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[190170_s_at] zinc metalloprotease

Description W09D12.1 /REP_DB=WormBase Gene ID /WP=CE20173 /TR=Q23227 /GB=CAB01669.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 88      6grp_total > 87      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2175
1 2 3 4 range
A 824.5
(-2.6)
P 		816.2
(-2.7)
P 		742.9
(-3)
P 		2078.9
(3)
P 		1336
B 861.4
(-2.6)
P 		744.8
(-2.9)
P 		724.6
(-3)
P 		2899.2
(3)
P 		2175
C 847.6
(-2.8)
P 		803.7
(-3)
P 		825.9
(-2.9)
P 		2549.9
(3)
P 		1746
avr 844.5
(-2.7) 		788.2
(-2.9) 		764.5
(-3) 		2509.3
(3) 		1745

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1332
1 2 3 4 5 6 range
A 1065.2
(-2.1)
P 		933.7
(-2.8)
P 		887
(-3)
P 		1329.8
(-0.9)
P 		1403
(-0.5)
P 		2124
(3)
P 		1237
B 1075
(-2.2)
P 		914.8
(-3)
P 		1054.7
(-2.3)
P 		1220.8
(-1.4)
P 		1365.4
(-0.6)
P 		2061.4
(3)
P 		1147
C 935.8
(-2.8)
P 		919.1
(-2.8)
P 		883.6
(-3)
P 		1324.1
(-1)
P 		1430.4
(-0.5)
P 		2215.7
(3)
P 		1332
avr 1025.3
(-2.5) 		922.5
(-3) 		941.8
(-2.9) 		1291.6
(-1.2) 		1399.6
(-0.6) 		2133.7
(3) 		1211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 450
1 2 3 4 range
A 300.2
(2.8)
P 		177.2
(-3)
P 		194.2
(-2.2)
P 		304.3
(3)
P 		127
B 274.7
(-1.6)
P 		184
(-2.8)
P 		167.9
(-3)
P 		617.8
(3)
P 		450
C 280.6
(-1.5)
P 		231.1
(-3)
P 		287.9
(-1.3)
P 		429.9
(3)
P 		199
avr 285.2
(-0.9) 		197.4
(-3) 		216.7
(-2.5) 		450.7
(3) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 576
1 2 3 4 5 6 range
A 196.4
(-2.9)
P 		332.1
(-0.4)
P 		193.2
(-3)
P 		337.3
(-0.3)
P 		423.4
(1.3)
P 		517.1
(3)
P 		324
B 374.1
(-1.6)
P 		304.5
(-2.4)
P 		256.1
(-3)
P 		323.3
(-2.2)
P 		554.7
(0.5)
P 		768.7
(3)
P 		513
C 346.9
(-1.6)
P 		280.5
(-2.4)
P 		224.9
(-3)
P 		266.3
(-2.5)
P 		462.8
(-0.2)
P 		741.7
(3)
P 		517
avr 305.8
(-1.9) 		305.7
(-1.9) 		224.7
(-3) 		309
(-1.9) 		480.3
(0.4) 		675.8
(3) 		451
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190170_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12857
Sequence Type Exemplar sequence
Representative Public ID W09D12.1 NCBI
Target Description W09D12.1 /REP_DB=WormBase Gene ID /WP=CE20173 /TR=Q23227 /GB=CAB01669.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074277(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074277 NCBI Caenorhabditis elegans W09D12.1 (W09D12.1) mRNA, complete cds. 11/11 None
GENEFINDER00000007082 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14920396:14922007:-1 10/11 None
W09D12.1.1 ENSEMBL cdna:known chromosome:CEL140:V:14920285:14924198:-1 gene:W09D12.1 11/11 None
W09D12.1.2 ENSEMBL cdna:known chromosome:CEL140:V:14920338:14924198:-1 gene:W09D12.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000007077 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14920395-14926139(-) 98.7 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Ensembl W09D12.1
Entrez Gene 179991 Entrez gene
SwissProt Q23227 EMBL-EBI
WormBase CE33197 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5578 extracellular matrix (sensu Metazoa) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01669 Hypothetical protein W09D12.1 [Caenorhabditis elegans] ref|NP_506678.2| W09D12.1 [Caenorhabditis elegans] 0.0
blast CAB01669 Hypothetical protein W09D12.1 [Caenorhabditis elegans] ref|NP_506678.2| W09D12.1 [Caenorhabditis elegans] 1.0E-117
blast AAR11447 matrix metalloproteinase [Globodera rostochiensis] 4.0E-53
blast AAR11446 matrix metalloproteinase [Heterodera glycines] gb|AAR11445.1| matrix metalloproteinase [Heterodera glycines] 2.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 5.5E-4
Sequence
Target Sequence 
>C. ELEGANS:190170_S_AT
atggactttcatccgtcgatcattcaactccgtcgtcagatgttgatcgttcaaaatgcc
caaagcatcttgactctgttgttgctataaacgaccaagaatggctcttcttccgttcca
acaaagtgtacaaactcaacaatcgaaagttcgttgactcgggtcgcccaattcaacaag
tgttcccacgtggcccacaatttgtcaacgccactgtgtcatctggaccattgactcttt
tgttcgtggaacgaactatttatggatatgaatacgatggagtgcagtttacggaagctc
caaattatccaaaagaacttcacgatcgtgttctcttctacccacagggagcattcccac
tgaataatgggagtgtcattcttttgtcgggtaacgtgtttgctacctacaatgtgcaac
agaatgctccatcattcttgaacgacaaaaatcgatatttcccaaatttgccagaagata
tcaaaagtggaattgaaaagactcaagggttcactgatgcatattatatgtttgatgagg
ctacggtctccgactacgatatgaactca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGACTTTCATCCGTCGATCATTC 614 51 1541 Antisense
ATCATTCAACTCCGTCGTCAGATGT 416 27 1559 Antisense
ATGCCCAAAGCATCTTGACTCTGTT 631 41 1596 Antisense
CTCTTCTTCCGTTCCAACAAAGTGT 343 229 1645 Antisense
GTCGCCCAATTCAACAAGTGTTCCC 592 473 1703 Antisense
CACGTGGCCCACAATTTGTCAACGC 314 199 1727 Antisense
CTGGACCATTGACTCTTTTGTTCGT 427 241 1763 Antisense
TACCCACAGGGAGCATTCCCACTGA 390 647 1879 Antisense
GAGTGTCATTCTTTTGTCGGGTAAC 232 401 1911 Antisense
GTTTGATGAGGCTACGGTCTCCGAC 704 451 2070 Antisense
GGTCTCCGACTACGATATGAACTCA 396 501 2085 Antisense
Sequence Source Affymetrix Proprietary Database