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[190322_at] disulphide bond domain

Description E03A3.5 /REP_DB=WormBase Gene ID /WP=CE25839 /TR=Q19044 /GB=CAA86230.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=disulphide bond domain
Cluster 6grp_total > 21      4grp_total > 42      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 4.8
(-3)
A 		5.4
(-2.8)
A 		22.2
(3)
A 		12.1
(-0.5)
A 		17
B 22.2
(-2.5)
A 		43.1
(3)
A 		38.4
(1.8)
A 		20.4
(-3)
A 		23
C 23.4
(-1)
A 		58.7
(3)
A 		5.1
(-3)
A 		27.9
(-0.4)
A 		54
avr 16.8
(-3) 		35.7
(3) 		21.9
(-1.4) 		20.1
(-2) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 6.9
(-1.8)
A 		26.5
(2.3)
A 		30.1
(3)
A 		5
(-2.2)
A 		21.7
(1.3)
A 		1.2
(-3)
A 		29
B 3.9
(-2.3)
A 		13.9
(0.7)
A 		21.6
(3)
A 		1.7
(-3)
A 		1.9
(-2.9)
A 		2.7
(-2.7)
A 		20
C 12.6
(0.2)
A 		23
(3)
A 		5.7
(-1.7)
A 		3.5
(-2.3)
A 		1.1
(-3)
A 		3.8
(-2.3)
A 		22
avr 7.8
(-1.3) 		21.1
(3) 		19.1
(2.4) 		3.4
(-2.7) 		8.2
(-1.2) 		2.6
(-3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 1.8
(-2.2)
A 		1.2
(-3)
A 		5.8
(3)
A 		1.8
(-2.2)
A 		5
B 7.1
(-0.3)
A 		10.6
(3)
A 		8.9
(1.4)
A 		4.3
(-3)
A 		6
C 7.8
(-0.6)
A 		16.9
(3)
A 		1.8
(-3)
A 		4.7
(-1.8)
A 		15
avr 5.6
(-1) 		9.6
(3) 		5.5
(-1.1) 		3.6
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1.3
(-2.3)
A 		9.4
(3)
A 		6.6
(1.2)
A 		1.3
(-2.3)
A 		6.6
(1.2)
A 		0.3
(-3)
A 		9
B 1.4
(-1.9)
A 		4.6
(2.2)
A 		5.2
(3)
A 		0.5
(-3)
A 		0.8
(-2.6)
A 		1
(-2.4)
A 		5
C 4.7
(0.9)
A 		7
(3)
A 		1.5
(-2)
A 		0.7
(-2.7)
A 		0.4
(-3)
A 		1.3
(-2.2)
A 		7
avr 2.5
(-1.4) 		7
(3) 		4.4
(0.5) 		0.8
(-3) 		2.6
(-1.3) 		0.9
(-2.9) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190322_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8087
Sequence Type Exemplar sequence
Representative Public ID E03A3.5 NCBI
Target Description E03A3.5 /REP_DB=WormBase Gene ID /WP=CE25839 /TR=Q19044 /GB=CAA86230.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=disulphide bond domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065412(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065412 NCBI Caenorhabditis elegans E03A3.5 (E03A3.5) mRNA, complete cds. 11/11 None
GENEFINDER00000016647 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4061171:4064028:1 9/11 None
SNAP00000016642 ENSEMBL cdna:SNAP chromosome:CEL140:III:4062206:4062793:1 9/11 None
E03A3.5 ENSEMBL cdna:known chromosome:CEL140:III:4060562:4062793:1 gene:E03A3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4060561-4062793(+) 100.0 100.0
Public Domain and Genome References
Gene Title disulphide bond domain
Ensembl E03A3.5
Entrez Gene 184007 Entrez gene
SwissProt Q19044 EMBL-EBI
WormBase CE25839 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86230 Hypothetical protein E03A3.5 [Caenorhabditis elegans] ref|NP_497813.1| E03A3.5 [Caenorhabditis elegans] 0.0
blast CAE60894 Hypothetical protein CBG04609 [Caenorhabditis briggsae] 1.0E-142
blast CAA86230 Hypothetical protein E03A3.5 [Caenorhabditis elegans] ref|NP_497813.1| E03A3.5 [Caenorhabditis elegans] 1.0E-132
blast CAE60894 Hypothetical protein CBG04609 [Caenorhabditis briggsae] 1.0E-105
blast CAA86230 Hypothetical protein E03A3.5 [Caenorhabditis elegans] ref|NP_497813.1| E03A3.5 [Caenorhabditis elegans] 8.0E-89
blast CAE60889 Hypothetical protein CBG04604 [Caenorhabditis briggsae] 4.0E-68
blast CAE60894 Hypothetical protein CBG04609 [Caenorhabditis briggsae] 7.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR000859 EMBL-EBI CUB 2.1E-7
Pfam IPR000859 EMBL-EBI CUB 1.8E-10
Pfam IPR000859 EMBL-EBI CUB 2.1E-7
Pfam IPR000859 EMBL-EBI CUB 4.9E-7
Pfam IPR000859 EMBL-EBI CUB 1.8E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000016647 1 362-379
Sequence
Target Sequence 
>C. ELEGANS:190322_AT
gcttgtattatatttatccacccgagggattctcagtgaatatttatttttcaaaaatca
aaacggaaaaaaattatgatttcgtggaaatttacgagaagaacagcacattgtatcctg
atcgaattgttggactatctggtaacatcagtgattacacatatcgttcaaacggaagct
tccttctaattgtcttccgtacaaactatgtaatcactgatactggattttatgcatgga
atgttgaacgagttcagcctccaattatatcaaatacgactacaagtggagaactaacat
caccgaattacccaaataattatgacacatctgacaaacagctttactacattcaagttg
ctcaaggagcacagatcaacgtgaccatcactgatttcctgactcaacagacttttgatt
atttggaaatattcgatagctttaatcaaacaattacaaagcctgtatcaagactctccg
gtaatttagttgttgctccatggaactggctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTGTATTATATTTATCCACCCGA 412 307 407 Antisense
TCCACCCGAGGGATTCTCAGTGAAT 630 587 423 Antisense
GAACAGCACATTGTATCCTGATCGA 263 351 507 Antisense
GAAGCTTCCTTCTAATTGTCTTCCG 626 341 581 Antisense
GAACGAGTTCAGCCTCCAATTATAT 502 347 652 Antisense
GAACTAACATCACCGAATTACCCAA 593 347 697 Antisense
GAGCACAGATCAACGTGACCATCAC 633 389 773 Antisense
TGACCATCACTGATTTCCTGACTCA 710 569 788 Antisense
ACAAAGCCTGTATCAAGACTCTCCG 531 111 862 Antisense
GACTCTCCGGTAATTTAGTTGTTGC 454 371 878 Antisense
GTTGTTGCTCCATGGAACTGGCTAA 260 437 895 Antisense
Sequence Source Affymetrix Proprietary Database