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[190257_at]

Description F28C12.3 /REP_DB=WormBase Gene ID /WP=CE09750 /TR=O17843 /GB=CAB07599.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=7tm receptor protein
Cluster 6grp_total > 81      4grp_total > 36      6grp_cgc > 84      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 21.7
(-1.7)
A 		56.3
(3)
A 		11.8
(-3)
A 		18
(-2.2)
A 		45
B 44.7
(3)
A 		24.4
(-3)
A 		32.2
(-0.7)
A 		36.7
(0.6)
A 		20
C 19.7
(-1.3)
A 		23.3
(-0.1)
A 		15.1
(-3)
A 		31.8
(3)
A 		17
avr 28.7
(0.6) 		34.7
(3) 		19.7
(-3) 		28.8
(0.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 47.7
(2.2)
A 		7.9
(-2.4)
A 		43.2
(1.7)
P 		41.8
(1.6)
A 		54.3
(3)
A 		2.5
(-3)
A 		52
B 34.3
(-1.4)
P 		33.5
(-1.6)
A 		47
(0.9)
P 		58.1
(3)
A 		48.7
(1.2)
M 		25.9
(-3)
P 		32
C 25.1
(-3)
A 		56.1
(3)
P 		36.4
(-0.8)
A 		40.4
(-0)
A 		40.4
(-0)
P 		34.9
(-1.1)
P 		31
avr 35.7
(0.3) 		32.5
(-0.4) 		42.2
(1.7) 		46.8
(2.8) 		47.8
(3) 		21.1
(-3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 7.9
(0.3)
A 		12.2
(3)
A 		3.1
(-2.7)
A 		2.6
(-3)
A 		10
B 14.3
(3)
A 		6
(-3)
A 		7.5
(-1.9)
A 		7.8
(-1.7)
A 		8
C 6.5
(2.2)
A 		6.7
(3)
A 		5.2
(-3)
A 		5.4
(-2.2)
A 		2
avr 9.6
(3) 		8.3
(1.2) 		5.3
(-3) 		5.3
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 8.8
(0.1)
A 		2.8
(-2.2)
A 		9.4
(0.3)
P 		10.6
(0.8)
A 		16.4
(3)
A 		0.6
(-3)
A 		16
B 11.9
(-1.7)
P 		11.1
(-2.2)
A 		11.4
(-2)
P 		15.4
(0.4)
A 		19.8
(3)
M 		9.7
(-3)
P 		10
C 9.3
(-2.2)
A 		17.1
(3)
P 		9.3
(-2.2)
A 		8.1
(-3)
A 		13.1
(0.3)
P 		11.7
(-0.6)
P 		9
avr 10
(-1.2) 		10.3
(-1) 		10
(-1.2) 		11.4
(-0.3) 		16.4
(3) 		7.3
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190257_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.910
Sequence Type Exemplar sequence
Representative Public ID F28C12.3 NCBI
Target Description F28C12.3 /REP_DB=WormBase Gene ID /WP=CE09750 /TR=O17843 /GB=CAB07599.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=7tm receptor protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description Only library file information is available for this probe set. See probe design information above.
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000017844 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12702470:12706335:1 11/11 None
F28C12.3 ENSEMBL cdna:pseudogene chromosome:CEL140:I:12702527:12704055:1 gene:F28C12.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017841 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12702457-12703986(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28C12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07600 Hypothetical protein F28C12.4 [Caenorhabditis elegans] ref|NP_493216.1| Serpentine Receptor, class A (alpha) family member (sra-20) [Caenorhabditis elegans] sp|O17844|SRA20_CAEEL Serpentine receptor class alpha-20 (Protein sra-20) 1.0E-175
blast T21475 hypothetical protein F28C12.3 - Caenorhabditis elegans 1.0E-145
blast CAB04702 Hypothetical protein T06G6.2 [Caenorhabditis elegans] ref|NP_493220.2| Serpentine Receptor, class A (alpha) family member (sra-24) [Caenorhabditis elegans] sp|O62368|SRA24_CAEEL Serpentine receptor class alpha-24 (Protein sra-24) 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR000344 EMBL-EBI Nematode chemoreceptor, Sra 1.1E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000017844 10 121-143,170-192,218-240,255-277,331-353,412-429,442-464,491-508,536-558,573-595
Sequence
Target Sequence 
>C. ELEGANS:190257_AT
aatactttatcgctatatctctcctagtttttcagctttttcttacattattatcgtttt
acattgcattctacggggttcctttagcaggttacataccaatgtgcacatatcaccctg
cattatctgtttactatagtacgattaacaatgtgcgtactggagttatggtttcttgta
ttattgttaccatatttgtgtattacttaagtgtgaatttagagaagcgaatccaaaaaa
ccagttattcccctggagaacgatattctgcatacgaaaacgttacaacatccaaatcag
tatgcattttaattgttcttcaattctcatgcattatgatatcgtcatttggagtgaatt
tgcttatgatgagtgaaaaatcaatgtcagagaaagtgttccacacaattgttccgtttc
taccgggtgtaacctatgcaaatttatgtcttcctcttgtcatctttatccagacaacac
taacaattcggacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACTTTATCGCTATATCTCTCCT 162 173 311 Antisense
ATATCTCTCCTAGTTTTTCAGCTTT 149 21 325 Antisense
ATTATCGTTTTACATTGCATTCTAC 661 13 360 Antisense
TGCATTCTACGGGGTTCCTTTAGCA 442 569 375 Antisense
TACCAATGTGCACATATCACCCTGC 85 651 407 Antisense
ATCACCCTGCATTATCTGTTTACTA 274 29 422 Antisense
GTTCCACACAATTGTTCCGTTTCTA 238 441 708 Antisense
GTTTCTACCGGGTGTAACCTATGCA 431 447 726 Antisense
GCAAATTTATGTCTTCCTCTTGTCA 544 317 748 Antisense
TTCCTCTTGTCATCTTTATCCAGAC 69 701 761 Antisense
CAGACAACACTAACAATTCGGACTA 434 203 781 Antisense
Sequence Source Affymetrix Proprietary Database