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[190258_at] alpha-mannosidase II

Description F48C1.1 /REP_DB=WormBase Gene ID /WP=CE10732 /TR=O01574 /GB=AAB52345.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=alpha-mannosidase II
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 55      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 90      6grp_total > 47      4grp_total > 33      6grp_highest_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 159.4
(1.4)
P
125.1
(-3)
P
136.7
(-1.5)
P
171.5
(3)
P
46
B 139.3
(-2.4)
P
185
(3)
P
134.5
(-3)
P
134.1
(-3)
P
51
C 85.5
(-3)
P
124.2
(0.6)
P
150.8
(3)
P
140.9
(2.1)
P
65
avr 128.1
(-3)
144.8
(1.8)
140.7
(0.7)
148.8
(3)
21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 164.7
(0.8)
P
106
(-2.3)
P
204.9
(3)
P
164.2
(0.8)
P
93.3
(-3)
P
156.7
(0.4)
P
112
B 150.4
(3)
P
135.9
(1)
P
106
(-3)
P
119.4
(-1.2)
P
133.4
(0.7)
P
149.6
(2.9)
P
44
C 124.2
(-0.7)
P
164.5
(3)
P
99.7
(-3)
P
146.1
(1.3)
P
111.6
(-1.9)
P
149.2
(1.6)
P
65
avr 146.4
(2.2)
135.5
(0.5)
136.9
(0.7)
143.2
(1.7)
112.8
(-3)
151.8
(3)
39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 58
(3)
P
27.2
(-2.6)
P
35.7
(-1.1)
P
25.1
(-3)
P
33
B 44.4
(2.5)
P
45.7
(3)
P
31.2
(-2.1)
P
28.6
(-3)
P
17
C 28.3
(-2.1)
P
35.7
(-0.5)
P
52.6
(3)
P
23.8
(-3)
P
29
avr 43.6
(3)
36.2
(0.5)
39.8
(1.7)
25.8
(-3)
18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 30.4
(-2.2)
P
37.7
(0.5)
P
44.6
(3)
P
41.6
(1.9)
P
28.1
(-3)
P
38.2
(0.7)
P
17
B 52.3
(2.3)
P
45.2
(0.9)
P
25.8
(-3)
P
31.6
(-1.8)
P
54.2
(2.7)
P
55.8
(3)
P
30
C 46
(2)
P
50.2
(3)
P
25.4
(-3)
P
29.4
(-2)
P
36.1
(-0.4)
P
49.9
(2.9)
P
25
avr 42.9
(1.1)
44.4
(1.7)
31.9
(-3)
34.2
(-2.1)
39.5
(-0.2)
48
(3)
16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190258_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.757
Sequence Type Exemplar sequence
Representative Public ID F48C1.1 NCBI
Target Description F48C1.1 /REP_DB=WormBase Gene ID /WP=CE10732 /TR=O01574 /GB=AAB52345.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=alpha-mannosidase II
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026303(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_001026303 NCBI Caenorhabditis elegans F48C1.1 (F48C1.1) mRNA, complete cds. 11/11 None
SNAP00000025985 ENSEMBL cdna:SNAP chromosome:CEL140:I:5325569:5330103:1 11/11 None
GENEFINDER00000025996 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5326616:5330103:1 11/11 None
F48C1.1a ENSEMBL cdna:known chromosome:CEL140:I:5324514:5330215:1 gene:F48C1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5324513-5330103(+) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-mannosidase II
Ensembl F48C1.1
Entrez Gene 172178 Entrez gene
SwissProt O01574 EMBL-EBI
WormBase CE36934 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4559 alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52345 Hypothetical protein F48C1.1 [Caenorhabditis elegans] ref|NP_001021474.1| F48C1.1 [Caenorhabditis elegans] 0.0
blast CAE66941 Hypothetical protein CBG12333 [Caenorhabditis briggsae] 0.0
blast XP_605840 PREDICTED: similar to mannosidase, alpha, class 2A, member 2 isoform 1 [Bos taurus] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.6E-16
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 9.6E-23
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021474.1 1 30-52
F48C1.1a 1 30-52
SNAP00000025985 1 12-34
Sequence
Target Sequence
>C. ELEGANS:190258_AT
gtggagcatggagcaagattcttggaaagcggaactgttgaaattaatattgatcgaatt
ttgaatcaagatgatggaaaaggtcttggaactggtccggatgcaattccaattgacatg
aaaccagttgatatgaaatttaaattaattttcgatagccttgaatcggatccaactgag
aattcccgttattcaacacacagtttccgtgctcaacaagcagttcaaacaatcatttat
ccaccgatgatgttcaataggccaccgaaaaaagaagaggatattgaagaaatcgaggaa
atttcgaatttgaaatttccgtgtgatattcaactattgactgtgagacctttggaagat
aataaacaattattgattttatatcgacatgcaaccgtgtgctctagtcaaaaagttgga
aactgtggtggtgaattgaagagttctctcatggatcttctcatcaaactgggtgcaa


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GTGGAGCATGGAGCAAGATTCTTGG 138 491 2338 Antisense
AGGTCTTGGAACTGGTCCGGATGCA 84 57 2418 Antisense
GATAGCCTTGAATCGGATCCAACTG 37 423 2491 Antisense
GGATCCAACTGAGAATTCCCGTTAT 642 483 2505 Antisense
AATTCCCGTTATTCAACACACAGTT 697 177 2518 Antisense
ACACAGTTTCCGTGCTCAACAAGCA 11 111 2535 Antisense
CCGATGATGTTCAATAGGCCACCGA 166 265 2581 Antisense
AACTATTGACTGTGAGACCTTTGGA 173 139 2669 Antisense
GACATGCAACCGTGTGCTCTAGTCA 402 369 2723 Antisense
TCTCTCATGGATCTTCTCATCAAAC 220 613 2782 Antisense
GATCTTCTCATCAAACTGGGTGCAA 463 419 2791 Antisense
Sequence Source Affymetrix Proprietary Database