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[190154_at]

Description F44F1.1 /REP_DB=WormBase Gene ID /WP=CE10412 /TR=O02259 /GB=CAB03099.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain-like protease
Cluster 6grp_total > 46      4grp_total > 34      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 6.3
(-3)
A 		29.2
(0.9)
A 		35.5
(1.9)
A 		41.7
(3)
A 		35
B 5.4
(-3)
A 		52.3
(3)
A 		31
(0.3)
A 		16.9
(-1.5)
A 		47
C 34.2
(0.5)
A 		33.3
(0.3)
A 		10.4
(-3)
A 		51.6
(3)
P 		41
avr 15.3
(-3) 		38.3
(3) 		25.6
(-0.3) 		36.7
(2.6) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 52.5
(3)
A 		35
(-2.1)
A 		32.1
(-3)
A 		44.6
(0.7)
A 		41.5
(-0.2)
A 		40.9
(-0.4)
A 		20
B 14.5
(-1.6)
A 		28.9
(1.9)
A 		29.3
(2)
A 		31.2
(2.4)
A 		8.7
(-3)
A 		33.6
(3)
A 		25
C 21.4
(-0.6)
A 		50.3
(3)
A 		6.8
(-2.4)
A 		17.3
(-1.1)
A 		2.3
(-3)
A 		34.8
(1.1)
A 		48
avr 29.5
(0.5) 		38.1
(3) 		22.7
(-1.5) 		31
(0.9) 		17.5
(-3) 		36.4
(2.5) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 2.3
(-3)
A 		6.3
(0.4)
A 		9.3
(3)
A 		6.1
(0.3)
A 		7
B 1.7
(-3)
A 		12.9
(3)
A 		7.2
(-0.1)
A 		3.6
(-2)
A 		11
C 11.3
(3)
A 		9.6
(1.7)
A 		3.6
(-3)
A 		8.7
(1)
P 		8
avr 5.1
(-3) 		9.6
(3) 		6.7
(-0.9) 		6.1
(-1.7) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 9.7
(-0.1)
A 		12.4
(2.9)
A 		7
(-3)
A 		11.3
(1.7)
A 		12.5
(3)
A 		10
(0.3)
A 		6
B 5
(-2)
A 		9.6
(1.1)
A 		7.1
(-0.6)
A 		8.3
(0.2)
A 		3.5
(-3)
A 		12.5
(3)
A 		9
C 7.9
(-0)
A 		15.3
(3)
A 		1.7
(-2.6)
A 		3.5
(-1.8)
A 		0.7
(-3)
A 		11.6
(1.5)
A 		15
avr 7.5
(-1.1) 		12.4
(3) 		5.3
(-3) 		7.7
(-1) 		5.6
(-2.7) 		11.4
(2.2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190154_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2020
Sequence Type Exemplar sequence
Representative Public ID F44F1.1 NCBI
Target Description F44F1.1 /REP_DB=WormBase Gene ID /WP=CE10412 /TR=O02259 /GB=CAB03099.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=calpain-like protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060924(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060924 NCBI Caenorhabditis elegans F44F1.1 (F44F1.1) mRNA, complete cds. 11/11 None
SNAP00000015368 ENSEMBL cdna:SNAP chromosome:CEL140:I:13258638:13261021:1 11/11 None
GENEFINDER00000015379 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13258638:13261021:1 11/11 None
F44F1.1 ENSEMBL cdna:known chromosome:CEL140:I:13258662:13261021:1 gene:F44F1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13258568-13260952(+) 98.27 100.0
Public Domain and Genome References
Ensembl F44F1.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493325 F44F1.1 [Caenorhabditis elegans] 0.0
blast CAB03101 Hypothetical protein F44F1.3 [Caenorhabditis elegans] ref|NP_493327.2| F44F1.3 [Caenorhabditis elegans] 0.0
blast CAE72273 Hypothetical protein CBG19393 [Caenorhabditis briggsae] 1.0E-45
blast CAE72273 Hypothetical protein CBG19393 [Caenorhabditis briggsae] 1.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.2E-5
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 4.5E-7
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.2E-5
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 4.5E-7
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.2E-5
Sequence
Target Sequence 
>C. ELEGANS:190154_AT
ggggatattgatttggaacctggtagctacgttattacagtgaataaactgaacaactcg
cccataggcaaacgagatatctcaattcactcttcccgaccaatttcggtcaatgaaggg
tttttggtaacaagtgaagatggtgaacatataaaatatgtagtggataagtttggacag
gaaattgtgaaagagccgaaggataaaatgagcattaaaaagtacactgataataattcc
acatttatcgctgtagttgcatggaacttcgcatatgatcagtttctacatgttcatttg
cgttactcaatcaccgatgaacaattggtctctagaagtttagaagacaatcaaacagtt
gatgttattcctccaaggggtgttcagattttggtagtaatcgaacaatatgcaatgccg
gagattgtggatttcccaattgacattgattataaactttcaaaagatgttgattcaaca
atcaatggaatcaaaagaggatgtcatcttccagaaatatcttcatctcgattcttagag
caagttcaccgtgaggagcgaatttccgtggctgactgttttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGGATATTGATTTGGAACCTGGTA 706 495 1252 Antisense
GGAACCTGGTAGCTACGTTATTACA 682 529 1266 Antisense
ATAAACTGAACAACTCGCCCATAGG 385 25 1295 Antisense
CGCCCATAGGCAAACGAGATATCTC 43 257 1310 Antisense
ATTCCACATTTATCGCTGTAGTTGC 367 7 1487 Antisense
AGTTGATGTTATTCCTCCAAGGGGT 416 63 1608 Antisense
ATTCCTCCAAGGGGTGTTCAGATTT 484 7 1618 Antisense
ATGCCGGAGATTGTGGATTTCCCAA 445 39 1666 Antisense
GATTCTTAGAGCAAGTTCACCGTGA 264 431 1781 Antisense
GTTCACCGTGAGGAGCGAATTTCCG 132 445 1795 Antisense
GAATTTCCGTGGCTGACTGTTTTCT 601 325 1811 Antisense
Sequence Source Affymetrix Proprietary Database