0.2) 		294.7
(2.5) 		111.5
(-3) 		200

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 185.7
(-1.2)
P 		362.3
(3)
P 		198.4
(-0.9)
P 		228.8
(-0.2)
P 		236.6
(-0)
P 		112.4
(-3)
P 		250
B 351.7
(3)
P 		349.9
(2.9)
P 		244.9
(-1.3)
P 		222
(-2.3)
P 		285.6
(0.3)
P 		203.8
(-3)
P 		148
C 433.4
(3)
P 		287
(-0.7)
P 		220.2
(-2.4)
P 		203
(-2.9)
P 		224.7
(-2.3)
P 		197.6
(-3)
P 		236
avr 323.6
(2.6) 		333.1
(3) 		221.2
(-1.1) 		217.9
(-1.3) 		249
(-0.1) 		171.3
(-3) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190323_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13287
Sequence Type Exemplar sequence
Representative Public ID C52E4.5 NCBI
Target Description C52E4.5 /REP_DB=WormBase Gene ID /WP=CE08947 /TR=Q18788 /GB=CAB01415.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=alpha 1,2, mannosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073605 NCBI Caenorhabditis elegans C52E4.5 (C52E4.5) mRNA, complete cds. 11/11 None
SNAP00000019787 ENSEMBL cdna:SNAP chromosome:CEL140:V:11985731:11988063:-1 11/11 None
GENEFINDER00000019794 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11985731:11988063:-1 11/11 None
C52E4.5 ENSEMBL cdna:known chromosome:CEL140:V:11985503:11988072:-1 gene:C52E4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11985730-11988063(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha 1,2, mannosidase
Ensembl C52E4.5
Entrez Gene 179642 Entrez gene
SwissProt Q18788 EMBL-EBI
WormBase CE08947 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260520_AT mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative at
CANINE_2:CFAAFFX.15501.1.S1_AT similar to Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (Processing alpha-1,2-mannosidase IB) (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A member 2) cfa
DROSOPHILA_2:1629744_A_AT Mannosidase I dm
HG-U133A:217920_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133A_2:217920_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:217920_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:217282_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133A_2:217282_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133A:217282_AT mannosidase, alpha, class 1A, member 2 hs
HU35KSUBA:R36410_AT mannosidase, alpha, class 1A, member 2 hs
HU35KSUBB:RC_H97940_AT mannosidase, alpha, class 1A, member 2 hs
HU35KSUBC:RC_H01428_AT mannosidase, alpha, class 1A, member 2 hs
HU35KSUBC:RC_AA235544_AT mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS2.367638.1.S1_3P_S_AT mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.2.S1_3P_AT mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.0.S2_3P_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:1552481_S_AT mannosidase, alpha, class 1A, member 2 hs
HG-U95AV2:41298_AT mannosidase, alpha, class 1A, member 2 hs
HG-U95B:44094_AT mannosidase, alpha, class 1A, member 2 hs
HG-U95C:63870_R_AT mannosidase, alpha, class 1A, member 2 hs
HG-U133A:217921_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133A_2:217921_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:217921_AT Mannosidase, alpha, class 1A, member 2 hs
HG-FOCUS:217922_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133A_2:217922_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:217922_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133A:217922_AT Mannosidase, alpha, class 1A, member 2 hs
HU35KSUBB:RC_Z39056_AT Mannosidase, alpha, class 1A, member 2 hs
HU35KSUBB:RC_R22905_AT Mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.0.S4_3P_AT Mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.0.S3_3P_X_AT Mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.0.S3_3P_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U95B:49428_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U95C:50451_R_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U95C:59356_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133A_2:214242_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133_PLUS_2:214242_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U133A:214242_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U95AV2:41297_AT Mannosidase, alpha, class 1A, member 2 hs
