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[190318_at] alcohol dehydrogenase

Description R05D8.9 /REP_DB=WormBase Gene ID /WP=CE21093 /TR=Q9N5G2 /GB=AAF39944.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=alcohol dehydrogenase
Cluster 6grp_total > 63      4grp_total > 41      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 5.5
(-3)
A 		28
(3)
A 		10.2
(-1.7)
A 		11.1
(-1.5)
A 		23
B 3.2
(-3)
A 		3.6
(-2.8)
A 		16.1
(3)
A 		4.2
(-2.5)
A 		13
C 6.5
(0.8)
A 		4.2
(-3)
A 		7.1
(1.8)
A 		7.8
(3)
A 		4
avr 5.1
(-3) 		11.9
(3) 		11.1
(2.3) 		7.7
(-0.7) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 53
(-0.4)
A 		6.3
(-3)
A 		116
(3)
M 		72.3
(0.6)
A 		20.3
(-2.2)
A 		7.1
(-3)
A 		110
B 37.2
(-0.3)
A 		8.2
(-3)
A 		73.6
(3)
A 		46
(0.5)
A 		18.2
(-2.1)
A 		57.4
(1.5)
A 		65
C 23.8
(-3)
A 		28.8
(-2.6)
A 		103.9
(3)
P 		48.5
(-1.1)
A 		40.1
(-1.8)
A 		60
(-0.3)
A 		80
avr 38
(-1.3) 		14.4
(-3) 		97.8
(3) 		55.6
(-0) 		26.2
(-2.2) 		41.5
(-1.1) 		83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 2
(-2.5)
A 		6.1
(3)
A 		2.7
(-1.5)
A 		1.6
(-3)
A 		5
B 1
(-2.8)
A 		0.9
(-3)
A 		3.7
(3)
A 		0.9
(-3)
A 		3
C 2.2
(1.6)
A 		1.2
(-3)
A 		2.5
(3)
A 		1.3
(-2.5)
A 		1
avr 1.7
(-1.6) 		2.7
(1.9) 		3
(3) 		1.3
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 9.8
(-0.9)
A 		2.3
(-2.8)
A 		25.3
(3)
M 		18.3
(1.2)
A 		6.1
(-1.9)
A 		1.7
(-3)
A 		24
B 12.9
(0.3)
A 		2.7
(-3)
A 		17.9
(1.9)
A 		12.2
(0)
A 		7.4
(-1.5)
A 		21.4
(3)
A 		19
C 8.8
(-3)
A 		8.8
(-3)
A 		26.5
(3)
P 		9.8
(-2.7)
A 		13
(-1.6)
A 		20.1
(0.8)
A 		18
avr 10.5
(-1.1) 		4.6
(-3) 		23.2
(3) 		13.4
(-0.2) 		8.8
(-1.6) 		14.4
(0.2) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190318_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15660
Sequence Type Exemplar sequence
Representative Public ID R05D8.9 NCBI
Target Description R05D8.9 /REP_DB=WormBase Gene ID /WP=CE21093 /TR=Q9N5G2 /GB=AAF39944.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=alcohol dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071352(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071352 NCBI Caenorhabditis elegans R05D8.9 (R05D8.9) mRNA, complete cds. 11/11 None
SNAP00000009123 ENSEMBL cdna:SNAP chromosome:CEL140:V:2766098:2769580:-1 11/11 None
GENEFINDER00000009144 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2763846:2767330:-1 9/11 None
R05D8.9 ENSEMBL cdna:known chromosome:CEL140:V:2766098:2767330:-1 gene:R05D8.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2766097-2767330(-) 100.0 100.0
Public Domain and Genome References
Gene Title alcohol dehydrogenase
Ensembl R05D8.9
Entrez Gene 187609 Entrez gene
SwissProt Q9N5G2 EMBL-EBI
WormBase CE21093 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257775_AT short-chain dehydrogenase/reductase (SDR) family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE62263 Hypothetical protein CBG06322 [Caenorhabditis briggsae] 0.0
blast AAK18930 Hypothetical protein F12E12.7 [Caenorhabditis elegans] ref|NP_494640.1| F12E12.7 [Caenorhabditis elegans] 1.0E-144
blast NP_503753 R05D8.9 [Caenorhabditis elegans] gb|AAF39944.1| Hypothetical protein R05D8.9 [Caenorhabditis elegans] 1.0E-143
blast NP_503752 DeHydrogenases, Short chain family member (dhs-14) [Caenorhabditis elegans] gb|AAF39953.1| Dehydrogenases, short chain protein 14 [Caenorhabditis elegans] 1.0E-143
blast NP_503754 DeHydrogenases, Short chain family member (dhs-15) [Caenorhabditis elegans] gb|AAF39943.1| Dehydrogenases, short chain protein 15 [Caenorhabditis elegans] 1.0E-141
blast NP_503753 R05D8.9 [Caenorhabditis elegans] gb|AAF39944.1| Hypothetical protein R05D8.9 [Caenorhabditis elegans] 1.0E-135
blast CAE62263 Hypothetical protein CBG06322 [Caenorhabditis briggsae] 1.0E-135
blast NP_503752 DeHydrogenases, Short chain family member (dhs-14) [Caenorhabditis elegans] gb|AAF39953.1| Dehydrogenases, short chain protein 14 [Caenorhabditis elegans] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 2.4E-56
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.0E-59
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 5.7E-50
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 7.9E-51
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.0E-60
Sequence
Target Sequence 
>C. ELEGANS:190318_AT
atgccggagcagctttcaacgacgatcaaggacgtgttggagttgatcaagatgtctcag
tttacgataaaattatgcagattaacatgagaagtgtggttactttgactcaaaaagcca
aggagcatttggttaaggctaagggagaaattgtcaatgtctcgagtatagctggaacag
ctcatgcgcaacccggagttatgtactacgcaatgtcaaagtcggctctcgatcaattca
ccaggtgtgcagccatcgatcttattcagtacggggtacgcgtgaactcggtgagcccgg
gaggtgtcactaccgggtttggagaagctatgggtatgccgagtggggcgtttgaggaga
tgatgaagtttatggagtcccgcaaggagtgcatcccttctggagctgtagctaaaccaa
tcgatattgccaatattattgcgttcttggctgatcgtaagctttcttcctatattattg
ggcagtcgatagtagctgatggaggatctacactggtcttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCGGAGCAGCTTTCAACGACGA 598 41 293 Antisense
TAGCTGGAACAGCTCATGCGCAACC 358 649 461 Antisense
GCGCAACCCGGAGTTATGTACTACG 585 287 478 Antisense
AAAGTCGGCTCTCGATCAATTCACC 623 121 510 Antisense
ATTCACCAGGTGTGCAGCCATCGAT 332 9 528 Antisense
TACGGGGTACGCGTGAACTCGGTGA 702 633 562 Antisense
TGAGCCCGGGAGGTGTCACTACCGG 279 413 584 Antisense
ATGGAGTCCCGCAAGGAGTGCATCC 176 51 664 Antisense
CATCCCTTCTGGAGCTGTAGCTAAA 113 211 684 Antisense
TGGCTGATCGTAAGCTTTCTTCCTA 234 545 740 Antisense
GATGGAGGATCTACACTGGTCTTGG 543 407 790 Antisense
Sequence Source Affymetrix Proprietary Database