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[190174_s_at] ATPase

Description F29G9.5 /REP_DB=WormBase Gene ID /WP=CE09799 /GEN=rpt-2 /TR=SW:O16368 /GB=AAB65906.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATPase
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 19      4grp_high_8_8 > 55      6grp_highest_nonorm > 56      6grp_total > 73      4grp_total > 56      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1373
1 2 3 4 range
A 1853.7
(-3)
P 		2145.4
(-1.5)
P 		3040.4
(3)
P 		1986.5
(-2.3)
P 		1187
B 1955.2
(-3)
P 		2482.9
(0.4)
P 		2881.6
(3)
P 		2053.9
(-2.4)
P 		926
C 1735.6
(-2.7)
P 		2385.4
(0.6)
P 		2864.1
(3)
P 		1667.2
(-3)
P 		1197
avr 1848.2
(-3) 		2337.9
(-0.3) 		2928.7
(3) 		1902.5
(-2.7) 		1081

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 737
1 2 3 4 5 6 range
A 2021.4
(-1.6)
P 		1938.2
(-3)
P 		1992.1
(-2.1)
P 		2163.7
(0.8)
P 		2290
(3)
P 		2251.6
(2.3)
P 		352
B 1884.9
(-2.2)
P 		1789.2
(-3)
P 		2526.2
(3)
P 		2323.7
(1.4)
P 		2298.8
(1.1)
P 		1883.3
(-2.2)
P 		737
C 2048.2
(-1.1)
P 		1827.4
(-3)
P 		2513.4
(3)
P 		2381
(1.8)
P 		2217.9
(0.4)
P 		2026.7
(-1.3)
P 		686
avr 1984.8
(-1.4) 		1851.6
(-3) 		2343.9
(3) 		2289.5
(2.3) 		2268.9
(2.1) 		2053.9
(-0.5) 		492
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 717
1 2 3 4 range
A 674.9
(1.6)
P 		465.7
(-0.9)
P 		794.8
(3)
P 		290.8
(-3)
P 		504
B 623.5
(1.8)
P 		613.2
(1.6)
P 		667.7
(3)
P 		437.7
(-3)
P 		230
C 574.5
(-0.5)
P 		685.8
(0.4)
P 		998.2
(3)
P 		281.1
(-3)
P 		717
avr 624.3
(0.6) 		588.2
(0.1) 		820.2
(3) 		336.5
(-3) 		484

