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[190203_at] calpain-like protease

Description F44F1.3 /REP_DB=WormBase Gene ID /WP=CE24976 /TR=O02260 /GB=CAB03101.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain-like protease
Cluster 6grp_total > 97      4grp_total > 10      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 9.9
(-1.2)
A 		30.2
(3)
P 		15.7
(0)
A 		1
(-3)
A 		29
B 13.6
(0.1)
A 		2.6
(-3)
A 		16.7
(1)
A 		23.9
(3)
M 		21
C 18
(-1.6)
A 		10.8
(-2.8)
A 		9.8
(-3)
A 		44
(3)
P 		34
avr 13.8
(-3) 		14.5
(-2.5) 		14.1
(-2.8) 		23
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 1.2
(-3)
A 		14.2
(-1.2)
A 		7.2
(-2.2)
A 		5.5
(-2.4)
A 		35.6
(1.7)
M 		45.4
(3)
P 		44
B 0.9
(-3)
A 		12.6
(1.3)
A 		2.4
(-2.5)
A 		8
(-0.4)
A 		17.3
(3)
A 		11.5
(0.9)
A 		16
C 1.1
(-3)
A 		11.3
(-0.8)
A 		1.8
(-2.9)
A 		17.6
(0.5)
A 		11.8
(-0.7)
A 		29.1
(3)
P 		28
avr 1.1
(-3) 		12.7
(-0.5) 		3.8
(-2.4) 		10.4
(-1) 		21.6
(1.5) 		28.7
(3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 3.6
(0.3)
A 		6.5
(3)
P 		4.1
(0.8)
A 		0.1
(-3)
A 		6
B 4.3
(1.9)
A 		0.6
(-3)
A 		3.9
(1.4)
A 		5.1
(3)
M 		5
C 6
(1)
A 		3.1
(-3)
A 		3.4
(-2.6)
A 		7.4
(3)
P 		4
avr 4.6
(3) 		3.4
(-3) 		3.8
(-1) 		4.2
(1) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.2
(-3)
A 		5.1
(-0.3)
A 		1.6
(-2.2)
A 		1.4
(-2.3)
A 		10.7
(2.8)
M 		11.1
(3)
P 		11
B 0.3
(-3)
A 		4.2
(0.5)
A 		0.6
(-2.7)
A 		2.1
(-1.4)
A 		7
(3)
A 		4.3
(0.6)
A 		7
C 0.4
(-3)
A 		3.4
(-1.1)
A 		0.5
(-2.9)
A 		3.5
(-1)
A 		3.8
(-0.8)
A 		9.7
(3)
P 		9
avr 0.3
(-3) 		4.2
(-0.1) 		0.9
(-2.6) 		2.3
(-1.5) 		7.2
(2.1) 		8.4
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190203_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2235
Sequence Type Exemplar sequence
Representative Public ID F44F1.3 NCBI
Target Description F44F1.3 /REP_DB=WormBase Gene ID /WP=CE24976 /TR=O02260 /GB=CAB03101.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain-like protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060926(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060926 NCBI Caenorhabditis elegans F44F1.3 (F44F1.3) mRNA, complete cds. 11/11 None
SNAP00000015369 ENSEMBL cdna:SNAP chromosome:CEL140:I:13262260:13265786:1 11/11 None
GENEFINDER00000015378 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13262260:13265925:1 11/11 None
F44F1.3 ENSEMBL cdna:known chromosome:CEL140:I:13262260:13265786:1 gene:F44F1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13262190-13265717(+) 100.0 100.0
Public Domain and Genome References
Gene Title calpain-like protease
Ensembl F44F1.3
Entrez Gene 185746 Entrez gene
SwissProt O02260 EMBL-EBI
WormBase CE36160 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03101 Hypothetical protein F44F1.3 [Caenorhabditis elegans] ref|NP_493327.2| F44F1.3 [Caenorhabditis elegans] 0.0
blast NP_493325 F44F1.1 [Caenorhabditis elegans] 0.0
blast CAC42267 Hypothetical protein T11A5.6 [Caenorhabditis elegans] emb|CAA96686.2| Hypothetical protein T11A5.6 [Caenorhabditis elegans] ref|NP_741604.1| T11A5.6 [Caenorhabditis elegans] 6.0E-54
blast CAE72273 Hypothetical protein CBG19393 [Caenorhabditis briggsae] 1.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.5E-6
Sequence
Target Sequence 
>C. ELEGANS:190203_AT
aagtagttgcatgtgagatcatcaacgattctactcaggatatcgatttagaacctggta
tatacactatcacagtcatttatctcaatgactggagcttggacaaacgagacatctcaa
ttcactcttcccgaccaatttcggtcaatgaagggttttcgagaacaaggaaagatagcg
tacatataaaatatatagtggataagtttgggcaagaaattgtgaaagagcagaaggata
aaatgagcattaagaagtacactgataataatttcacattcatcgcagtagttgcatgga
acttcacatatgatcagtttctacatgctcatttgcgttactcaatcactgatgaacaat
ttgtctctagaagtttagaagacaagcaaacagttgatgttttggttgtgataaatcttg
aagcaatgcctgaaatagtggatttcccaattgacattgattataaactttcaaaagatg
ttgatgcaacaatcaatggaatcaaaagaggatgccaccttccagaaatatcttcatctc
gtttcttagaatatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTAGTTGCATGTGAGATCATCAA 19 151 1304 Antisense
GATCATCAACGATTCTACTCAGGAT 262 421 1320 Antisense
AGGATATCGATTTAGAACCTGGTAT 162 57 1340 Antisense
AGAACCTGGTATATACACTATCACA 362 73 1353 Antisense
CACTATCACAGTCATTTATCTCAAT 461 197 1368 Antisense
TATCTCAATGACTGGAGCTTGGACA 74 653 1384 Antisense
GGAGCTTGGACAAACGAGACATCTC 277 521 1397 Antisense
ATTTCACATTCATCGCAGTAGTTGC 684 13 1574 Antisense
GATAAATCTTGAAGCAATGCCTGAA 254 423 1713 Antisense
AGGATGCCACCTTCCAGAAATATCT 449 57 1812 Antisense
TCTTCATCTCGTTTCTTAGAATATG 374 617 1834 Antisense
Sequence Source Affymetrix Proprietary Database