HG-U95E:71924_AT Mannosidase, alpha, class 1A, member 2 hs
HU35KSUBB:RC_T56804_AT Mannosidase, alpha, class 1A, member 2 hs
HU35KSUBD:RC_AA251240_AT Mannosidase, alpha, class 1A, member 2 hs
U133_X3P:HS.239114.1.A1_3P_AT Mannosidase, alpha, class 1A, member 2 hs
MU11KSUBA:U03458_S_AT mannosidase, alpha, class 1A, member 2 mm
MG-U74AV2:92650_AT mannosidase, alpha, class 1A, member 2 mm
MG-U74BV2:105228_AT mannosidase, alpha, class 1A, member 2 mm
MG-U74BV2:115917_AT mannosidase, alpha, class 1A, member 2 mm
MG-U74BV2:113769_AT mannosidase, alpha, class 1A, member 2 mm
MG-U74BV2:110305_AT mannosidase, alpha, class 1A, member 2 mm
MOE430A:1420976_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430A_2:1420976_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430_2:1420976_AT mannosidase, alpha, class 1A, member 2 mm
MOE430A:1420977_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430A_2:1420977_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430_2:1420977_AT mannosidase, alpha, class 1A, member 2 mm
MOE430B:1434395_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430_2:1434395_AT mannosidase, alpha, class 1A, member 2 mm
MOUSE430_2:1446565_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOE430B:1446565_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOE430B:1440259_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOUSE430_2:1440259_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOUSE430A_2:1456534_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOE430A:1456534_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MOUSE430_2:1456534_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
MU19KSUBC:TC36254_AT Mannosidase, alpha, class 1A, member 2 (Man1a2), mRNA mm
RAE230A:1375261_AT Mannosidase 1, beta (predicted) rn
RAT230_2:1375261_AT Mannosidase 1, beta (predicted) rn
RG-U34B:RC_AI011360_AT Mannosidase 1, beta (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
18346 protein amino acid prenylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
8318 protein prenyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01415 Hypothetical protein C52E4.5 [Caenorhabditis elegans] ref|NP_506006.1| C52E4.5 [Caenorhabditis elegans] 0.0
blast CAE75363 Hypothetical protein CBG23347 [Caenorhabditis briggsae] 0.0
blast EAA06297 ENSANGP00000017297 [Anopheles gambiae str. PEST] ref|XP_310525.2| ENSANGP00000017297 [Anopheles gambiae str. PEST] 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR001382 EMBL-EBI Glycoside hydrolase, family 47 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506006.1 1 12-31
C52E4.5 1 12-31
SNAP00000019787 1 12-31
GENEFINDER00000019794 1 12-31
Sequence
Target Sequence 
>C. ELEGANS:190323_S_AT
gggacgtcagtgatgctattcagaaacacatgattaaagtttccaaacaatcaaatctta
catacacagttgagttgaacaatggtcaagctcaacataaaatgggacatctcgcttgtt
ttgtacctggaatgtttgctctacaagccatcaacgaggatactgaagaagagaaattaa
gaattatgacattggcggaagagttggcgaaaacctgccatgaaagctatattcgttccg
agacccacattggacctgaaatgttctacttcaatgaacgtgacgaagcaaccagtaaac
actcggaaaatggatatattcaaagaccagaagtcattgaaggatggttctatttatgga
gattaactggaaaaacaatgtatagagattgggtatgggatgctgttcaagctattgaga
agtactgtagagttgatagtggatttactggtctacaaaatgtttataatcctaaagctg
gccgtgaggacgtgatgcaatccttcttccttgccgagtttctgaagtatgcctatttga
cgtttgcagacgaatcactgatttcactcgataaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGACGTCAGTGATGCTATTCAGAA 278 495 1163 Antisense
AAATGGGACATCTCGCTTGTTTTGT 266 113 1262 Antisense
ATCTCGCTTGTTTTGTACCTGGAAT 348 33 1271 Antisense
GAATGTTTGCTCTACAAGCCATCAA 415 331 1292 Antisense
GAAAGCTATATTCGTTCCGAGACCC 30 361 1384 Antisense
TTCCGAGACCCACATTGGACCTGAA 43 699 1398 Antisense
GAGATTGGGTATGGGATGCTGTTCA 473 395 1547 Antisense
AGGACGTGATGCAATCCTTCTTCCT 481 55 1649 Antisense
CTTCTTCCTTGCCGAGTTTCTGAAG 311 221 1665 Antisense
GTATGCCTATTTGACGTTTGCAGAC 258 455 1689 Antisense
CGAATCACTGATTTCACTCGATAAA 330 253 1713 Antisense
Sequence Source Affymetrix Proprietary Database