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[190151_s_at] calcium-binding protein

Description C33D12.4 /REP_DB=WormBase Gene ID /WP=CE06906 /CHR=X /FEA=Sanger Annotation /DEF=ras-related protein (ST.LOUIS) TR:Q22906 protein_id:AAB04568.1
Cluster 6grp_total > 54      4grp_total > 60      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 68.7
(3)
A 		48.2
(0.1)
A 		57.5
(1.4)
A 		27
(-3)
A 		42
B 75.3
(2.3)
A 		82.7
(3)
A 		64.4
(1.3)
A 		17.2
(-3)
A 		66
C 65.6
(-1.5)
A 		75.7
(2.5)
A 		77
(3)
A 		61.7
(-3)
A 		15
avr 69.9
(3) 		68.9
(2.8) 		66.3
(2.4) 		35.3
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 8.1
(-3)
A 		9.7
(-2.9)
A 		51.2
(1)
A 		26.9
(-1.3)
A 		73.5
(3)
A 		58.2
(1.6)
A 		65
B 45.1
(1.5)
A 		48.2
(2)
A 		53.9
(3)
A 		19.4
(-3)
A 		47.9
(2)
A 		27.3
(-1.6)
A 		35
C 62
(3)
A 		37.2
(-1.6)
A 		61.7
(2.9)
A 		47.7
(0.3)
A 		29.9
(-3)
A 		55
(1.7)
A 		32
avr 38.4
(-1.2) 		31.7
(-2.9) 		55.6
(3) 		31.3
(-3) 		50.4
(1.7) 		46.8
(0.8) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 25
(3)
A 		10.5
(-1.1)
A 		15
(0.1)
A 		4
(-3)
A 		21
B 24
(3)
A 		20.4
(1.9)
A 		14.9
(0.3)
A 		3.7
(-3)
A 		20
C 21.7
(1.1)
A 		21.8
(1.2)
A 		26.8
(3)
A 		10.4
(-3)
A 		16
avr 23.6
(3) 		17.6
(1) 		18.9
(1.4) 		6
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 1.5
(-3)
A 		3.5
(-2.4)
A 		11.1
(-0.2)
A 		6.8
(-1.5)
A 		22.2
(3)
A 		14.2
(0.7)
A 		21
B 15.7
(1.4)
A 		16.1
(1.6)
A 		13.1
(0.3)
A 		5.1
(-3)
A 		19.5
(3)
A 		10.2
(-0.9)
A 		14
C 23
(3)
A 		11.3
(-2.2)
A 		15.7
(-0.3)
A 		9.6
(-3)
A 		9.7
(-3)
A 		18.4
(0.9)
A 		13
avr 13.4
(0.8) 		10.3
(-1.1) 		13.3
(0.7) 		7.2
(-3) 		17.1
(3) 		14.3
(1.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190151_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18515
Sequence Type Exemplar sequence
Representative Public ID C33D12.4 NCBI
Target Description C33D12.4 /REP_DB=WormBase Gene ID /WP=CE06906 /CHR=X /FEA=Sanger Annotation /DEF=ras-related protein (ST.LOUIS) TR:Q22906 protein_id:AAB04568.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076122(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076122 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-312) (tag-312) mRNA, complete cds. 11/11 A 
SNAP00000019206 ENSEMBL cdna:SNAP chromosome:CEL140:X:3055412:3059376:-1 11/11 None
GENEFINDER00000019213 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3055412:3059376:-1 11/11 None
C33D12.6 ENSEMBL cdna:known chromosome:CEL140:X:3055248:3059376:-1 gene:C33D12.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3055411-3056093(-) 100.0 100.0
Public Domain and Genome References
Gene Title calcium-binding protein
Gene Symbol tag-312
Ensembl C33D12.6
Entrez Gene 180593 Entrez gene
SwissProt Q22908 EMBL-EBI
WormBase CE27102 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.3084.1.S1_AT similar to RAS and EF hand domain containing cfa
HU35KSUBD:RC_R78618_AT RAS and EF-hand domain containing hs
U133_X3P:HS2.351442.2.S1_3P_AT RAS and EF-hand domain containing hs
U133_X3P:HS2.351442.1.S1_3P_X_AT RAS and EF-hand domain containing hs
