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[190181_at] ubiquitin hydrolase

Description H12I13.2 /REP_DB=WormBase Gene ID /WP=CE20962 /TR=Q9N5M8 /GB=AAF39880.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin hydrolase
Cluster 6grp_high1_10_10 > 30      6grp_total > 11      4grp_total > 19      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 33
(-3)
A 		36.8
(-2.2)
P 		46.2
(-0.2)
A 		61.4
(3)
P 		28
B 43.2
(0.8)
M 		57.9
(3)
P 		18.6
(-3)
A 		55.1
(2.6)
P 		39
C 45.8
(-1.2)
P 		56.8
(-0.1)
P 		28.1
(-3)
A 		87.4
(3)
P 		59
avr 40.7
(-1.4) 		50.5
(0.2) 		31
(-3) 		68
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 37
(-0.5)
A 		47.9
(0.6)
A 		71.4
(3)
P 		12.4
(-3)
A 		40.9
(-0.1)
P 		37.1
(-0.5)
A 		59
B 53.9
(3)
P 		41.9
(0.9)
P 		33.3
(-0.7)
M 		20.1
(-3)
A 		34.9
(-0.4)
P 		36.8
(-0)
P 		34
C 21.1
(-3)
A 		74.6
(3)
P 		47.2
(-0.1)
A 		55.4
(0.8)
P 		24.9
(-2.6)
A 		36.1
(-1.3)
P 		54
avr 37.3
(-1.1) 		54.8
(3) 		50.6
(2) 		29.3
(-3) 		33.6
(-2) 		36.7
(-1.3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12
(2.9)
A 		8
(-3)
P 		12.1
(3)
A 		9
(-1.5)
P 		4
B 13.8
(2.7)
M 		14.3
(3)
P 		4.3
(-3)
A 		11.8
(1.5)
P 		10
C 15.2
(2)
P 		16.3
(3)
P 		9.8
(-3)
A 		14.7
(1.5)
P 		7
avr 13.7
(3) 		12.9
(2) 		8.7
(-3) 		11.8
(0.7) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 6.8
(-1.4)
A 		17
(3)
A 		15.6
(2.4)
P 		3.2
(-3)
A 		12.3
(1)
P 		9
(-0.5)
A 		14
B 18.8
(3)
P 		14
(0.9)
P 		8.1
(-1.8)
M 		5.3
(-3)
A 		14.2
(1)
P 		13.7
(0.7)
P 		14
C 7.8
(-3)
A 		22.8
(3)
P 		12
(-1.3)
A 		11.1
(-1.7)
P 		8.1
(-2.9)
A 		12.1
(-1.3)
P 		15
avr 11.1
(-0.6) 		17.9
(3) 		11.9
(-0.2) 		6.5
(-3) 		11.5
(-0.4) 		11.6
(-0.3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4159
Sequence Type Exemplar sequence
Representative Public ID H12I13.2 NCBI
Target Description H12I13.2 /REP_DB=WormBase Gene ID /WP=CE20962 /TR=Q9N5M8 /GB=AAF39880.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin hydrolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062812(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062812 NCBI Caenorhabditis elegans H12I13.2 (H12I13.2) mRNA, complete cds. 11/11 None
SNAP00000034373 ENSEMBL cdna:SNAP chromosome:CEL140:II:6059759:6067093:-1 11/11 None
GENEFINDER00000034378 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6065959:6073205:-1 11/11 None
H12I13.2 ENSEMBL cdna:known chromosome:CEL140:II:6065959:6067093:-1 gene:H12I13.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6065955-6067090(-) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin hydrolase
Ensembl H12I13.2
Entrez Gene 186733 Entrez gene
SwissProt Q9N5M8 EMBL-EBI
WormBase CE20962 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39880 Hypothetical protein H12I13.2 [Caenorhabditis elegans] ref|NP_495213.1| H12I13.2 [Caenorhabditis elegans] 0.0
blast AAF39881 Hypothetical protein H12I13.1 [Caenorhabditis elegans] ref|NP_495212.1| H12I13.1 [Caenorhabditis elegans] 0.0
blast AAF39882 Hypothetical protein H12I13.3 [Caenorhabditis elegans] ref|NP_495214.1| H12I13.3 [Caenorhabditis elegans] 0.0
blast AAF39880 Hypothetical protein H12I13.2 [Caenorhabditis elegans] ref|NP_495213.1| H12I13.2 [Caenorhabditis elegans] 1.0E-172
blast AAK84500 Hypothetical protein C33E10.6 [Caenorhabditis elegans] ref|NP_510803.1| C33E10.6 [Caenorhabditis elegans] 4.0E-87
blast AAK31453 Hypothetical protein C25H3.8 [Caenorhabditis elegans] ref|NP_495110.2| C25H3.8 [Caenorhabditis elegans] 7.0E-79
blast AAO38631 Hypothetical protein F59E12.6b [Caenorhabditis elegans] ref|NP_871929.1| F59E12.6b [Caenorhabditis elegans] 8.0E-31
blast AAO38630 Hypothetical protein F59E12.6a [Caenorhabditis elegans] ref|NP_495095.2| F59E12.6a [Caenorhabditis elegans] 8.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.6E-11
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.5E-11
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.0E-12
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.6E-11
Sequence
Target Sequence 
>C. ELEGANS:190181_AT
caaagtggcactcatgtttcttctccgttcggtattcaccgatttgaggttttcggcaga
aagagcaattaatataacatcaatcataaaagttgttgaccaactttccccacatcctgt
gagtaatcaacaacaagatgccagcgagatgctccaaaacatcgtgatctggctgaaaga
cgcggtggacgctgcagtggctttccaacggattcatgtccgtgaatgtcatgaagacat
ccgtctgatggaacaaaagcttaaaattatcaaactacaagaagatcgttttgatagaat
attcgcatcgaatatgtatgaggagacgttgggacttcgggtgcacatccaaaagaacag
tcttcgcgaatccttcgagaaatttttctttgcaaatgatgcttattctgtggatgtggt
gatggtgcagattgtaagacttgcacaggacggaaggaaacttcacgacaattttctatt
tcctggaaatttagtgactgaatcttccgcgacgaaagagaagtgctacttcaagctgac
ctctgttatggttcatcgaggagaatgtgctcagtccggacac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGTGGCACTCATGTTTCTTCTC 413 193 366 Antisense
TTCTTCTCCGTTCGGTATTCACCGA 679 675 383 Antisense
ACTTTCCCCACATCCTGTGAGTAAT 267 103 468 Antisense
GAAAGACGCGGTGGACGCTGCAGTG 130 361 540 Antisense
GTGGCTTTCCAACGGATTCATGTCC 625 489 562 Antisense
GAGGAGACGTTGGGACTTCGGGTGC 585 399 685 Antisense
AAAGAACAGTCTTCGCGAATCCTTC 439 123 717 Antisense
TTAGTGACTGAATCTTCCGCGACGA 353 679 856 Antisense
GAAGTGCTACTTCAAGCTGACCTCT 264 335 885 Antisense
GCTGACCTCTGTTATGGTTCATCGA 29 295 900 Antisense
AGGAGAATGTGCTCAGTCCGGACAC 628 55 924 Antisense
Sequence Source Affymetrix Proprietary Database