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[190247_s_at]

Description Y25C1A.8 /REP_DB=WormBase Gene ID /WP=CE21476 /TR=Q9TYM0 /GB=AAD12844.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=zinc-finger protein
Cluster 6grp_total > 47      4grp_total > 13      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 101.1
(-0.7)
A 		152.3
(3)
A 		69.4
(-3)
A 		108.5
(-0.2)
A 		83
B 152.5
(3)
A 		76.5
(-3)
A 		99.3
(-1.2)
A 		127.8
(1.1)
A 		76
C 121.8
(-0.7)
A 		85.1
(-3)
A 		139.2
(0.4)
A 		179.9
(3)
A 		95
avr 125.1
(0.7) 		104.6
(-2.7) 		102.6
(-3) 		138.7
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 89
(-1.5)
A 		107.4
(-0.2)
A 		72
(-2.8)
A 		69.6
(-3)
A 		84.9
(-1.8)
A 		149.4
(3)
P 		80
B 84.8
(-0.1)
A 		115.1
(3)
A 		84.6
(-0.1)
A 		56.2
(-3)
A 		97.1
(1.2)
A 		111
(2.6)
P 		59
C 119.3
(1.7)
A 		126.4
(3)
A 		102.2
(-1.5)
A 		93.9
(-3)
A 		95.1
(-2.8)
P 		125.9
(2.9)
P 		33
avr 97.7
(-0.4) 		116.3
(1.7) 		86.3
(-1.6) 		73.2
(-3) 		92.4
(-0.9) 		128.8
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 36.8
(3)
A 		33.1
(1.9)
A 		18.1
(-2.4)
A 		15.9
(-3)
A 		21
B 48.6
(3)
A 		18.9
(-3)
A 		23
(-2.2)
A 		27.2
(-1.3)
A 		30
C 40.3
(0.9)
A 		24.5
(-3)
A 		48.5
(3)
A 		30.3
(-1.6)
A 		24
avr 41.9
(3) 		25.5
(-2.7) 		29.9
(-1.1) 		24.5
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 16.4
(-2.8)
A 		38.2
(3)
A 		15.7
(-3)
A 		17.7
(-2.5)
A 		25.6
(-0.4)
A 		36.4
(2.5)
P 		23
B 29.5
(0.3)
A 		38.3
(2.3)
A 		20.5
(-1.7)
A 		14.9
(-3)
A 		39.4
(2.5)
A 		41.4
(3)
P 		27
C 44.2
(3)
A 		38.6
(1.7)
A 		26
(-1.3)
A 		18.9
(-3)
A 		30.8
(-0.2)
P 		42.1
(2.5)
P 		25
avr 30
(0.4) 		38.4
(2.6) 		20.7
(-2.1) 		17.2
(-3) 		31.9
(0.9) 		40
(3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190247_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5203
Sequence Type Exemplar sequence
Representative Public ID Y25C1A.8 NCBI
Target Description Y25C1A.8 /REP_DB=WormBase Gene ID /WP=CE21476 /TR=Q9TYM0 /GB=AAD12844.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=zinc-finger protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182188(11), NM_062039(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182188 NCBI Caenorhabditis elegans Y25C1A.8b (Y25C1A.8) mRNA, complete cds. 11/11 None
NM_062039 NCBI Caenorhabditis elegans Y25C1A.8a (Y25C1A.8) mRNA, complete cds. 11/11 None
SNAP00000041877 ENSEMBL cdna:SNAP chromosome:CEL140:II:3058285:3076941:-1 10/11 None
Y25C1A.8b ENSEMBL cdna:known chromosome:CEL140:II:3071172:3076932:-1 gene:Y25C1A.8 11/11 None
Y25C1A.8a ENSEMBL cdna:known chromosome:CEL140:II:3071470:3076941:-1 gene:Y25C1A.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041896 6/11 Cross Hyb Matching Probes None
GENEFINDER00000041899 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3071468-3076940(-) 94.03 100.0
Public Domain and Genome References
Ensembl Y25C1A.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022420 Y25C1A.7c [Caenorhabditis elegans] gb|AAX88818.1| Hypothetical protein Y25C1A.7c [Caenorhabditis elegans] 1.0E-164
blast NP_494438 Y25C1A.7a [Caenorhabditis elegans] gb|AAX88817.1| Hypothetical protein Y25C1A.7a [Caenorhabditis elegans] 1.0E-132
blast NP_494439 Y25C1A.7b [Caenorhabditis elegans] gb|AAD12839.1| Hypothetical protein Y25C1A.7b [Caenorhabditis elegans] 1.0E-131
blast NP_494440 Y25C1A.8a [Caenorhabditis elegans] gb|AAD12844.1| Hypothetical protein Y25C1A.8a [Caenorhabditis elegans] 1.0E-121
blast CAE72461 Hypothetical protein CBG19634 [Caenorhabditis briggsae] 3.0E-86
blast NP_871988 Y25C1A.8b [Caenorhabditis elegans] gb|AAN60503.1| Hypothetical protein Y25C1A.8b [Caenorhabditis elegans] 1.0E-40
blast XP_392865 PREDICTED: similar to CG3732-PA [Apis mellifera] 5.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 3.0E-5
Pfam IPR006977 EMBL-EBI Protein of unknown function DUF649 4.8E-70
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000041877 4 448-470,491-510,520-542,547-569
Sequence
Target Sequence 
>C. ELEGANS:190247_S_AT
tgaaaaaacggactccacgtggagaagatgaagccgacgaagacgatgaagccgatgaag
atgaagatgaggatggagaagatcttggaaaatataatttcgattttgattccgatccgg
aattagtggaaaaagtcgagaatttgaagagcaaaattgtggcggtggcggcagctggct
cggaagcctgtgatgacagcgaatgctcatgctcatgctcgggcggtgaatgctcttgcc
cagaaagtgaagatgaaaaggaaaaagatcgtcgaaaaatgcacaaaacatcacgagaaa
gggatcgtgaaagccatcggaaacggagcaactcaccgagagatcgtgatagaaagagag
gaaaaagaagtcggagtagaagccgagaaagaagttatcgggatgatcggagagatcgca
agagatctcgttctcgcgaacgccgccgttccccgccacgtggcaccaccagtgatagac
gccgagacgacgagagacgcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAAAACGGACTCCACGTGGAGA 361 571 500 Antisense
AGAGCAAAATTGTGGCGGTGGCGGC 637 65 647 Antisense
GCAGCTGGCTCGGAAGCCTGTGATG 700 313 670 Antisense
CTCGGGCGGTGAATGCTCTTGCCCA 478 227 717 Antisense
GTGAATGCTCTTGCCCAGAAAGTGA 236 481 725 Antisense
GAAAGGGATCGTGAAAGCCATCGGA 234 359 796 Antisense
AGCCATCGGAAACGGAGCAACTCAC 43 83 811 Antisense
GGAGCAACTCACCGAGAGATCGTGA 706 519 824 Antisense
GCCACGTGGCACCACCAGTGATAGA 667 279 954 Antisense
GGCACCACCAGTGATAGACGCCGAG 84 535 961 Antisense
GACGCCGAGACGACGAGAGACGCAG 74 381 977 Antisense
Sequence Source Affymetrix Proprietary Database