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[190231_at] threonine dehydratase

Description T01H8.2 /REP_DB=WormBase Gene ID /WP=CE13016 /TR=Q93968 /GB=CAB02298.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=threonine dehydratase
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 42      6grp_total > 31      4grp_total > 58      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 721
1 2 3 4 range
A 645.6
(0.2)
P 		920.2
(3)
P 		919.6
(3)
P 		329.8
(-3)
P 		590
B 556.1
(-0.4)
P 		892.4
(2.7)
P 		920.4
(3)
P 		275.6
(-3)
P 		645
C 563.6
(-1.2)
P 		970.6
(2.7)
P 		996.9
(3)
P 		384.6
(-3)
P 		612
avr 588.4
(-0.5) 		927.7
(2.8) 		945.6
(3) 		330
(-3) 		616

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1067
1 2 3 4 5 6 range
A 430.5
(-2.4)
P 		968.7
(0.6)
P 		1399.7
(3)
P 		946.5
(0.5)
P 		346.2
(-2.9)
P 		332.3
(-3)
P 		1067
B 634.4
(-1.3)
P 		1023.6
(1)
P 		1377.2
(3)
P 		735.5
(-0.7)
P 		482.3
(-2.2)
P 		337.6
(-3)
P 		1040
C 673.8
(-0.3)
P 		987.7
(2.4)
P 		1061.2
(3)
P 		825.7
(1)
P 		464.7
(-2.1)
P 		362.6
(-3)
P 		699
avr 579.6
(-1.5) 		993.3
(1.2) 		1279.4
(3) 		835.9
(0.2) 		431.1
(-2.4) 		344.2
(-3) 		935
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 235.1
(2.8)
P 		199.8
(1.7)
P 		240.4
(3)
P 		48.3
(-3)
P 		192
B 177.3
(1.4)
P 		220.4
(3)
P 		213.3
(2.7)
P 		58.7
(-3)
P 		162
C 186.6
(-0.4)
P 		279
(1.5)
P 		347.5
(3)
P 		64.9
(-3)
P 		283
avr 199.7
(1.1) 		233.1
(2) 		267.1
(3) 		57.3
(-3) 		210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 79.4
(-3)
P 		344.5
(3)
P 		304.8
(2.1)
P 		240
(0.6)
P 		104.5
(-2.4)
P 		80.9
(-3)
P 		265
B 220.7
(-0.4)
P 		340.7
(3)
P 		334.4
(2.8)
P 		194.8
(-1.1)
P 		195.9
(-1)
P 		125.9
(-3)
P 		215
C 249.7
(1.3)
P 		301.4
(3)
P 		270.1
(2)
P 		166
(-1.5)
P 		150.4
(-2)
P 		121.4
(-3)
P 		180
avr 183.3
(-1) 		328.9
(3) 		303.1
(2.3) 		200.3
(-0.5) 		150.3
(-1.9) 		109.4
(-3) 		220
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190231_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.811
Sequence Type Exemplar sequence
Representative Public ID T01H8.2 NCBI
Target Description T01H8.2 /REP_DB=WormBase Gene ID /WP=CE13016 /TR=Q93968 /GB=CAB02298.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=threonine dehydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059917(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059917 NCBI Caenorhabditis elegans T01H8.2 (T01H8.2) mRNA, complete cds. 11/11 None
GENEFINDER00000010903 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8573245:8581872:-1 11/11 None
SNAP00000010900 ENSEMBL cdna:SNAP chromosome:CEL140:I:8579749:8581872:-1 11/11 None
T01H8.2 ENSEMBL cdna:known chromosome:CEL140:I:8579749:8581872:-1 gene:T01H8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8579679-8581803(-) 99.37 100.0
Public Domain and Genome References
Gene Title threonine dehydratase
Ensembl T01H8.2
Entrez Gene 187970 Entrez gene
SwissProt Q93968 EMBL-EBI
WormBase CE13016 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16829 lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02298 Hypothetical protein T01H8.2 [Caenorhabditis elegans] ref|NP_492318.1| T01H8.2 [Caenorhabditis elegans] 1.0E-171
blast CAB02298 Hypothetical protein T01H8.2 [Caenorhabditis elegans] ref|NP_492318.1| T01H8.2 [Caenorhabditis elegans] 1.0E-169
blast CAB02298 Hypothetical protein T01H8.2 [Caenorhabditis elegans] ref|NP_492318.1| T01H8.2 [Caenorhabditis elegans] 1.0E-157
blast CAE67104 Hypothetical protein CBG12517 [Caenorhabditis briggsae] 1.0E-128
blast CAE68099 Hypothetical protein CBG13739 [Caenorhabditis briggsae] 1.0E-124
blast CAE68099 Hypothetical protein CBG13739 [Caenorhabditis briggsae] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.1E-79
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 5.0E-65
Sequence
Target Sequence 
>C. ELEGANS:190231_AT
gtgagccaactgttactagtcgagaatctgtatgtgctgatttgactgaaaaacttgagt
attattgtgtagaaccttataattgtgtttcaatgatcaacggacattcatcagttgcat
ttgaaatcctggaacaggttggcaatgagattgattccatttttttatctgttggaggtg
gtggtttagcttcatctgttgcttttctgataggcaacttgaggccagacatcgaagtat
accttgtgcaaccagcacagaaagaactttcgaactttctggaaaatggaattaagtgtc
cggttgacacattggatacaattgctgatggagttcgtgtagcaagagttggaccgcttt
gtgaaccaatactcaaacagtattgtgcacaaaaagttatcagtgtgaaggaagacgaga
tcaaagaaggattgaaactcatatggacaagaatgaagcaacgaattgaaccaactgcag
cacttgcatttgcaggagttctctaccacaaaccggctcacatcaaacgtccactagtca
tcctatgcggagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAGCCAACTGTTACTAGTCGAGA 419 483 383 Antisense
AACGGACATTCATCAGTTGCATTTG 496 143 481 Antisense
GTGGTGGTTTAGCTTCATCTGTTGC 393 489 560 Antisense
GGCAACTTGAGGCCAGACATCGAAG 305 535 595 Antisense
GAAGTATACCTTGTGCAACCAGCAC 614 335 616 Antisense
GTAGCAAGAGTTGGACCGCTTTGTG 701 455 721 Antisense
GGACCGCTTTGTGAACCAATACTCA 350 521 733 Antisense
GAATTGAACCAACTGCAGCACTTGC 32 325 845 Antisense
TGCAGGAGTTCTCTACCACAAACCG 324 577 873 Antisense
CTCACATCAAACGTCCACTAGTCAT 535 227 899 Antisense
GTCCACTAGTCATCCTATGCGGAGG 545 477 911 Antisense
Sequence Source Affymetrix Proprietary Database