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[190164_s_at] DNA-binding protein

Description F38A5.13 /REP_DB=WormBase Gene ID /WP=CE10050 /TR=Q94216 /GB=AAB09157.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=DNA-binding protein
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 56      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 73      6grp_total > 47      4grp_total > 62      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 377
1 2 3 4 range
A 738.6
(3)
P 		666.2
(0.2)
P 		583.6
(-3)
P 		595
(-2.6)
P 		155
B 777.6
(3)
P 		499.3
(-1.4)
P 		688.9
(1.6)
P 		400.8
(-3)
P 		377
C 702.4
(3)
P 		619.4
(1.2)
P 		615.4
(1.1)
P 		431.8
(-3)
P 		271
avr 739.5
(3) 		595
(-0.3) 		629.3
(0.5) 		475.9
(-3) 		264

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 308
1 2 3 4 5 6 range
A 554.2
(-1)
P 		571.1
(0.3)
P 		551.2
(-1.3)
P 		567.8
(0)
P 		529
(-3)
P 		605.4
(3)
P 		76
B 492.1
(-2.1)
P 		589.6
(-0.2)
P 		446.1
(-3)
P 		582.8
(-0.3)
P 		482.5
(-2.3)
P 		754.2
(3)
P 		308
C 721.2
(3)
P 		641.5
(1.1)
P 		489.7
(-2.6)
P 		472.4
(-3)
P 		487.3
(-2.6)
P 		566.1
(-0.7)
P 		249
avr 589.2
(0.8) 		600.7
(1.3) 		495.7
(-3) 		541
(-1.1) 		499.6
(-2.8) 		641.9
(3) 		146
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 268.9
(3)
P 		144.6
(-1.1)
P 		152.6
(-0.8)
P 		87.1
(-3)
P 		182
B 248
(3)
P 		123.3
(-1.6)
P 		159.6
(-0.3)
P 		85.4
(-3)
P 		163
C 232.5
(3)
P 		178.1
(1)
P 		214.5
(2.3)
P 		72.8
(-3)
P 		160
avr 249.8
(3) 		148.7
(-0.6) 		175.6
(0.4) 		81.8
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 102.2
(-3)
P 		203.1
(3)
P 		120
(-1.9)
P 		144
(-0.5)
P 		159.6
(0.4)
P 		147.4
(-0.3)
P 		101
B 171.2
(-0.8)
P 		196.3
(0.1)
P 		108.3
(-3)
P 		154.4
(-1.4)
P 		196
(0)
P 		281.2
(3)
P 		173
C 267.3
(3)
P 		195.8
(0.5)
P 		124.6
(-2)
P 		95
(-3)
P 		157.7
(-0.8)
P 		189.5
(0.3)
P 		172
avr 180.2
(1.2) 		198.4
(2.5) 		117.6
(-3) 		131.1
(-2.1) 		171.1
(0.6) 		206
(3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190164_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10656
Sequence Type Exemplar sequence
Representative Public ID F38A5.13 NCBI
Target Description F38A5.13 /REP_DB=WormBase Gene ID /WP=CE10050 /TR=Q94216 /GB=AAB09157.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=DNA-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068605 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-11) (dnj-11) mRNA, complete cds. 11/11 None
SNAP00000010564 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6585855:6587881:1 11/11 None
GENEFINDER00000010580 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6585855:6587881:1 11/11 None
F38A5.13 ENSEMBL cdna:known chromosome:CEL140:IV:6585855:6588153:1 gene:F38A5.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6585860-6587887(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNA-binding protein
Gene Symbol dnj-11
Ensembl F38A5.13
Entrez Gene 177419 Entrez gene
SwissProt Q94216 EMBL-EBI
WormBase CE10050 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259284_AT DNAJ heat shock N-terminal domain-containing protein / cell division protein-related at
CANINE:1589905_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 2 isoform 2 cfa
CANINE_2:CFA.10112.1.A1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 2 isoform 2 cfa
CANINE_2:CFA.21020.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 2 isoform 2 cfa
DROSOPHILA_2:1623998_AT dm
DROSGENOME1:154210_AT dm
CHICKEN:GGA.11919.1.S1_S_AT similar to zuotin related factor 1 gga