U133_X3P:HS2.351442.1.S1_3P_AT RAS and EF-hand domain containing hs
HG-U133_PLUS_2:1553185_AT RAS and EF-hand domain containing hs
HG-U133_PLUS_2:1553186_X_AT RAS and EF-hand domain containing hs
HG-U133_PLUS_2:1553986_AT RAS and EF-hand domain containing hs
HG-U95B:44625_AT RAS and EF-hand domain containing hs
HG-U95B:44626_G_AT RAS and EF-hand domain containing hs
HU35KSUBD:RC_AA621523_AT RAS and EF-hand domain containing hs
U133_X3P:HS.271039.0.A1_3P_AT RAS and EF-hand domain containing hs
U133_X3P:HS.165464.0.A1_3P_X_AT RAS and EF-hand domain containing hs
U133_X3P:HS.165464.0.A1_3P_AT RAS and EF-hand domain containing hs
HG-U133_PLUS_2:235144_AT RAS and EF-hand domain containing hs
HG-U133B:235144_AT RAS and EF-hand domain containing hs
HG-U133_PLUS_2:243862_AT RAS and EF-hand domain containing hs
HG-U133B:243862_AT RAS and EF-hand domain containing hs
HG-U95B:59406_AT RAS and EF-hand domain containing hs
HG-U95D:84013_AT RAS and EF-hand domain containing hs
HG-U95D:77541_AT RAS and EF-hand domain containing hs
MOE430B:1458591_AT RAS and EF hand domain containing mm
MOUSE430_2:1458591_AT RAS and EF hand domain containing mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39204 Hypothetical protein C33D12.6 [Caenorhabditis elegans] ref|NP_508523.1| Temporarily Assigned Gene name family member (tag-312) [Caenorhabditis elegans] 0.0
blast CAE68369 Hypothetical protein CBG14124 [Caenorhabditis briggsae] 0.0
blast T25608 hypothetical protein C33D12.5 - Caenorhabditis elegans 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.017
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.8E-6
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.6E-65
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.6E-65
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.6E-65
Sequence
Target Sequence 
>C. ELEGANS:190151_S_AT
tgtgtggagacgccgctgttggaaaaagtagttttgtaatgcgggtgatccgtcgtcaat
tcacaaatcagctccccagcacacttggcgtagattttcacgtgaagactgtcaatgttg
acggaagaaacgttgcacttcagctatgggacaccgcgggacaagaacgtttccgatcac
tttgtaaatcatattttcgtcgagctgatggagcaatcctagtctatgacgtctgtgcgg
agcagtcgtttctccgtgttcgcgactggattgagactataaaggagtcaacagaaagga
gtattccgatcattttggtaggaaacaaggttgatatgcgtatttcgacgccaggctcgg
tggcaaagacagatggtgcaagtatggctgcggcgatgggagttctattcatggaaacca
gtgctttagacggaagtaacattgataacgctatgttggcactaaccagagagctcatgg
cagtagaagatgttgaaattcgctcaacaggtgttgtgctcaatccagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGTGGAGACGCCGCTGTTGGAAA 234 549 14 Antisense
AATGCGGGTGATCCGTCGTCAATTC 56 167 51 Antisense
TCCCCAGCACACTTGGCGTAGATTT 388 595 86 Antisense
ACGTTGCACTTCAGCTATGGGACAC 47 95 143 Antisense
GAACGTTTCCGATCACTTTGTAAAT 386 345 178 Antisense
GAGCAATCCTAGTCTATGACGTCTG 659 389 224 Antisense
ACGTCTGTGCGGAGCAGTCGTTTCT 302 95 242 Antisense
TTCTCCGTGTTCGCGACTGGATTGA 619 693 263 Antisense
TTTCGACGCCAGGCTCGGTGGCAAA 480 669 356 Antisense
GTATGGCTGCGGCGATGGGAGTTCT 160 457 395 Antisense
CAACAGGTGTTGTGCTCAATCCAGC 608 187 518 Antisense
Sequence Source Affymetrix Proprietary Database