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[190229_at] peptidase

Description T20F5.2 /REP_DB=WormBase Gene ID /WP=CE13818 /GEN=pbs-4 /TR=P91477 /GB=AAB37663.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 35      6grp_total > 63      4grp_total > 57      6grp_highest_sub > 03_04      6grp_cgc > 75      6grp_high1_sub > 03_04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2777
1 2 3 4 range
A 2693.1
(-2)
P
4434.7
(2.7)
P
4556.7
(3)
P
2341.8
(-3)
P
2215
B 2726.2
(-3)
P
4409
(1.2)
P
5118.3
(3)
P
2831.6
(-2.7)
P
2392
C 2705.7
(-3)
P
4551.6
(3)
P
4519.7
(2.9)
P
2990.5
(-2.1)
P
1846
avr 2708.3
(-3)
4465.1
(2.2)
4731.6
(3)
2721.3
(-3)
2023

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2247
1 2 3 4 5 6 range
A 2672.9
(-3)
P
2893.9
(-2.1)
P
4150.5
(3)
P
3813
(1.6)
P
3238.4
(-0.7)
P
2682.2
(-3)
P
1478
B 2058.2
(-3)
P
3108.4
(-0.2)
P
4305
(3)
P
4231.8
(2.8)
P
3309.5
(0.3)
P
3306.2
(0.3)
P
2247
C 2310.2
(-3)
P
3101.8
(-0.6)
P
3643.6
(1)
P
4296.5
(3)
P
2801
(-1.5)
P
3514.8
(0.6)
P
1986
avr 2347.1
(-3)
3034.7
(-0.7)
4033
(2.7)
4113.8
(3)
3116.3
(-0.4)
3167.7
(-0.2)
1767
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1232
1 2 3 4 range
A 980.6
(1.5)
P
962.7
(1.4)
P
1191.2
(3)
P
342.8
(-3)
P
848
B 869.4
(-0.3)
P
1088.9
(2)
P
1186
(3)
P
603.4
(-3)
P
583
C 895.7
(-0.8)
P
1308.6
(1.5)
P
1575.2
(3)
P
504.2
(-3)
P
1071
avr 915.2
(0.1)
1120.1
(1.6)
1317.5
(3)
483.5
(-3)
834

