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[190308_at]

Description F46B6.6 /REP_DB=WormBase Gene ID /WP=CE26974 /TR=Q20447 /GB=CAA94822.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=initiation factor IF-2
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 73      6grp_total > 65      4grp_total > 51      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 331
1 2 3 4 range
A 595.8
(2.3)
P 		499.7
(-3)
P 		514.1
(-2.2)
P 		609.4
(3)
P 		110
B 582.5
(-1)
P 		731.5
(3)
P 		566
(-1.4)
P 		508.3
(-3)
P 		223
C 619.5
(0.6)
P 		753.8
(3)
P 		696.8
(2)
P 		422.6
(-3)
P 		331
avr 599.3
(0.5) 		661.7
(3) 		592.3
(0.2) 		513.4
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 456.1
(-1.4)
P 		530.7
(0.1)
P 		536.8
(0.3)
P 		669.1
(3)
P 		380.1
(-3)
P 		664.9
(2.9)
P 		289
B 480.1
(-2.5)
P 		468.9
(-3)
P 		615.4
(3)
P 		577.1
(1.4)
P 		506.3
(-1.5)
P 		605.5
(2.6)
P 		147
C 634.5
(2.1)
P 		433.3
(-2.3)
P 		557.6
(0.4)
P 		653.4
(2.5)
P 		399.3
(-3)
P 		678.3
(3)
P 		279
avr 523.6
(-0.4) 		477.6
(-1.7) 		569.9
(0.8) 		633.2
(2.6) 		428.6
(-3) 		649.6
(3) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 216.9
(3)
P 		108.5
(-2.1)
P 		134.4
(-0.9)
P 		89.2
(-3)
P 		128
B 185.7
(3)
P 		180.7
(2.6)
P 		131.2
(-1.2)
P 		108.3
(-3)
P 		77
C 205.1
(1.7)
P 		216.7
(2.1)
P 		242.9
(3)
P 		71.3
(-3)
P 		172
avr 202.6
(3) 		168.6
(1.2) 		169.5
(1.2) 		89.6
(-3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 84.1
(-3)
P 		188.8
(3)
P 		116.9
(-1.1)
P 		169.7
(1.9)
P 		114.7
(-1.2)
P 		161.9
(1.5)
P 		105
B 167
(-1.6)
P 		156.1
(-2.5)
P 		149.4
(-3)
P 		152.9
(-2.7)
P 		205.7
(1.4)
P 		225.8
(3)
P 		76
C 235.2
(3)
P 		132.2
(-2.8)
P 		141.9
(-2.3)
P 		131.4
(-2.9)
P 		129.2
(-3)
P 		227.1
(2.5)
P 		106
avr 162.1
(-0.7) 		159
(-1) 		136.1
(-3) 		151.3
(-1.7) 		149.9
(-1.8) 		204.9
(3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190308_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11632
Sequence Type Exemplar sequence
Representative Public ID F46B6.6 NCBI
Target Description F46B6.6 /REP_DB=WormBase Gene ID /WP=CE26974 /TR=Q20447 /GB=CAA94822.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=initiation factor IF-2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NR_002401(11), NM_001028765(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NR_002401 NCBI Caenorhabditis elegans F46B6.6, miscRNA. 11/11 None
NM_001028765 NCBI Caenorhabditis elegans F46B6.6a (F46B6.6) mRNA, complete cds. 11/11 None
SNAP00000041198 ENSEMBL cdna:SNAP chromosome:CEL140:V:9785924:9789348:-1 11/11 None
GENEFINDER00000041213 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9785924:9789407:-1 11/11 None
F46B6.6 ENSEMBL cdna:known chromosome:CEL140:V:9785760:9789450:-1 gene:F46B6.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9785923-9788766(-) 99.53 99.53
Public Domain and Genome References
Ensembl F46B6.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94822 Hypothetical protein F46B6.6a [Caenorhabditis elegans] ref|NP_001023936.1| F46B6.6a [Caenorhabditis elegans] 0.0
blast CAE72254 Hypothetical protein CBG19373 [Caenorhabditis briggsae] 0.0
blast CAI36008 mitochondrial translational initiation factor 2 [Mus musculus] dbj|BAE22940.1| unnamed protein product [Mus musculus] dbj|BAE27568.1| unnamed protein product [Mus musculus] 1.0E-127
Protein Domains
Database ID Description E-Value
scop a.4.5.C-terminal fragment of elongation factor SelB All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; C-terminal fragment of elongation factor SelB 6.09999990463257
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 3.0E-10
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 3.9E-47
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 3.0E-10
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 3.9E-47
Sequence
Target Sequence 
>C. ELEGANS:190308_AT
gtagaaggcacactcgaggcgattctggaaattttgagcacatataaaagcgagcaatgc
aagctacaattggttgatttcgaagtgggaccgccgactgaaaaagacattgaattggca
aaagagacggatgccacaatatacacattcaacgtagaaacatctgcaaaactcaaacaa
cgtgccgatgctgctggtattcaaatcgattcattcaatgtcatttatcgcatgattgaa
tcgctgaaaggagaactttctgctcgtattccaaagctaactgaactccatttagttgga
gaaggacacgtgctcaaagaatttgtcatttcggatcgtggaagaaagcggcaacctatt
gcaggaacccttgtgaattggggaaactttgatagacattgcatttataagttcactcga
ggcaatcaagtcgtttacgaaggagaagtcgaaactatgaaagctggaatggaagtggtg
acgaatgcaaaaacgaacacagaagtcggtctcgcgttgtctgataaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGAAGGCACACTCGAGGCGATTC 474 455 1294 Antisense
TGATTTCGAAGTGGGACCGCCGACT 680 563 1368 Antisense
GACGGATGCCACAATATACACATTC 337 377 1419 Antisense
AACTCAAACAACGTGCCGATGCTGC 71 139 1463 Antisense
GTGCCGATGCTGCTGGTATTCAAAT 248 479 1475 Antisense
GAACTTTCTGCTCGTATTCCAAAGC 621 349 1546 Antisense
TCCAAAGCTAACTGAACTCCATTTA 513 585 1563 Antisense
GGCAACCTATTGCAGGAACCCTTGT 503 529 1643 Antisense
AGTTCACTCGAGGCAATCAAGTCGT 635 61 1703 Antisense
CGAACACAGAAGTCGGTCTCGCGTT 318 253 1787 Antisense
TCGGTCTCGCGTTGTCTGATAAAAA 609 605 1799 Antisense
Sequence Source Affymetrix Proprietary Database