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[190235_at] guanine nucleotide-binding protein

Description E02C12.5 /REP_DB=WormBase Gene ID /WP=CE04314 /GEN=gpa-3 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 15      4grp_total > 13      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 270
1 2 3 4 range
A 178.5
(-0.6)
P 		90.4
(-2.7)
P 		78.9
(-3)
A 		324.7
(3)
P 		246
B 186.3
(-0.4)
P 		99.9
(-2.3)
P 		69.8
(-3)
P 		333.9
(3)
P 		264
C 272.8
(3)
P 		129.4
(-1.1)
P 		64
(-3)
P 		271.7
(3)
P 		209
avr 212.5
(0.6) 		106.6
(-2.1) 		70.9
(-3) 		310.1
(3) 		239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 122.2
(2.5)
P 		128.1
(3)
P 		103.8
(0.8)
P 		68.8
(-2.4)
P 		98.1
(0.3)
P 		61.6
(-3)
P 		67
B 145.9
(1.1)
P 		180.7
(3)
P 		98.5
(-1.4)
P 		82
(-2.3)
P 		112.3
(-0.7)
P 		69.2
(-3)
P 		112
C 188.4
(3)
P 		119.6
(-0.6)
P 		106.5
(-1.3)
P 		117.3
(-0.7)
P 		73.9
(-3)
P 		91.7
(-2.1)
P 		115
avr 152.2
(3) 		142.8
(2.3) 		102.9
(-0.8) 		89.4
(-1.8) 		94.8
(-1.4) 		74.2
(-3) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 65
(3)
P 		19.6
(-3)
P 		20.6
(-2.9)
A 		47.5
(0.7)
P 		45
B 59.4
(1.7)
P 		24.7
(-2.1)
P 		16.2
(-3)
P 		71.1
(3)
P 		55
C 90.3
(3)
P 		37.2
(-1.7)
P 		22.3
(-3)
P 		45.8
(-0.9)
P 		68
avr 71.6
(3) 		27.2
(-2.1) 		19.7
(-3) 		54.8
(1.1) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 22.5
(-1.5)
P 		45.6
(3)
P 		22.6
(-1.5)
P 		17.5
(-2.5)
P 		29.6
(-0.1)
P 		15
(-3)
P 		31
B 50.8
(1.5)
P 		60.1
(3)
P 		23.9
(-2.7)
P 		21.7
(-3)
P 		45.6
(0.7)
P 		25.8
(-2.4)
P 		38
C 69.8
(3)
P 		36.5
(-1.3)
P 		27.1
(-2.5)
P 		23.6
(-3)
P 		23.9
(-3)
P 		30.7
(-2.1)
P 		46
avr 47.7
(3) 		47.4
(2.9) 		24.5
(-2.2) 		20.9
(-3) 		33
(-0.3) 		23.8
(-2.4) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190235_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12434
Sequence Type Exemplar sequence
Representative Public ID E02C12.5 NCBI
Target Description E02C12.5 /REP_DB=WormBase Gene ID /WP=CE04314 /GEN=gpa-3 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073024 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-3) (gpa-3) mRNA, complete cds. 11/11 None
SNAP00000032004 ENSEMBL cdna:SNAP chromosome:CEL140:V:9360319:9362334:-1 11/11 None
GENEFINDER00000032020 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9357153:9362334:-1 11/11 None
E02C12.5 ENSEMBL cdna:known chromosome:CEL140:V:9359334:9362334:-1 gene:E02C12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9360318-9362334(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-3
Ensembl E02C12.5
Entrez Gene 179319 Entrez gene
SwissProt P28052 EMBL-EBI
WormBase CE04314 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83462 G protein, alpha subunit protein 3 [Caenorhabditis elegans] ref|NP_505425.1| G Protein, Alpha subunit family member (gpa-3) [Caenorhabditis elegans] gb|AAG32079.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|P28052|GPA3_CAEEL Guanine nucleotide-binding protein alpha-3 subunit 0.0
blast AAA28061 G protein alpha subunit 0.0
blast AAW02902 gpa-3 [Caenorhabditis briggsae] sp|Q60W52|GPA3_CAEBR Guanine nucleotide-binding protein alpha-3 subunit emb|CAE72163.1| Hypothetical protein CBG19263 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001534 EMBL-EBI Transthyretin-like 5.7E-46
Pfam IPR001534 EMBL-EBI Transthyretin-like 2.8E-54
Sequence
Target Sequence 
>C. ELEGANS:190235_AT
gcctgaaagtgcaccacattttctatcaagtcttgatcgtatcaagttacccgattataa
tccaactgaacaggatattcttctgtctagaatcaagacaactggaattgttgaagttaa
atttcaaatgaaaagtgtagattttagggtatttgacgttggaggtcaacgatcagagag
aaagaaatggattcattgttttgaagatgttaatgcaattattttcatcgctgcaatttc
agaatatgatcaagttctgtttgaagatgagacgacgaatcgaatgattgaatcaatgag
gctgtttgaatcaatttgtaattcacgatggttcatcaatacttcaatgattcttttctt
aaataagaaggatttgtttgccgagaagattaaaagaacttcaattaaatccgcattccc
agactataaaggtgcacaaacctacgacgaatcgtgtcgatatattgaggaaaagtttga
tggattgaatgcaaatccagaaaagacgatttacatgcatcagacgtgtgcaacagatac
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCTGAAAGTGCACCACATTTTCTA 372 283 459 Antisense
GTTACCCGATTATAATCCAACTGAA 8 447 504 Antisense
GAACAGGATATTCTTCTGTCTAGAA 630 351 526 Antisense
ATTATTTTCATCGCTGCAATTTCAG 654 13 676 Antisense
AATACTTCAATGATTCTTTTCTTAA 13 171 796 Antisense
GAAGGATTTGTTTGCCGAGAAGATT 22 335 825 Antisense
GAACTTCAATTAAATCCGCATTCCC 555 349 854 Antisense
AAATCCGCATTCCCAGACTATAAAG 529 117 865 Antisense
GTGCACAAACCTACGACGAATCGTG 307 481 890 Antisense
AAGACGATTTACATGCATCAGACGT 664 153 961 Antisense
GCATCAGACGTGTGCAACAGATACA 515 309 975 Antisense
Sequence Source Affymetrix Proprietary Database