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 852
1 2 3 4 5 6 range
A 492.7
(-3)
P
1029.2
(3)
P
903.9
(1.6)
P
967.1
(2.3)
P
977.2
(2.4)
P
653
(-1.2)
P
537
B 716.1
(-3)
P
1034.7
(0)
P
1045.4
(0.1)
P
1120.9
(0.9)
P
1344.6
(3)
P
1232.9
(1.9)
P
629
C 856.3
(-3)
P
946.4
(-1.3)
P
927.4
(-1.7)
P
864
(-2.9)
P
906.4
(-2.1)
P
1176.6
(3)
P
320
avr 688.4
(-3)
1003.4
(1.9)
958.9
(1.2)
984
(1.6)
1076.1
(3)
1020.8
(2.1)
388
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190229_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.284
Sequence Type Exemplar sequence
Representative Public ID T20F5.2 NCBI
Target Description T20F5.2 /REP_DB=WormBase Gene ID /WP=CE13818 /GEN=pbs-4 /TR=P91477 /GB=AAB37663.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058860(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_058860 NCBI Caenorhabditis elegans T20F5.2 (peptidase) mRNA, complete cds. 11/11 None
SNAP00000034146 ENSEMBL cdna:SNAP chromosome:CEL140:I:3908754:3909516:1 11/11 None
GENEFINDER00000034156 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3908754:3909516:1 11/11 None
T20F5.2 ENSEMBL cdna:known chromosome:CEL140:I:3908743:3909627:1 gene:T20F5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3908753-3909516(+) 100.0 100.0
Public Domain and Genome References
Gene Title peptidase
Gene Symbol pbs-4
Ensembl T20F5.2
Entrez Gene 171975 Entrez gene
SwissProt P91477 EMBL-EBI
WormBase CE13818 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256939_AT 20S proteasome beta subunit D (PBD1) (PRGB) at
CANINE:1601594_AT similar to Proteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I) cfa
CANINE_2:CFA.790.1.S1_S_AT similar to Proteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I) cfa
DROSOPHILA_2:1626782_AT dm
DROSGENOME1:146372_AT dm
CHICKEN:GGA.8492.1.S1_AT proteasome (prosome, macropain) subunit, beta type, 2 gga
HG-U95AV2:1310_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HC-G110:1310_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
PLASMODIUM_ANOPHELES:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133_PLUS_2:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133A:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133B:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-FOCUS:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133A_2:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
U133_X3P:200039_S_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133A:201404_X_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133_PLUS_2:201404_X_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U133A_2:201404_X_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HUGENEFL:D26599_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
U133_X3P:G4506194_3P_A_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
U133_X3P:G12653014_3P_X_AT proteasome (prosome, macropain) subunit, beta type, 2 hs
HU35KSUBC:RC_AA416733_AT Proteasome (prosome, macropain) subunit, beta type, 2 hs
HG-U95C:63021_AT Proteasome (prosome, macropain) subunit, beta type, 2 hs
MU11KSUBA:AA683747_S_AT proteasome (prosome, macropain) subunit, beta type 2 mm
MG-U74AV2:94219_AT proteasome (prosome, macropain) subunit, beta type 2 mm
MOE430A:1448262_AT proteasome (prosome, macropain) subunit, beta type 2 mm
MOUSE430A_2:1448262_AT proteasome (prosome, macropain) subunit, beta type 2 mm
MOUSE430_2:1448262_AT proteasome (prosome, macropain) subunit, beta type 2 mm
MOE430B:1444377_AT Proteasome (prosome, macropain) subunit, beta type 2, mRNA (cDNA clone MGC:6558 IMAGE:2811010) mm
MOUSE430_2:1444377_AT Proteasome (prosome, macropain) subunit, beta type 2, mRNA (cDNA clone MGC:6558 IMAGE:2811010) mm
MOE430B:1459641_AT Proteasome (prosome, macropain) subunit, beta type 2, mRNA (cDNA clone MGC:6558 IMAGE:2811010) mm
MOUSE430_2:1459641_AT Proteasome (prosome, macropain) subunit, beta type 2, mRNA (cDNA clone MGC:6558 IMAGE:2811010) mm
MU19KSUBC:TC36059_AT Proteasome (prosome, macropain) subunit, beta type 2, mRNA (cDNA clone MGC:6558 IMAGE:2811010) mm
RT-U34:D21799_AT proteasome (prosome, macropain) subunit, beta type 2 rn
RG-U34A:D21799_AT proteasome (prosome, macropain) subunit, beta type 2 rn
RT-U34:D21799_G_AT proteasome (prosome, macropain) subunit, beta type 2 rn
RG-U34A:D21799_G_AT proteasome (prosome, macropain) subunit, beta type 2 rn
RAE230A:1398786_AT proteasome (prosome, macropain) subunit, beta type 2 rn
RAT230_2:1398786_AT proteasome (prosome, macropain) subunit, beta type 2 rn
YEAST_2:1774713_AT 20S proteasome beta-type subunit; localizes to the nucleus throughout the cell cycle Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5839 proteasome core complex (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4175 endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37663 Proteasome beta subunit protein 4 [Caenorhabditis elegans] ref|NP_491261.1| T20F5.2 [Caenorhabditis elegans] pir||T29206 hypothetical protein T20F5.2 - Caenorhabditis elegans 1.0E-115
blast P91477 Proteasome subunit beta type 2 (Proteasome subunit beta 4) 1.0E-113
blast CAE66696 Hypothetical protein CBG12037 [Caenorhabditis briggsae] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 1.7E-33
Sequence
Target Sequence
>C. ELEGANS:190229_AT
aaactacgtgattttggccgcagacaaagccactttcgcttatggcgctattctcgctga
cagcgaaaacgacaaggaatatagactgggaaagaagttgacaatgatgtgcatcggaga
ggaaggagacgtcgcacagttcggagactggaccaagagaaatcttcagttgtactcggt
cagaaatggttacgaggtttcaccatcatgtgctcatcacttcgttcggcgcagtatcgc
cgaaggtctccgctcgcaagatcactacactgtcgatgttctgattggaggatatgatga
caaggaggacaaggctttcctaggatctgttgattacttggctaatggactcgggcaaca
gccgtacttgttccgaggattctgcggacgcttctgctatgccattatggatcgtgaata
caaaaaagacatgactgaggctgaaggactcgctttgatgaacaagtgcatcggagaggc
taagagacgtttcgtcgccaacattcc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
AAACTACGTGATTTTGGCCGCAGAC 628 127 51 Antisense
CAAAGCCACTTTCGCTTATGGCGCT 162 193 75 Antisense
TTATGGCGCTATTCTCGCTGACAGC 62 681 90 Antisense
AGGAGACGTCGCACAGTTCGGAGAC 260 55 174 Antisense
GAGGTTTCACCATCATGTGCTCATC 183 403 244 Antisense
TCACTTCGTTCGGCGCAGTATCGCC 210 627 267 Antisense
AGATCACTACACTGTCGATGTTCTG 238 65 309 Antisense
AATGGACTCGGGCAACAGCCGTACT 150 161 394 Antisense
GCCGTACTTGTTCCGAGGATTCTGC 483 283 411 Antisense
ACGCTTCTGCTATGCCATTATGGAT 432 91 438 Antisense
AGAGACGTTTCGTCGCCAACATTCC 169 69 533 Antisense
Sequence Source Affymetrix Proprietary Database