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[190189_at] threonine dehydratase

Description K01C8.1 /REP_DB=WormBase Gene ID /WP=CE02261 /TR=Q21080 /GB=CAA88854.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=threonine dehydratase
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 43      6grp_total > 63      4grp_total > 14      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 862
1 2 3 4 range
A 1184.2
(1.4)
P 		776.1
(-2.2)
P 		690.1
(-3)
P 		1357.8
(3)
P 		668
B 1242.8
(1.6)
P 		813.7
(-1.5)
P 		609.5
(-3)
P 		1434.2
(3)
P 		825
C 1338.2
(2.6)
P 		571.8
(-3)
P 		682.4
(-2.2)
P 		1387.5
(3)
P 		816
avr 1255.1
(1.9) 		720.5
(-2.5) 		660.7
(-3) 		1393.2
(3) 		733

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1294
1 2 3 4 5 6 range
A 652.8
(-2.5)
P 		1007
(-0.7)
P 		1713.4
(3)
P 		1551
(2.2)
P 		552.4
(-3)
P 		982.1
(-0.8)
P 		1161
B 795.9
(-2.5)
P 		827.5
(-2.3)
P 		1628.8
(3)
P 		1346.1
(1.1)
P 		714.7
(-3)
P 		800.2
(-2.4)
P 		914
C 812.9
(-2.9)
P 		800.1
(-3)
P 		1846.1
(3)
P 		1471.4
(0.9)
P 		806.4
(-3)
P 		965.2
(-2.1)
P 		1046
avr 753.9
(-2.6) 		878.2
(-1.9) 		1729.4
(3) 		1456.2
(1.4) 		691.2
(-3) 		915.8
(-1.7) 		1038
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 431.2
(3)
P 		168.5
(-3)
P 		180.4
(-2.7)
P 		198.7
(-2.3)
P 		263
B 396.3
(3)
P 		201
(-1.6)
P 		141.2
(-3)
P 		305.6
(0.9)
P 		255
C 443
(3)
P 		164.4
(-3)
P 		237.8
(-1.4)
P 		234
(-1.5)
P 		279
avr 423.5
(3) 		178
(-3) 		186.5
(-2.8) 		246.1
(-1.3) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 350
1 2 3 4 5 6 range
A 120.3
(-3)
P 		358.2
(2.2)
P 		373.1
(2.6)
P 		393.4
(3)
P 		166.7
(-2)
P 		239.1
(-0.4)
P 		273
B 276.9
(-2.9)
P 		275.4
(-3)
P 		395.5
(3)
P 		356.5
(1.1)
P 		290.4
(-2.3)
P 		298.4
(-1.9)
P 		120
C 301.3
(-1.5)
P 		244.1
(-3)
P 		469.9
(3)
P 		295.9
(-1.6)
P 		260.9
(-2.6)
P 		323.1
(-0.9)
P 		226
avr 232.8
(-3) 		292.6
(-1) 		412.8
(3) 		348.6
(0.9) 		239.3
(-2.8) 		286.9
(-1.2) 		180
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190189_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3700
Sequence Type Exemplar sequence
Representative Public ID K01C8.1 NCBI
Target Description K01C8.1 /REP_DB=WormBase Gene ID /WP=CE02261 /TR=Q21080 /GB=CAA88854.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=threonine dehydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063340(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063340 NCBI Caenorhabditis elegans K01C8.1 (K01C8.1) mRNA, complete cds. 11/11 None
SNAP00000019054 ENSEMBL cdna:SNAP chromosome:CEL140:II:8265125:8267176:1 11/11 None
GENEFINDER00000019072 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8265125:8267176:1 11/11 None
K01C8.1.3 ENSEMBL cdna:known chromosome:CEL140:II:8264584:8267268:1 gene:K01C8.1 11/11 None
K01C8.1.1 ENSEMBL cdna:known chromosome:CEL140:II:8265110:8267268:1 gene:K01C8.1 11/11 None
K01C8.1.2 ENSEMBL cdna:known chromosome:CEL140:II:8265112:8267268:1 gene:K01C8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8265121-8267173(+) 100.0 100.0
Public Domain and Genome References
Gene Title threonine dehydratase
Ensembl K01C8.1
Entrez Gene 174326 Entrez gene
SwissProt Q21080 EMBL-EBI
WormBase CE36943 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1637971_A_AT dm
DROSGENOME1:141406_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4794 threonine ammonia-lyase activity inferred from electronic annotation QuickGO AmiGO
16597 amino acid binding inferred from electronic annotation QuickGO AmiGO
16829 lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88854 Hypothetical protein K01C8.1 [Caenorhabditis elegans] ref|NP_495741.2| K01C8.1 [Caenorhabditis elegans] 0.0
blast CAE57672 Hypothetical protein CBG00666 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002912 EMBL-EBI Amino acid-binding ACT 5.1E-9
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.8E-85
Pfam IPR002912 EMBL-EBI Amino acid-binding ACT 5.1E-9
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 2.4E-85
Sequence
Target Sequence 
>C. ELEGANS:190189_AT
tgaatcagaaacctgcccatcgttcactgaagcatatgaagctggccatataataacttc
ccaggccaaagcgtcacttgctgatggtctcgccgttcccacagtcggcggaaattcttt
ggagacagcaaaaggcttggttgataaggttatcacagtgaaagaagaaagcattgcgct
gagtattttaagacttttggaagttgaaaaggctgttgttgaaggaggaggagctgttgg
attagcagctattttggaaggaaaagtaccagaattgaaaggaaagaaagttgtttcaat
tctttccggtggaaatattgatacaacagtacttggaagatcaattgaaagaggattagc
tgtagatggaagacttgttcgtttagaagttgtcgtatcagatagaccaggaggtattgc
tgaactaacaactacaattgctcatcttggagcatcaatcaaagatattttccacgaaag
agcatggatctcaacggatgtattccacgttaaagttaaggttgtggcagaaactcgtgg
aa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATCAGAAACCTGCCCATCGTTC 112 575 945 Antisense
CTGCCCATCGTTCACTGAAGCATAT 202 237 957 Antisense
TAATAACTTCCCAGGCCAAAGCGTC 247 187 995 Antisense
AAGCGTCACTTGCTGATGGTCTCGC 3 151 1013 Antisense
CGCCGTTCCCACAGTCGGCGGAAAT 190 229 1035 Antisense
AGCATTGCGCTGAGTATTTTAAGAC 639 75 1114 Antisense
TGTTTCAATTCTTTCCGGTGGAAAT 479 559 1236 Antisense
GTTCGTTTAGAAGTTGTCGTATCAG 627 441 1321 Antisense
ACAACTACAATTGCTCATCTTGGAG 707 111 1372 Antisense
GGATCTCAACGGATGTATTCCACGT 8 511 1430 Antisense
AAGGTTGTGGCAGAAACTCGTGGAA 81 161 1462 Antisense
Sequence Source Affymetrix Proprietary Database