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[190145_s_at]

Description F32D8.4 /REP_DB=WormBase Gene ID /WP=CE23698 /TR=Q19965 /GB=CAA98456.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=lactate dehydrogenase
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 56      6grp_total > 64      4grp_total > 58      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 993
1 2 3 4 range
A 1013.6
(-0.1)
P 		1436.3
(3)
P 		1269.2
(1.8)
P 		613
(-3)
P 		823
B 1022
(0.1)
P 		1398
(2.4)
P 		1495.4
(3)
P 		502.4
(-3)
P 		993
C 965.1
(-0.8)
P 		1423.5
(3)
P 		1426.2
(3)
P 		700.1
(-3)
P 		726
avr 1000.2
(-0.1) 		1419.3
(3) 		1396.9
(2.8) 		605.2
(-3) 		814

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 942
1 2 3 4 5 6 range
A 2146.6
(0.1)
P 		1944.4
(-1.2)
P 		2513
(2.4)
P 		2600.9
(3)
P 		1659.4
(-3)
P 		1973.9
(-1)
P 		942
B 1965.8
(-0.9)
P 		1901.2
(-1.8)
P 		2232.9
(3)
P 		1821.4
(-3)
P 		1950.7
(-1.1)
P 		2216.5
(2.8)
P 		412
C 1892
(-1.8)
P 		1861.4
(-2)
P 		2317.9
(1.5)
P 		2242.8
(0.9)
P 		1736.8
(-3)
P 		2508.2
(3)
P 		771
avr 2001.5
(-0.7) 		1902.3
(-1.7) 		2354.6
(3) 		2221.7
(1.6) 		1782.3
(-3) 		2232.9
(1.7) 		572
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 407
1 2 3 4 range
A 369
(3)
P 		311.8
(1.8)
P 		331.8
(2.2)
P 		89.7
(-3)
P 		279
B 325.9
(2.5)
P 		345.3
(3)
P 		346.5
(3)
P 		107.1
(-3)
P 		239
C 319.5
(0.2)
P 		409.3
(1.6)
P 		497.1
(3)
P 		118
(-3)
P 		379
avr 338.1
(1.9) 		355.5
(2.2) 		391.8
(3) 		104.9
(-3) 		287

