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[190315_at] ras protein

Description C27B7.8 /REP_DB=WormBase Gene ID /WP=CE03037 /GEN=rrp-1 /TR=Q18246 /GB=CAA90983.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ras protein
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 85      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 73      6grp_total > 76      4grp_total > 53      6grp_highest_sub > In      6grp_cgc > 57      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 519.2
(2.8)
P 		474.3
(-0.5)
P 		521.4
(3)
P 		440
(-3)
P 		81
B 529.4
(2.9)
P 		530.8
(3)
P 		455.6
(-3)
P 		455.1
(-3)
P 		76
C 541.5
(0.9)
P 		547
(3)
P 		544.8
(2.1)
P 		531.5
(-3)
P 		16
avr 530
(3) 		517.4
(1.6) 		507.3
(0.5) 		475.5
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 357
1 2 3 4 5 6 range
A 386.2
(-3)
P 		532.9
(-0.5)
P 		534.2
(-0.5)
P 		628.4
(1.1)
P 		536.3
(-0.5)
P 		742.8
(3)
P 		357
B 455.3
(-3)
P 		516.7
(-1.6)
P 		485.1
(-2.3)
P 		534.1
(-1.2)
P 		478.4
(-2.5)
P 		716.7
(3)
P 		261
C 528
(-1.5)
P 		477.9
(-3)
P 		683.7
(3)
P 		487.7
(-2.7)
P 		565.2
(-0.5)
P 		563.2
(-0.5)
P 		206
avr 456.5
(-3) 		509.2
(-1.5) 		567.7
(0.1) 		550.1
(-0.4) 		526.6
(-1.1) 		674.2
(3) 		218
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 189
(3)
P 		103
(-1.1)
P 		136.3
(0.5)
P 		64.4
(-3)
P 		125
B 168.8
(3)
P 		131.1
(-0.2)
P 		105.6
(-2.3)
P 		97
(-3)
P 		72
C 179.2
(2.4)
P 		157.3
(1)
P 		189.9
(3)
P 		89.6
(-3)
P 		100
avr 179
(3) 		130.5
(-0.1) 		143.9
(0.8) 		83.7
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 71.2
(-3)
P 		189.5
(3)
P 		116.3
(-0.7)
P 		159.4
(1.5)
P 		161.8
(1.6)
P 		180.8
(2.6)
P 		118
B 158.4
(-1.4)
P 		172
(-0.8)
P 		117.8
(-3)
P 		141.5
(-2)
P 		194.4
(0.1)
P 		267.3
(3)
P 		150
C 195.7
(3)
P 		145.8
(-0.1)
P 		174
(1.7)
P 		98.1
(-3)
P 		182.9
(2.2)
P 		188.5
(2.6)
P 		98
avr 141.8
(-2.3) 		169.1
(-0.3) 		136
(-2.8) 		133
(-3) 		179.7
(0.5) 		212.2
(3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190315_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9835
Sequence Type Exemplar sequence
Representative Public ID C27B7.8 NCBI
Target Description C27B7.8 /REP_DB=WormBase Gene ID /WP=CE03037 /GEN=rrp-1 /TR=Q18246 /GB=CAA90983.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ras protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069148 NCBI Caenorhabditis elegans RAP homolog (vertebrate Rap GTPase family) family member (rap-1) (rap-1) mRNA, complete cds. 11/11 None
SNAP00000019247 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8916867:8918756:-1 11/11 None
GENEFINDER00000019253 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8916326:8918650:-1 10/11 None
C27B7.8 ENSEMBL cdna:known chromosome:CEL140:IV:8916299:8918872:-1 gene:C27B7.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8916872-8918762(-) 100.0 100.0
Public Domain and Genome References
Gene Title ras protein
Gene Symbol rap-1
Ensembl C27B7.8
Entrez Gene 177709 Entrez gene
SwissProt Q18246 EMBL-EBI
WormBase CE03037 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.20872.1.S1_S_AT similar to Ras-related protein Rap-1A (Ras-related protein Krev-1) cfa
CANINE_2:CFAAFFX.21140.1.S1_AT similar to Ras-related protein Rap-1A (Ras-related protein Krev-1) cfa
DROSOPHILA_2:1632691_AT Roughened dm
DROSGENOME1:142363_AT Roughened dm
CHICKEN:GGA.4383.1.S1_AT similar to turkey RAP1Ab gga
HG-U95AV2:1848_AT RAP1A, member of RAS oncogene family hs
HC-G110:1848_AT RAP1A, member of RAS oncogene family hs
HU35KSUBD:RC_AA437308_S_AT RAP1A, member of RAS oncogene family hs
HUGENEFL:M22995_AT RAP1A, member of RAS oncogene family hs
HG-U133A:202362_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:202362_AT RAP1A, member of RAS oncogene family hs
HG-FOCUS:202362_AT RAP1A, member of RAS oncogene family hs
HG-U133A_2:202362_AT RAP1A, member of RAS oncogene family hs
U133_X3P:G4506412_3P_AT RAP1A, member of RAS oncogene family hs
U133_X3P:HS2AFFX.1.213.S1_3P_X_AT RAP1A, member of RAS oncogene family hs
U133_X3P:HS2AFFX.1.213.S1_3P_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:1555339_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:1555340_X_AT RAP1A, member of RAS oncogene family hs
HG-U95AV2:38756_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:228548_AT RAP1A, member of RAS oncogene family hs
