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[190209_at]

Description ZK250.3 /REP_DB=WormBase Gene ID /WP=CE15271 /TR=O17300 /GB=AAB71072.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse trascriptase
Cluster 6grp_total > 0      4grp_total > 19      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 2.3
(-2)
A 		1.3
(-3)
A 		1.3
(-3)
A 		7.2
(3)
A 		6
B 1.4
(-1.9)
A 		2.2
(0.9)
A 		1.1
(-3)
A 		2.8
(3)
A 		2
C 2.1
(-2.3)
A 		5.1
(0.4)
A 		1.3
(-3)
A 		8.1
(3)
A 		7
avr 1.9
(-2.1) 		2.9
(-0.9) 		1.2
(-3) 		6
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.4
(-2.5)
A 		3.5
(-0.3)
A 		0.9
(-3)
A 		1.7
(-2.2)
A 		6.6
(3)
A 		3.2
(-0.6)
A 		6
B 1.3
(-3)
A 		2.8
(0.1)
A 		2.4
(-0.7)
A 		1.3
(-3)
A 		4.2
(3)
A 		1.7
(-2.2)
A 		3
C 2.1
(-2.1)
A 		8.6
(3)
A 		3.1
(-1.3)
A 		0.9
(-3)
A 		2.1
(-2.1)
A 		2.9
(-1.4)
A 		8
avr 1.6
(-2.5) 		5
(3) 		2.1
(-1.7) 		1.3
(-3) 		4.3
(1.9) 		2.6
(-0.9) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 0.8
(0.8)
A 		0.3
(-3)
A 		0.3
(-3)
A 		1.1
(3)
A 		1
B 0.5
(1)
A 		0.6
(3)
A 		0.3
(-3)
A 		0.6
(3)
A 		0
C 0.7
(-1.4)
A 		1.5
(3)
A 		0.4
(-3)
A 		1.4
(2.5)
A 		1
avr 0.7
(0.4) 		0.8
(1.3) 		0.3
(-3) 		1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 0.3
(-2.7)
A 		1.2
(0.3)
A 		0.2
(-3)
A 		0.4
(-2.3)
A 		2
(3)
A 		0.8
(-1)
A 		2
B 0.5
(-2.1)
A 		0.9
(-0.4)
A 		0.6
(-1.7)
A 		0.3
(-3)
A 		1.7
(3)
A 		0.6
(-1.7)
A 		1
C 0.8
(-1.5)
A 		2.6
(3)
A 		0.8
(-1.5)
A 		0.2
(-3)
A 		0.7
(-1.8)
A 		1
(-1)
A 		2
avr 0.5
(-2.1) 		1.6
(3) 		0.5
(-2.1) 		0.3
(-3) 		1.5
(2.5) 		0.8
(-0.7) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190209_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4576
Sequence Type Exemplar sequence
Representative Public ID ZK250.3 NCBI
Target Description ZK250.3 /REP_DB=WormBase Gene ID /WP=CE15271 /TR=O17300 /GB=AAB71072.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=reverse trascriptase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061734(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061734 NCBI Caenorhabditis elegans ZK250.3 (ZK250.3) mRNA, complete cds. 11/11 None
ZK250.3 ENSEMBL cdna:known chromosome:CEL140:II:1941948:1943107:1 gene:ZK250.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1941946-1943106(+) 100.0 100.0
chrV:9772689-9773596(-) 84.59 84.59
Public Domain and Genome References
Ensembl ZK250.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494135 ZK250.3 [Caenorhabditis elegans] 0.0
blast NP_509377 C17H11.3 [Caenorhabditis elegans] 6.0E-93
blast NP_498860 ZK1236.4 [Caenorhabditis elegans] sp|P34620|YO84_CAEEL Hypothetical protein ZK1236.4 in chromosome III 6.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.0E-22
Sequence
Target Sequence 
>C. ELEGANS:190209_AT
caacgacacgtgacttaggctttatgatctctcataatctggattttagtgaacactgga
aaaaggctattaattcttctaaatttcaaatgacacaaatttttaaccattataacagta
acaaaccaaatctgatattcctattatataaaacatttattaggcctttgctagagtacg
gaaccgttataagtagcccaccaaagcagtcagatataagagccatcgaggcagttcaaa
attcgttcacaaggcgtctatatagtaggactgtaggcaaatacgtctcaataagtgatc
cggaatataaaacctcggcagaaagaaatgaattgtttgggctcacgacgctgcaaagac
gacgtctgacaaccgataaaaaaatgatttcgaaaatgataaacggtaaaatagatctga
aagtacctgaattttttaccttctcggacaacactagaacgcgttgtaaaagaaagttct
cttggagcagatgtaaatctaaaattaggaaacatttctttgttaacagaatattaagcc
aaccggcattcctcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACGACACGTGACTTAGGCTTTAT 476 187 464 Antisense
GGCCTTTGCTAGAGTACGGAACCGT 483 535 626 Antisense
GTAGCCCACCAAAGCAGTCAGATAT 99 457 656 Antisense
GAGGCAGTTCAAAATTCGTTCACAA 576 405 691 Antisense
AATTCGTTCACAAGGCGTCTATATA 303 177 703 Antisense
GGACTGTAGGCAAATACGTCTCAAT 412 523 731 Antisense
ATGAATTGTTTGGGCTCACGACGCT 141 41 791 Antisense
GCTCACGACGCTGCAAAGACGACGT 228 301 804 Antisense
AAGACGACGTCTGACAACCGATAAA 305 119 819 Antisense
TTTTTACCTTCTCGGACAACACTAG 481 671 896 Antisense
ATTAAGCCAACCGGCATTCCTCAAA 239 11 996 Antisense
Sequence Source Affymetrix Proprietary Database