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 444
1 2 3 4 5 6 range
A 395.7
(-3)
P 		691.5
(3)
P 		547.3
(0.1)
P 		659.7
(2.4)
P 		500.7
(-0.9)
P 		480.6
(-1.3)
P 		296
B 684
(0.5)
P 		632.8
(-0.4)
P 		542.2
(-2)
P 		482.4
(-3)
P 		792.6
(2.4)
P 		826.5
(3)
P 		344
C 701.3
(0.9)
P 		568
(-1.2)
P 		589.9
(-0.9)
P 		451
(-3)
P 		562
(-1.3)
P 		839.6
(3)
P 		389
avr 593.7
(-1) 		630.8
(0.2) 		559.8
(-2.1) 		531
(-3) 		618.4
(-0.2) 		715.6
(3) 		185
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190145_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14653
Sequence Type Exemplar sequence
Representative Public ID F32D8.4 NCBI
Target Description F32D8.4 /REP_DB=WormBase Gene ID /WP=CE23698 /TR=Q19965 /GB=CAA98456.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=lactate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028702(11), NM_001028703(11), NM_001028701(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028702 NCBI Caenorhabditis elegans F32D8.12b (F32D8.12) mRNA, complete cds. 11/11 None
NM_001028703 NCBI Caenorhabditis elegans F32D8.12c (F32D8.12) mRNA, complete cds. 11/11 None
NM_001028701 NCBI Caenorhabditis elegans F32D8.12a (F32D8.12) mRNA, complete cds. 11/11 None
SNAP00000041027 ENSEMBL cdna:SNAP chromosome:CEL140:V:10891350:10893580:-1 11/11 None
GENEFINDER00000041044 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10891350:10893580:-1 11/11 None
F32D8.12b.3 ENSEMBL cdna:known chromosome:CEL140:V:10890460:10893580:-1 gene:F32D8.12 11/11 None
F32D8.12b.4 ENSEMBL cdna:known chromosome:CEL140:V:10890475:10893580:-1 gene:F32D8.12 11/11 None
F32D8.12c.2 ENSEMBL cdna:known chromosome:CEL140:V:10890487:10893583:-1 gene:F32D8.12 11/11 None
F32D8.12c.3 ENSEMBL cdna:known chromosome:CEL140:V:10890487:10893393:-1 gene:F32D8.12 11/11 None
F32D8.12c.4 ENSEMBL cdna:known chromosome:CEL140:V:10890487:10893580:-1 gene:F32D8.12 11/11 None
F32D8.12c.1 ENSEMBL cdna:known chromosome:CEL140:V:10890487:10893614:-1 gene:F32D8.12 11/11 None
F32D8.12b.1 ENSEMBL cdna:known chromosome:CEL140:V:10890533:10893583:-1 gene:F32D8.12 11/11 A 
F32D8.12b.2 ENSEMBL cdna:known chromosome:CEL140:V:10890533:10893593:-1 gene:F32D8.12 11/11 A 
F32D8.12a ENSEMBL cdna:known chromosome:CEL140:V:10891350:10893580:-1 gene:F32D8.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10891349-10897287(-) 100.0 100.0
Public Domain and Genome References
Ensembl F32D8.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE54902 Hypothetical protein F32D8.12b [Caenorhabditis elegans] ref|NP_001023873.1| F32D8.12b [Caenorhabditis elegans] 0.0
blast CAD54138 Hypothetical protein F32D8.12a [Caenorhabditis elegans] ref|NP_001023872.1| F32D8.12a [Caenorhabditis elegans] 0.0
blast CAE64909 Hypothetical protein CBG09729 [Caenorhabditis briggsae] 0.0
blast CAE54903 Hypothetical protein F32D8.12c [Caenorhabditis elegans] ref|NP_001023874.1| F32D8.12c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006094 EMBL-EBI FAD linked oxidase, N-terminal 4.6E-10
Pfam IPR006094 EMBL-EBI FAD linked oxidase, N-terminal 1.2E-26
Pfam IPR004113 EMBL-EBI FAD linked oxidase, C-terminal 1.0E-85
Pfam IPR006094 EMBL-EBI FAD linked oxidase, N-terminal 1.2E-26
Pfam IPR004113 EMBL-EBI FAD linked oxidase, C-terminal 1.0E-85
Pfam IPR006094 EMBL-EBI FAD linked oxidase, N-terminal 1.2E-46
Pfam IPR004113 EMBL-EBI FAD linked oxidase, C-terminal 1.0E-85
Sequence
Target Sequence 
>C. ELEGANS:190145_S_AT
gatacagttcagattcaagcttgcaactcttacagtagcctgaaccttcgagaatcacca
actcttttcattgaatttcatggtagcaatgaaaaagaagtagctgatcaaacatctgct
gttgaggatatctgtaaaagtcacgaagctctagattttgattctggagcctcacctgat
aagagggcaactttatggaaagctcgacacaatgcatactacgctgctcttgcaatgaga
acaggagcaagaggattcacgacagacgtatgtgtaccaatatcaaaactagcagatgtg
atatcagaaacaagatctgatctagatgaacatgaaattcttggaactgttgttggtcat
gttggtgatggaaatttccatgttatcctcccaacaattgaagaagataaaactgagcat
agaaagatccaatcgttttctgatcgtcttgtaagacgtgctctggctgcagatggaaca
tgcactggagaacatggaattggacttggaaaaagaaaatacttgagagaagaattagga
gagaatactgtacggttgatgcacacgatcaaacacgcgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACAGTTCAGATTCAAGCTTGCA 664 423 2122 Antisense
AGCTTGCAACTCTTACAGTAGCCTG 456 75 2139 Antisense
GTAGCCTGAACCTTCGAGAATCACC 392 457 2156 Antisense
AGAATCACCAACTCTTTTCATTGAA 709 73 2172 Antisense
TTGATTCTGGAGCCTCACCTGATAA 693 703 2279 Antisense
AAGCTCGACACAATGCATACTACGC 511 147 2321 Antisense
CATACTACGCTGCTCTTGCAATGAG 261 213 2336 Antisense
GGAAATTTCCATGTTATCCTCCCAA 475 527 2491 Antisense
ATCCAATCGTTTTCTGATCGTCTTG 317 41 2548 Antisense
TGTAAGACGTGCTCTGGCTGCAGAT 499 557 2571 Antisense
GATGCACACGATCAAACACGCGTTG 398 413 2679 Antisense
Sequence Source Affymetrix Proprietary Database