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 561
1 2 3 4 5 6 range
A 372.6
(-3)
P 		689.3
(3)
P 		433.8
(-1.8)
P 		548.8
(0.3)
P 		691
(3)
P 		548.2
(0.3)
P 		318
B 655.9
(-1.9)
P 		595.6
(-3)
P 		613.4
(-2.7)
P 		615.5
(-2.6)
P 		934
(3)
P 		702.3
(-1.1)
P 		338
C 759.2
(3)
P 		557.6
(-1.3)
P 		639.7
(0.4)
P 		478.8
(-3)
P 		717.7
(2.1)
P 		678.5
(1.3)
P 		280
avr 595.9
(-1.8) 		614.2
(-1.3) 		562.3
(-2.6) 		547.7
(-3) 		780.9
(3) 		643
(-0.5) 		233
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190174_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12389
Sequence Type Exemplar sequence
Representative Public ID F29G9.5 NCBI
Target Description F29G9.5 /REP_DB=WormBase Gene ID /WP=CE09799 /GEN=rpt-2 /TR=SW:O16368 /GB=AAB65906.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072157 NCBI Caenorhabditis elegans F29G9.5 (ATPase) mRNA, complete cds. 11/11 A 
SNAP00000000271 ENSEMBL cdna:SNAP chromosome:CEL140:V:6018794:6020262:-1 11/11 None
GENEFINDER00000000285 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6018794:6020262:-1 11/11 None
F29G9.5.1 ENSEMBL cdna:known chromosome:CEL140:V:6018669:6020263:-1 gene:F29G9.5 11/11 None
F29G9.5.2 ENSEMBL cdna:known chromosome:CEL140:V:6018681:6020265:-1 gene:F29G9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6018793-6020262(-) 100.0 100.0
Public Domain and Genome References
Gene Title ATPase
Gene Symbol rpt-2
Ensembl F29G9.5
Entrez Gene 178988 Entrez gene
SwissProt O16368 EMBL-EBI
WormBase CE09799 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13606_AT 26S protease regulatory complex subunit 4, putative at
ATH1-121501:265595_AT 26S protease regulatory complex subunit 4, putative at
CANINE:1586075_S_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE:1598389_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE:1596843_X_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE:1586696_X_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE_2:CFA.6705.1.A1_S_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE_2:CFA.868.1.S1_S_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
CANINE_2:CFAAFFX.26768.1.S1_AT similar to peptidase (prosome, macropain) 26S subunit, ATPase 1 cfa
DROSOPHILA_2:1630788_AT Proteasome 26S subunit subunit 4 ATPase dm
DROSGENOME1:142982_AT Proteasome 26S subunit subunit 4 ATPase dm
CHICKEN:GGA.1071.1.S1_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 gga
HG-U95AV2:688_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HC-G110:688_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HG-U133_PLUS_2:204219_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HG-U133A:204219_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HG-FOCUS:204219_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HG-U133A_2:204219_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HUGENEFL:L02426_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
U133_X3P:G4506206_3P_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
U133_X3P:G4506206_3P_A_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
U133_X3P:204219_3P_S_AT proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
HU35KSUBA:AA348686_AT Proteasome (prosome, macropain) 26S subunit, ATPase, 1 hs
MU11KSUBA:U39302_S_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MU11KSUBB:MSA.33585.0_F_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MG-U74AV2:160152_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOE430A:1439392_X_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOUSE430A_2:1439392_X_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOUSE430_2:1439392_X_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOE430A:1416005_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOUSE430A_2:1416005_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MOUSE430_2:1416005_AT protease (prosome, macropain) 26S subunit, ATPase 1 mm
MU11KSUBB:MSA.29005.0_S_AT Protease (prosome, macropain) 26S subunit, ATPase 1, mRNA (cDNA clone MGC:6597 IMAGE:3486436) mm
MU19KSUBC:TC35203_S_AT Protease (prosome, macropain) 26S subunit, ATPase 1, mRNA (cDNA clone MGC:6597 IMAGE:3486436) mm
RG-U34A:D50696_AT peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
RAE230A:1398792_AT peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
RAT230_2:1398792_AT peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
RAE230B:1381035_AT Peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
RAT230_2:1381035_AT Peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
RG-U34B:RC_AI045861_AT Peptidase (prosome, macropain) 26S subunit, ATPase 1 rn
YEAST_2:1773979_AT One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30163 protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65906 Proteasome regulatory particle, atpase-like protein 2 [Caenorhabditis elegans] ref|NP_504558.1| F29G9.5 [Caenorhabditis elegans] sp|O16368|PRS4_CAEEL Probable 26S protease regulatory subunit 0.0
blast CAE64528 Hypothetical protein CBG09267 [Caenorhabditis briggsae] 0.0
blast AAF56205 CG5289-PA [Drosophila melanogaster] ref|NP_524469.2| CG5289-PA [Drosophila melanogaster] gb|AAL13988.1| SD02658p [Drosophila melanogaster] sp|P48601|PRS4_DROME 26S protease regulatory subunit 4 (P26s4) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 1.6E-86
Sequence
Target Sequence 
>C. ELEGANS:190174_S_AT
aacgctacttgccaaggctgttgctaatcagacgtcagctactttccttcgtattgttgg
ttcggaacttattcagaaatacctcggagatggtccaaagatggtccgtgagctcttccg
cgttgctgaggagaatgcaccatcaattgtgttcattgatgaaattgatgctgttggaac
aaaacgttacgattcgaactctggtggagaacgtgaaattcagcggactatgcttgaact
tctcaatcaacttgatggattcgattcccgtggagatgttaaagttttgatggccactaa
ccgcatcgaatctctcgaccctgctctcattcgtccaggtcgtattgatcgtaagattga
attccccctgccagatgaaaaaacgaagcgccgtatcttccagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGCTACTTGCCAAGGCTGTTGCT 478 143 717 Antisense
TAATCAGACGTCAGCTACTTTCCTT 630 635 741 Antisense
GCTACTTTCCTTCGTATTGTTGGTT 156 303 754 Antisense
GCTCTTCCGCGTTGCTGAGGAGAAT 263 301 828 Antisense
GTTACGATTCGAACTCTGGTGGAGA 619 445 902 Antisense
AGCGGACTATGCTTGAACTTCTCAA 643 81 938 Antisense
ACTTGATGGATTCGATTCCCGTGGA 374 103 966 Antisense
AAGTTTTGATGGCCACTAACCGCAT 631 155 998 Antisense
CTCTCATTCGTCCAGGTCGTATTGA 643 225 1040 Antisense
TTGAATTCCCCCTGCCAGATGAAAA 692 683 1073 Antisense
AAACGAAGCGCCGTATCTTCCAGAT 551 125 1097 Antisense
Sequence Source Affymetrix Proprietary Database