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[190161_at] Na/Ca,K antiporter

Description C07A9.4 /REP_DB=WormBase Gene ID /WP=CE00500 /TR=SW:P34315 /GB=CAA82339.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Na-Ca,K antiporter
Cluster 6grp_high1_10_10 > 14      4grp_high_8_8 > 18      6grp_total > 03      4grp_total > 46      6grp_cgc > 40      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 131.3
(3)
P 		60.4
(-2.9)
M 		58.6
(-3)
P 		62.4
(-2.7)
A 		73
B 143.8
(3)
P 		89.5
(-0)
P 		81.3
(-0.5)
P 		35.4
(-3)
A 		108
C 115.1
(3)
P 		102.5
(1.3)
P 		71.7
(-3)
P 		111.7
(2.5)
P 		43
avr 130.1
(3) 		84.1
(-1.6) 		70.5
(-2.9) 		69.8
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 47.5
(1.5)
A 		46.1
(1.2)
P 		44.4
(0.9)
P 		43
(0.6)
P 		55.2
(3)
P 		24.5
(-3)
A 		31
B 48.7
(-1)
P 		84.1
(2.5)
P 		47.9
(-1.1)
P 		89
(3)
P 		28.9
(-3)
P 		41.3
(-1.8)
P 		60
C 86.8
(3)
P 		73.6
(1.6)
P 		32.1
(-3)
A 		75.3
(1.7)
A 		47.6
(-1.3)
P 		46
(-1.5)
P 		55
avr 61
(1.5) 		67.9
(2.8) 		41.5
(-2.2) 		69.1
(3) 		43.9
(-1.8) 		37.3
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 47.8
(3)
P 		13.1
(-2.4)
M 		15.3
(-2)
P 		9.1
(-3)
A 		39
B 45.9
(3)
P 		22.1
(-0.7)
P 		18.8
(-1.2)
P 		7.6
(-3)
A 		38
C 38.1
(3)
P 		29.5
(0.3)
P 		25
(-1.1)
P 		18.8
(-3)
P 		19
avr 43.9
(3) 		21.6
(-1.2) 		19.7
(-1.5) 		11.8
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 8.8
(-1.4)
A 		16.4
(2.8)
P 		9.7
(-0.9)
P 		10.9
(-0.3)
P 		16.7
(3)
P 		6
(-3)
A 		11
B 17
(-1)
P 		28
(3)
P 		11.6
(-3)
P 		23.6
(1.4)
P 		11.7
(-3)
P 		15.4
(-1.6)
P 		16
C 32.2
(3)
P 		22.5
(0.6)
P 		8.2
(-3)
A 		15.2
(-1.3)
A 		15.4
(-1.2)
P 		15.4
(-1.2)
P 		24
avr 19.3
(1.6) 		22.3
(3) 		9.8
(-3) 		16.6
(0.3) 		14.6
(-0.7) 		12.3
(-1.8) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190161_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6969
Sequence Type Exemplar sequence
Representative Public ID C07A9.4 NCBI
Target Description C07A9.4 /REP_DB=WormBase Gene ID /WP=CE00500 /TR=SW:P34315 /GB=CAA82339.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Na-Ca,K antiporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066745(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066745 NCBI Caenorhabditis elegans Na/Ca eXchangers family member (ncx-6) (ncx-6) mRNA, complete cds. 10/11 None
GENEFINDER00000039343 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9710208:9713824:-1 10/11 None
C07A9.4 ENSEMBL cdna:known chromosome:CEL140:III:9710208:9713824:-1 gene:C07A9.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039332 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9710225-9713820(-) 98.59 100.0
Public Domain and Genome References
Gene Title Na/Ca,K antiporter
Gene Symbol ncx-6
Ensembl C07A9.4
Entrez Gene 182354 Entrez gene
SwissProt P34315 EMBL-EBI
WormBase CE33595 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82339 Hypothetical protein C07A9.4 [Caenorhabditis elegans] ref|NP_499146.2| Na/Ca eXchangers family member (ncx-6) [Caenorhabditis elegans] sp|P34315|NCX6_CAEEL Putative sodium/calcium exchanger 6 precursor (Na(+)/Ca(2+)-exchange protein 6) 0.0
blast S40705 Na+/Ca2+,K+-exchanging protein homolog - Caenorhabditis elegans 0.0
blast CAE65149 Hypothetical protein CBG10015 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 2.5E-27
Pfam IPR004837 EMBL-EBI Sodium/calcium exchanger membrane region 2.8E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499146.2 11 92-114,142-164,171-193,208-225,230-252,369-391,398-420,430-452,498-520,535-554,566-588
C07A9.4 11 92-114,142-164,171-193,208-225,230-252,369-391,398-420,430-452,498-520,535-554,566-588
GENEFINDER00000039343 11 92-114,142-164,171-193,208-225,230-252,369-391,398-420,430-452,498-520,535-554,566-588
Sequence
Target Sequence 
>C. ELEGANS:190161_AT
tcttttacagtgcctcatttgtccggttttccttcttttctgtattcaaggtatttccat
ttttaattcaatattttccaactacagtttctacagtttgttcaatatctccatttccaa
attctcctggtctctggatagtttaatatcctatgcaggatttctaatgtccattgcatg
gatttatttaatttcttcggaagttgtaaatgttgtaacaatgcttggagttgtttcaag
agtatcacatgaagtacttggtctaacaattcttgcatggagtaatagtattggagactt
gattgctgatgtttcagttgcaaagcaaggttatcccagaatggcaatggctgcagcaat
tggtggacagttgtttagtatatctcctaattggatttggtcttccattcacaattgcca
agattcaaggaaaatctatatcgatgattataaatccaacttatcgacttttaatgcttt
tccttgcaatttctttgattttcactttaattgcaatgtttgcacagaaatactttttac
gacgtattcactcatattctcttgtcttcatctacatttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTTTACAGTGCCTCATTTGTCCG 485 615 1179 Antisense
GTTTTCCTTCTTTTCTGTATTCAAG 145 449 1204 Antisense
TTCAATATTTTCCAACTACAGTTTC 350 685 1245 Antisense
GTTTGTTCAATATCTCCATTTCCAA 571 451 1274 Antisense
CATTTCCAAATTCTCCTGGTCTCTG 130 215 1290 Antisense
GCAGGATTTCTAATGTCCATTGCAT 422 311 1333 Antisense
ATCCCAGAATGGCAATGGCTGCAGC 532 37 1511 Antisense
TGGTCTTCCATTCACAATTGCCAAG 136 553 1576 Antisense
ATCGACTTTTAATGCTTTTCCTTGC 4 35 1641 Antisense
GACGTATTCACTCATATTCTCTTGT 117 379 1719 Antisense
ATTCTCTTGTCTTCATCTACATTTC 305 11 1734 Antisense
Sequence Source Affymetrix Proprietary Database