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[190277_at] sulfate transporter

Description W01B11.2 /REP_DB=WormBase Gene ID /WP=CE18306 /TR=O45016 /GB=AAK21473.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=sulfate transporter
Cluster 6grp_total > 88      4grp_total > 35      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 7.8
(-2.2)
A 		20.4
(3)
A 		5.9
(-3)
A 		12.7
(-0.2)
A 		15
B 9.8
(-2.6)
A 		70.7
(3)
A 		6
(-3)
A 		10.5
(-2.6)
A 		65
C 8.4
(-2.7)
A 		10.7
(-2.3)
A 		6.7
(-3)
A 		39.7
(3)
A 		33
avr 8.7
(-2.5) 		33.9
(3) 		6.2
(-3) 		21
(0.2) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 10.9
(-3)
A 		11.4
(-2.9)
A 		12.2
(-2.8)
A 		17.1
(-2)
A 		24.8
(-0.7)
A 		46.9
(3)
A 		36
B 5.1
(-3)
A 		15.1
(-1.1)
A 		37.3
(3)
A 		10.4
(-2)
A 		31.3
(1.9)
A 		14.6
(-1.2)
A 		32
C 14
(-2.4)
A 		28.1
(-0.5)
A 		9.4
(-3)
A 		9.9
(-2.9)
A 		27.9
(-0.5)
A 		54.7
(3)
A 		45
avr 10
(-3) 		18.2
(-1.3) 		19.6
(-1) 		12.5
(-2.5) 		28
(0.8) 		38.7
(3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.8
(-0.4)
A 		4.4
(3)
A 		1.6
(-3)
A 		1.9
(-2.4)
A 		3
B 3.1
(-2.4)
A 		17.5
(3)
A 		1.4
(-3)
A 		2.2
(-2.7)
A 		16
C 2.8
(-2.3)
A 		3.1
(-1.9)
A 		2.3
(-3)
A 		6.7
(3)
A 		4
avr 2.9
(-2) 		8.3
(3) 		1.8
(-3) 		3.6
(-1.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2
(-3)
A 		4.1
(-1.7)
A 		2.7
(-2.6)
A 		4.3
(-1.5)
A 		7.5
(0.5)
A 		11.4
(3)
A 		9
B 1.8
(-3)
A 		5
(-1.2)
A 		9
(1)
A 		2.8
(-2.4)
A 		12.7
(3)
A 		5.5
(-1)
A 		11
C 5.2
(-1.8)
A 		8.6
(-0.6)
A 		2.4
(-2.9)
A 		2
(-3)
A 		9
(-0.4)
A 		18.3
(3)
A 		16
avr 3
(-3) 		5.9
(-1) 		4.7
(-1.8) 		3
(-3) 		9.7
(1.6) 		11.7
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190277_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.46
Sequence Type Exemplar sequence
Representative Public ID W01B11.2 NCBI
Target Description W01B11.2 /REP_DB=WormBase Gene ID /WP=CE18306 /TR=O45016 /GB=AAK21473.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=sulfate transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058737(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058737 NCBI Caenorhabditis elegans SULfate Permease family member (sulp-6) (sulp-6) mRNA, complete cds. 11/11 None
SNAP00000027450 ENSEMBL cdna:SNAP chromosome:CEL140:I:3287381:3293128:1 11/11 None
GENEFINDER00000027457 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3287381:3293128:1 11/11 None
W01B11.2 ENSEMBL cdna:known chromosome:CEL140:I:3287480:3293128:1 gene:W01B11.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3287479-3293128(+) 96.17 100.0
Public Domain and Genome References
Gene Title sulfate transporter
Gene Symbol sulp-6
Ensembl W01B11.2
Entrez Gene 189085 Entrez gene
SwissProt O45016 EMBL-EBI
WormBase CE18306 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491138 SULfate Permease family member (sulp-6) [Caenorhabditis elegans] gb|AAK21473.1| Sulfate permease family protein 6 [Caenorhabditis elegans] 0.0
blast CAE68988 Hypothetical protein CBG14974 [Caenorhabditis briggsae] 0.0
blast CAE68988 Hypothetical protein CBG14974 [Caenorhabditis briggsae] 1.0E-164
blast AAX34424 anion transporter SULP-6 [Caenorhabditis elegans] 9.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001902 EMBL-EBI Sulphate transporter 9.3E-25
Pfam IPR002645 EMBL-EBI Sulfate transporter/antisigma-factor antagonist STAS 2.5E-37
Pfam IPR001902 EMBL-EBI Sulphate transporter 7.3E-39
Pfam IPR002645 EMBL-EBI Sulfate transporter/antisigma-factor antagonist STAS 2.5E-37
Pfam IPR001902 EMBL-EBI Sulphate transporter 4.6E-50
Pfam IPR002645 EMBL-EBI Sulfate transporter/antisigma-factor antagonist STAS 2.5E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491138.1 5 94-116,121-143,207-229,281-303,361-383
W01B11.2 5 94-116,121-143,207-229,281-303,361-383
SNAP00000027450 10 127-149,153-175,182-201,232-254,336-358,417-439,456-478,493-514,521-543,553-575
GENEFINDER00000027457 10 127-149,153-175,182-201,232-254,314-336,395-417,434-456,471-492,499-521,531-553
Sequence
Target Sequence 
>C. ELEGANS:190277_AT
ccaatcccgccgatcagtatgagaggctcacccatattatcattgattgttctacaatta
tatatgtggatttgatgggtcaaggcgcgctgaaagatgtctacaacgactacaagacaa
tcggcatttcggttctttttgcaaatactaatgaacacgttcgccagaactttgaaactt
cgcagttcttcgaagaagtaccccggggtcgaatgtttgtcagtgtttccgatgcggttg
accaggcggagctcgagcaacgacacaagaccgagacagttcagacaacggcagctccga
aagcaacagtagttccaccagtggccaagtccgtcggccagaagtctgttccgattactg
aacctgtactcgtccccatacaaagttcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAATCCCGCCGATCAGTATGAGAG 525 277 1910 Antisense
GAGAGGCTCACCCATATTATCATTG 426 395 1930 Antisense
AGACAATCGGCATTTCGGTTCTTTT 691 71 2024 Antisense
TACCCCGGGGTCGAATGTTTGTCAG 298 567 2108 Antisense
ATGTTTGTCAGTGTTTCCGATGCGG 687 45 2122 Antisense
GTTGACCAGGCGGAGCTCGAGCAAC 651 437 2146 Antisense
AGACAGTTCAGACAACGGCAGCTCC 457 71 2183 Antisense
TAGTTCCACCAGTGGCCAAGTCCGT 614 613 2219 Antisense
GGCCAGAAGTCTGTTCCGATTACTG 349 541 2245 Antisense
CGATTACTGAACCTGTACTCGTCCC 169 249 2261 Antisense
TACTCGTCCCCATACAAAGTTCAGC 142 647 2276 Antisense
Sequence Source Affymetrix Proprietary Database