HG-U133B:228548_AT RAP1A, member of RAS oncogene family hs
HU35KSUBB:RC_AA021557_AT RAP1A, member of RAS oncogene family hs
HUGENEFL:U48697_AT RAP1A, member of RAS oncogene family hs
U133_X3P:HS.306647.0.S1_3P_AT RAP1A, member of RAS oncogene family hs
HG-U95B:45178_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:239409_AT RAP1A, member of RAS oncogene family hs
HG-U133B:239409_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:240215_AT RAP1A, member of RAS oncogene family hs
HG-U133B:240215_AT RAP1A, member of RAS oncogene family hs
HG-U133_PLUS_2:243527_AT RAP1A, member of RAS oncogene family hs
HG-U133B:243527_AT RAP1A, member of RAS oncogene family hs
HG-U95C:53362_R_AT RAP1A, member of RAS oncogene family hs
HG-U95E:68257_AT RAP1A, member of RAS oncogene family hs
HG-U95D:68767_AT RAP1A, member of RAS oncogene family hs
HG-U95E:70281_AT RAP1A, member of RAS oncogene family hs
HG-U95E:83536_AT RAP1A, member of RAS oncogene family hs
HU35KSUBA:N76780_AT RAP1A, member of RAS oncogene family hs
U133_X3P:HS.155113.0.A1_3P_AT RAP1A, member of RAS oncogene family hs
U133_X3P:HS.175619.0.A1_3P_AT RAP1A, member of RAS oncogene family hs
MU11KSUBB:MSA.10101.0_F_AT RAS-related protein-1a mm
MG-U74AV2:100048_AT RAS-related protein-1a mm
MOE430A:1424139_AT RAS-related protein-1a mm
MOUSE430A_2:1424139_AT RAS-related protein-1a mm
MOUSE430_2:1424139_AT RAS-related protein-1a mm
MOUSE430_2:1448020_AT RAS-related protein-1a (Rap1a), mRNA mm
MOUSE430A_2:1448020_AT RAS-related protein-1a (Rap1a), mRNA mm
MOE430A:1448020_AT RAS-related protein-1a (Rap1a), mRNA mm
MG-U74AV2:93962_AT RAS-related protein-1a (Rap1a), mRNA mm
MU11KSUBA:AA210264_S_AT RAS-related protein-1a (Rap1a), mRNA mm
MU19KSUBA:TC15323_AT RAS-related protein-1a (Rap1a), mRNA mm
RG-U34B:RC_AA925664_AT Ras-related protein RAP-1A rn
RG-U34B:RC_AA943817_AT Ras-related protein RAP-1A rn
RAE230A:1390201_AT Ras-related protein RAP-1A rn
RAT230_2:1390201_AT Ras-related protein RAP-1A rn
RAE230A:1388615_AT Ras-related protein RAP-1A rn
RAT230_2:1388615_AT Ras-related protein RAP-1A rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
3924 GTPase activity inferred from sequence similarity QuickGO AmiGO
5515 protein binding inferred from physical interaction QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90983 Hypothetical protein C27B7.8 [Caenorhabditis elegans] ref|NP_501549.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-1) [Caenorhabditis elegans] emb|CAE74068.1| Hypothetical protein CBG21720 [Caenorhabditis briggsae] 1.0E-103
blast CAA90983 Hypothetical protein C27B7.8 [Caenorhabditis elegans] ref|NP_501549.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-1) [Caenorhabditis elegans] emb|CAE74068.1| Hypothetical protein CBG21720 [Caenorhabditis briggsae] 1.0E-91
blast XP_396692 PREDICTED: similar to ENSANGP00000020068 [Apis mellifera] 7.0E-86
blast XP_785316 PREDICTED: similar to RAS related protein 1b [Strongylocentrotus purpuratus] 1.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.1E-94
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.3E-80
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019253 1 206-225
Sequence
Target Sequence 
>C. ELEGANS:190315_AT
atccgcactgacggtacaattcgtgcagggaattttcgtagagaagtatgacccaacaat
tgaggatagttacagaaagcaagtggaagtcgacggccagcaatgtatgcttgagatact
ggatacagcaggaacggagcaattcacagcgatgcgtgatttgtacatgaagaatggaca
aggatttgttctcgtttattcaattactgctcaatcgacattcaatgatcttatggatct
tcgtgatcagattcttcgtgtcaaagatacggacgaggttccaatgattttggtaggaaa
taagtgtgatttggaagacgaacgagttgttggaaaagatcagggacagaatctcgccag
gcagtttggatcagcattccttgaaacctctgcaaaagcaaagattaacgtcagcgaggt
gttctacgacctcgttcgtcaaataaaccgacgttatcccgaatctggtcgacgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCGCACTGACGGTACAATTCGTG 601 37 60 Antisense
GAAGTCGACGGCCAGCAATGTATGC 113 337 145 Antisense
ACGGAGCAATTCACAGCGATGCGTG 498 95 193 Antisense
GTTCTCGTTTATTCAATTACTGCTC 27 443 247 Antisense
ATTACTGCTCAATCGACATTCAATG 402 13 262 Antisense
TCTTCGTGATCAGATTCTTCGTGTC 544 617 297 Antisense
ATCTCGCCAGGCAGTTTGGATCAGC 108 33 410 Antisense
GATCAGCATTCCTTGAAACCTCTGC 490 421 428 Antisense
AACGTCAGCGAGGTGTTCTACGACC 77 143 466 Antisense
GTTCTACGACCTCGTTCGTCAAATA 571 443 480 Antisense
ACGTTATCCCGAATCTGGTCGACGC 454 93 510 Antisense
Sequence Source Affymetrix Proprietary Database