MOUSE430A_2:1454219_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430_2:1454219_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOE430A:1454219_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430A_2:1425910_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOE430A:1425910_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430_2:1425910_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MU11KSUBA:U53208_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MG-U74AV2:104738_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MG-U74AV2:101890_F_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MG-U74CV2:171207_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOE430A:1417657_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430A_2:1417657_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430_2:1417657_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOE430A:1448794_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430A_2:1448794_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
MOUSE430_2:1448794_S_AT DnaJ (Hsp40) homolog, subfamily C, member 2 mm
RG-U34B:RC_AA964764_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
RAE230A:1373004_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
RAT230_2:1373004_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
RAE230B:1391487_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
RAT230_2:1391487_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
RG-U34B:RC_AI044807_AT DnaJ (Hsp40) homolog, subfamily C, member 2 rn
YEAST_2:1778611_AT Cytosolic ribosome-associated chaperone, contains a DnaJ domain; together with Ssz1p, acts as a chaperone for nascent polypeptide chains Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB09157 Dnaj domain (prokaryotic heat shock protein) protein 11 [Caenorhabditis elegans] ref|NP_501006.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-11) [Caenorhabditis elegans] 0.0
blast CAE61509 Hypothetical protein CBG05408 [Caenorhabditis briggsae] 0.0
blast XP_396658 PREDICTED: similar to CG10565-PA [Apis mellifera] 9.0E-86
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.0000000116861E-7
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.9E-16
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 0.0047
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 7.7E-10
Sequence
Target Sequence 
>C. ELEGANS:190164_S_AT
gagcggatttgcattgggttcactgttgatcaactcagagagctgtgcgagaaggtcgaa
tcactttcaattgcttctgaaattcaaactgcgctaacagatgctgaaattctgaagaaa
gaagctgctggagcaaaggtcacaatcactgaggataagaacaaagaaaatgaaaagcaa
gccgacaaggagacatggactagtgaagagattcaattattggttaaggcttcaaacaca
ttcccacctggaaccgtggagagatgggttcaaattgccgattacattaacgagcatcgc
aaggattctacgggtttaccaccaaaaactgaaaaacaggttatcaagcaatgcaaagct
gttcaaacgatgaacgtcaagctaccatcaactactcaaaatcagctaggaactgcgcta
ccagatgaggatgtctggtcagcaaccgaacagaaaactttagaggacgctatcaagaag
cataagtcaagtgatcccgagcggtgggaaaagatctcaacagaagttggcacaaagtcc
aaaaaagcctgcattcgtcgtttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGGATTTGCATTGGGTTCACTG 143 385 1183 Antisense
GGTCGAATCACTTTCAATTGCTTCT 617 501 1236 Antisense
GCTGCTGGAGCAAAGGTCACAATCA 301 297 1306 Antisense
TTAAGGCTTCAAACACATTCCCACC 163 683 1406 Antisense
CATTCCCACCTGGAACCGTGGAGAG 43 217 1421 Antisense
GATGGGTTCAAATTGCCGATTACAT 69 409 1445 Antisense
GAGCATCGCAAGGATTCTACGGGTT 47 389 1474 Antisense
CAAGCTACCATCAACTACTCAAAAT 444 185 1560 Antisense
ATCAGCTAGGAACTGCGCTACCAGA 243 29 1583 Antisense
TAAGTCAAGTGATCCCGAGCGGTGG 515 633 1665 Antisense
AAAAAAGCCTGCATTCGTCGTTTCA 464 127 1723 Antisense
Sequence Source Affymetrix Proprietary Database